PMID- 33085231
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20220531
IS  - 2618-0456 (Electronic)
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 38
IP  - 5
DP  - 2020 Oct 1
TI  - [Effect of enhancer of zeste homolog 2 inhibitor GSK126 on the proliferation and 
      apoptosis of tongue squamous cell carcinoma].
PG  - 495-501
LID - 10.7518/hxkq.2020.05.004 [doi]
AB  - OBJECTIVE: This study aims to study the effect of the enhancer of zeste homolog 2 
      (EZH2) inhibitor GSK126 on the proliferation and apoptosis of human tongue 
      squamous cell carcinoma cells in vitro and explore its related mechanisms in 
      order to obtain insights into the clinical treatment of tongue squamous cell 
      carcinoma. METHODS: Different concentrations of GSK126 were applied to CAL-27 
      cells of tongue squamous cell carcinoma, and the effects of drugs on cell 
      proliferation were detected through methyl thiazolyl tetrazolium (MTT) assay, 
      colony formation assay, and 5-ethynyl-2'-deoxyuridine (EdU) fluorescence 
      staining. Hoechst33342 fluorescence staining and the JC-1 method were used in 
      observing apoptosis. The expression levels of extracellular regulated protein 
      kinases (ERK), phospho-extracellular regulated protein kinases (p-ERK), Bax, 
      Bcl-2, and Cleaved caspase-9 in Cal-27 cells were detected through Western blot. 
      RESULTS: GSK126 inhibited CAL-27 cell proliferation and promoted apoptosis. 
      GSK126 down-regulated the expression of p-ERK and Bcl-2 and increased the 
      expression of Bax and Cleaved caspase-9 (P<0.05). CONCLUSIONS: GSK126 can inhibit 
      the proliferation of CAL-27 cells in tongue squamous cell carcinoma and promote 
      its apoptosis, and the related mechanism may be associated with the inhibition of 
      the MEK/ERK signaling pathway and activation of the Bax/Bcl-2 pathway.
FAU - Liu, Jia-Nan
AU  - Liu JN
AD  - Second Affiliated Hospital of Jinzhou Medical University and School of 
      Stomatology, Jinzhou 121004, China.
FAU - Ma, Zhao-Lei
AU  - Ma ZL
AD  - Second Affiliated Hospital of Jinzhou Medical University and School of 
      Stomatology, Jinzhou 121004, China.
FAU - Su, Rong-Jian
AU  - Su RJ
AD  - Life Science Institute of Jinzhou Medical University, Jinzhou 121000, China.
FAU - Huang, Ke-Qiang
AU  - Huang KQ
AD  - Second Affiliated Hospital of Jinzhou Medical University and School of 
      Stomatology, Jinzhou 121004, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (GSK-2816126)
RN  - 0 (Indoles)
RN  - 0 (Pyridones)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - *Apoptosis
MH  - *Carcinoma, Squamous Cell/drug therapy
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Enhancer of Zeste Homolog 2 Protein
MH  - Humans
MH  - Indoles
MH  - Pyridones
MH  - *Tongue Neoplasms/drug therapy
PMC - PMC7573763
OTO - NOTNLM
OT  - GSK126
OT  - apoptosis
OT  - proliferation
OT  - tongue squamous cell carcinoma
COIS- 利益冲突声明：作者声明本文无利益冲突。
EDAT- 2020/10/22 06:00
MHDA- 2020/10/24 06:00
PMCR- 2020/10/01
CRDT- 2020/10/21 12:29
PHST- 2020/10/21 12:29 [entrez]
PHST- 2020/10/22 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - wcjs-38-05-495 [pii]
AID - 10.7518/hxkq.2020.05.004 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Oct 1;38(5):495-501. doi: 
      10.7518/hxkq.2020.05.004.