PMID- 33086260
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1536-481X (Electronic)
IS  - 1057-0829 (Linking)
VI  - 30
IP  - 2
DP  - 2021 Feb 1
TI  - Effects of Nonporous Silica Nanoparticles on Human Trabecular Meshwork Cells.
PG  - 195-202
LID - 10.1097/IJG.0000000000001709 [doi]
AB  - PRECIS: Silica nanoparticles (SiNPs), which are potential drug carriers for 
      glaucoma treatment, may induce mild dose-dependent cytotoxicity but not so severe 
      as to compromise a mammalian target of rapamycin (mTOR) pathway in immortalized 
      trabecular meshwork (TM) cells. PURPOSE: Nanoparticle-based ophthalmic drug 
      delivery is a promising field of drug development. In this study, we evaluated 
      the effect of nonporous SiNPs on human TM cells. METHODS: TM cells were exposed 
      to different concentrations (0 to 100 microg/mL) of SiNPs (50, 100, and 150 nm) for 
      up to 48 hours. Transmission electron microscopy confirmed the intracellular 
      distribution of SiNPs. Cellular viability assay, reactive oxygen species 
      generation, autophagy, and activation of the mTOR pathway were evaluated. 
      Histologic analysis of the TM structure was performed after intracameral 
      injection of SiNPs (0.05 mL of 200 microg/mL concentration) in rabbits. RESULTS: 
      SiNPs were taken up by TM cells and localized in the cytoplasm. Neither nuclear 
      entry nor mitochondrial damage was observed. SiNPs induced a mild but 
      dose-dependent increase of lactate dehydrogenase. However, neither increase of 
      intracellular reactive oxygen species levels nor apoptosis was observed after 
      SiNPs exposure. Significant coactivation of autophagy and the mTOR pathway were 
      observed with exposure to SiNPs. Aqueous plexus structure was well maintained 
      without inflammation in rabbits after SiNPs exposure. CONCLUSIONS: SiNPs induce 
      mild and dose-dependent cytotoxicity in TM cells. However, the toxicity level is 
      not enough to compromise the mTOR pathway of TM cells and histologic structure of 
      the aqueous plexus tissue.
CI  - Copyright (c) 2020 Wolters Kluwer Health, Inc. All rights reserved.
FAU - Kim, Martha
AU  - Kim M
AD  - Department of Ophthalmology, Dongguk University Ilsan Hospital, Goyang.
FAU - Park, Joo-Hee
AU  - Park JH
AD  - Department of Ophthalmology, Dongguk University Ilsan Hospital, Goyang.
AD  - Department of Biochemistry, College of Medicine, Dongguk University, Gyeongju.
FAU - Jeong, Hyejoong
AU  - Jeong H
AD  - Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 
      South Korea.
FAU - Hong, Jinkee
AU  - Hong J
AD  - Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 
      South Korea.
FAU - Park, Choul Yong
AU  - Park CY
AD  - Department of Ophthalmology, Dongguk University Ilsan Hospital, Goyang.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Glaucoma
JT  - Journal of glaucoma
JID - 9300903
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Humans
MH  - Intraocular Pressure
MH  - *Nanoparticles/toxicity
MH  - Rabbits
MH  - *Silicon Dioxide/toxicity
MH  - Trabecular Meshwork
COIS- Disclosure: The authors declare no conflict of interest.
EDAT- 2020/10/22 06:00
MHDA- 2021/09/04 06:00
CRDT- 2020/10/21 20:08
PHST- 2020/03/30 00:00 [received]
PHST- 2020/09/29 00:00 [accepted]
PHST- 2020/10/22 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2020/10/21 20:08 [entrez]
AID - 00061198-202102000-00014 [pii]
AID - 10.1097/IJG.0000000000001709 [doi]
PST - ppublish
SO  - J Glaucoma. 2021 Feb 1;30(2):195-202. doi: 10.1097/IJG.0000000000001709.