PMID- 33082827
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201022
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2020
DP  - 2020
TI  - BuShen HuoXue Decoction Promotes Decidual Stromal Cell Proliferation via the 
      PI3K/AKT Pathway in Unexplained Recurrent Spontaneous Abortion.
PG  - 6868470
LID - 10.1155/2020/6868470 [doi]
LID - 6868470
AB  - BuShen HuoXue decoction (BSHXD) has been used to treat patients with unexplained 
      recurrent spontaneous abortion (URSA). However, the chemical compounds and 
      mechanism by which BSHXD exerts its therapeutic and systemic effects to promote 
      the proliferation of decidual stromal cells (DSCs) has not been elucidated. This 
      work sought to elucidate the cellular and molecular mechanism of BSHXD in terms 
      of inflammatory factors IL-17A in DSCs in vitro because of the critical roles of 
      inflammation, apoptosis, and immunity in the development and progression of 
      pregnancy loss. Twelve migratory chemical compounds from BSHXD extract were 
      qualitatively analyzed by high-performance liquid chromatography (HPLC). DSCs 
      were collected from normal early pregnancy (NEP) and URSA to determine whether 
      BSHXD affects IL-17A/IL17RA via the PI3K/AKT pathway. Abnormal apoptosis and 
      activated p-AKT were observed in URSA DSCs. RhIL-17 A, LY294002 (a PI3K pathway 
      inhibitor), and BSHXD were individually or simultaneously administered in NEP 
      DSCs, suggesting that BSHXD restored cell proliferation without excessive 
      stimulation and IL-17A promotes proliferation via the PI3K/AKT pathway. Using the 
      same intervention in URSA DSCs, qRT-PCR measured the upregulated mRNA levels of 
      IL-17 A/IL-17RA, PI3K, AKT, p-AKT, PTEN, Bcl-2, and Bcl-xL and downregulated mRNA 
      levels of BAD and ACT1 after treatment with BSHXD. We demonstrated that BSHXD 
      affected IL-17A/IL-17R via PI3K/AKT pathway to promote the proliferative activity 
      of DSCs in URSA. These results provide a new insight to further clarify the 
      relationship between inflammation and apoptosis and the mechanism of imbalance in 
      the dynamic equilibrium between Th17/Treg immune cells at the maternal-fetal 
      interface.
CI  - Copyright (c) 2020 Xiaoling Feng et al.
FAU - Feng, Xiaoling
AU  - Feng X
AUID- ORCID: 0000-0002-8724-8855
AD  - The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 
      Harbin, China.
AD  - The Heilongjiang University of Chinese Medicine, Harbin, China.
FAU - Jiang, Sha
AU  - Jiang S
AUID- ORCID: 0000-0001-8440-7104
AD  - The Heilongjiang University of Chinese Medicine, Harbin, China.
FAU - Leung, WingTing
AU  - Leung W
AD  - Hospital and Institute of Obstetrics and Gynecology, Fudan University, Shanghai, 
      China.
AD  - The Academy of Integrative Medicine, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, 
      Shanghai, China.
FAU - Wang, Ling
AU  - Wang L
AD  - The Eighth Clinical Medical College of Beijing University of Chinese Medicine, 
      Xiamen, China.
FAU - Gober, Hans Jurgen
AU  - Gober HJ
AD  - Department of Pharmacy, Neuromed Campus, Kepler University Hospital, Linz 4020, 
      Austria.
FAU - Chen, Lu
AU  - Chen L
AD  - The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 
      Harbin, China.
FAU - Zhang, Yang
AU  - Zhang Y
AUID- ORCID: 0000-0003-1133-1510
AD  - The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 
      Harbin, China.
FAU - Wang, Ling
AU  - Wang L
AUID- ORCID: 0000-0003-4905-9903
AD  - Hospital and Institute of Obstetrics and Gynecology, Fudan University, Shanghai, 
      China.
AD  - The Academy of Integrative Medicine, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine-related Diseases, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20201005
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC7556073
COIS- There are no conflicts of interest regarding the submission of this manuscript.
EDAT- 2020/10/22 06:00
MHDA- 2020/10/22 06:01
PMCR- 2020/10/05
CRDT- 2020/10/21 06:01
PHST- 2020/04/28 00:00 [received]
PHST- 2020/07/08 00:00 [accepted]
PHST- 2020/10/21 06:01 [entrez]
PHST- 2020/10/22 06:00 [pubmed]
PHST- 2020/10/22 06:01 [medline]
PHST- 2020/10/05 00:00 [pmc-release]
AID - 10.1155/2020/6868470 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2020 Oct 5;2020:6868470. doi: 
      10.1155/2020/6868470. eCollection 2020.