PMID- 33085211
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20210609
IS  - 1099-0496 (Electronic)
IS  - 1099-0496 (Linking)
VI  - 56
IP  - 1
DP  - 2021 Jan
TI  - Effect of human umbilical cord-derived mesenchymal stem cells on murine model of 
      bronchiolitis obliterans like injury.
PG  - 129-137
LID - 10.1002/ppul.25128 [doi]
AB  - BACKGROUND: Bronchiolitis obliterans is a fatal respiratory disease characterized 
      by the obliteration of small airways. Mesenchymal stem cells (MSCs) is a 
      promising candidate for cell-based therapy. OBJECTIVE: To evaluate the 
      therapeutic effect of human umbilical cord-derived mesenchymal stem cells 
      (HUC-MSCs) on a murine model of bronchiolitis obliterans like injury (BOLI). 
      METHOD: The murine model of BOLI was established by administrating of diacetyl 
      (DA) via intratracheal instillation. Treatment of HUC-MSCs or HUC-MSCs culture 
      medium (HUC-MSCs-CM) was conducted in the BOLI model. RESULTS: The pathogenic 
      manifestations, lung function, and the number of neutrophils were similar between 
      the oropharyngeal inhalation DA group (OPI-DA), intratracheal instillation group 
      (ITI-DA); however, less reduction of weight and higher survival rate were 
      observed in ITI-DA groups. Compared with the control groups, the trend of weight 
      loss was significantly reduced (p < .05), and the pulmonary function was 
      significantly improved (p < .05) in HUC-MSCs and HUC-MSCs-CM groups. Masson 
      staining and hematoxylin and eosin staining showed that the deposition of 
      collagen around bronchioles and blood vessels is less and airway epithelial cells 
      and basal cells in lung tissue repaired better in HUC-MSCs and HUC-MSCs-CM groups 
      compared with the control groups. Immunofluorescence shows the expression of 
      E-cadherin and cytokeratin 5 (CK-5) were significantly higher in HUC-MSCs and 
      HUC-MSCs-CM groups compared with control groups, while HUC-MSCs themselves did 
      not express E-cadherin or CK-5. The DiI label showed HUC-MSCs gradually reduced 
      after 2 days in the bronchus and 4 days in bronchiole. CONCLUSION: HUC-MSCs could 
      help to repair airway epithelial cells in a murine model of BOLI. It might be 
      related to paracrine factors of HUC-MSCs.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Lin, Jilei
AU  - Lin J
AUID- ORCID: 0000-0003-1920-9317
AD  - Department of Respiratory Disease, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Deng, Huarong
AU  - Deng H
AD  - Guangzhou Women and Children's Medical Center, Guangdong, China.
FAU - Zhang, Yin
AU  - Zhang Y
AUID- ORCID: 0000-0002-9229-9542
AD  - Department of Respiratory Disease, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Zou, Lin
AU  - Zou L
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
AD  - Center for Clinical Molecular Medicine, Children's Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Fu, Zhou
AU  - Fu Z
AD  - Department of Respiratory Disease, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Dai, Jihong
AU  - Dai J
AUID- ORCID: 0000-0002-1520-6752
AD  - Department of Respiratory Disease, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201029
PL  - United States
TA  - Pediatr Pulmonol
JT  - Pediatric pulmonology
JID - 8510590
SB  - IM
MH  - Animals
MH  - *Bronchiolitis Obliterans
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - *Disease Models, Animal
MH  - Epithelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - HUC-MSCs
OT  - HUC-MSCs-CM
OT  - airway epithelial cells
OT  - bronchiolitis obliterans
EDAT- 2020/10/22 06:00
MHDA- 2021/06/10 06:00
CRDT- 2020/10/21 12:29
PHST- 2020/06/19 00:00 [received]
PHST- 2020/10/15 00:00 [revised]
PHST- 2020/10/16 00:00 [accepted]
PHST- 2020/10/22 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2020/10/21 12:29 [entrez]
AID - 10.1002/ppul.25128 [doi]
PST - ppublish
SO  - Pediatr Pulmonol. 2021 Jan;56(1):129-137. doi: 10.1002/ppul.25128. Epub 2020 Oct 
      29.