PMID- 33087851
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 2399-3642 (Electronic)
IS  - 2399-3642 (Linking)
VI  - 3
IP  - 1
DP  - 2020 Oct 21
TI  - teiresias, a Fruitless target gene encoding an immunoglobulin-superfamily 
      transmembrane protein, is required for neuronal feminization in Drosophila.
PG  - 598
LID - 10.1038/s42003-020-01327-z [doi]
LID - 598
AB  - This study aims at identifying transcriptional targets of FruitlessBM (FruBM), 
      which represents the major isoform of male-specific FruM transcription factors 
      that induce neural sexual dimorphisms. A promoter of the axon-guidance factor 
      gene robo1 carries the 16-bp palindrome motif Pal1, to which FruM binds. Our 
      genome-wide search for Pal1-homologous sequences yielded ~200 candidate genes. 
      Among these, CG17716 potentially encodes a transmembrane protein with 
      extracellular immunoglobulin (Ig)-like domains similar to Robo1. Indeed, FruBM 
      overexpression reduced CG17716 mRNA and protein expression. In the fru-expressing 
      mAL neuron cluster exhibiting sexual dimorphism, we found that CG17716 knockdown 
      in female neurons completely transformed all neurites to the male-type. 
      Conversely, CG17716 overexpression suppressed male-specific midline crossing of 
      fru-expressing sensory axons. We renamed CG17716 teiresias (tei) based on this 
      feminizing function. We hypothesize that Tei interacts with other Ig superfamily 
      transmembrane proteins, including Robo1, to feminize the neurite patterns in 
      females, whereas FruBM represses tei transcription in males.
FAU - Sato, Kosei
AU  - Sato K
AUID- ORCID: 0000-0002-4463-1440
AD  - Neuro-Network Evolution Project, Advanced ICT Research Institute, National 
      Institute of Information and Communications Technology, Kobe, Japan.
FAU - Ito, Hiroki
AU  - Ito H
AD  - Division of Neurogenetics, Tohoku University Graduate School of Life Sciences, 
      Sendai, Japan.
FAU - Yamamoto, Daisuke
AU  - Yamamoto D
AUID- ORCID: 0000-0002-4623-0106
AD  - Neuro-Network Evolution Project, Advanced ICT Research Institute, National 
      Institute of Information and Communications Technology, Kobe, Japan. 
      daichan@nict.go.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201021
PL  - England
TA  - Commun Biol
JT  - Communications biology
JID - 101719179
RN  - 0 (Drosophila Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Transcription Factors)
RN  - 0 (fru protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - Brain/metabolism
MH  - Drosophila/*genetics/metabolism
MH  - Drosophila Proteins/genetics/metabolism
MH  - Female
MH  - Feminization/*genetics
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation
MH  - Genetic Loci
MH  - Interneurons/metabolism
MH  - Male
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*metabolism
MH  - Protein Binding
MH  - Receptors, Immunologic/*genetics
MH  - Sex Characteristics
MH  - Transcription Factors/metabolism
PMC - PMC7578032
COIS- The authors declare no competing interests.
EDAT- 2020/10/23 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/10/21
CRDT- 2020/10/22 05:33
PHST- 2020/03/08 00:00 [received]
PHST- 2020/09/25 00:00 [accepted]
PHST- 2020/10/22 05:33 [entrez]
PHST- 2020/10/23 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/10/21 00:00 [pmc-release]
AID - 10.1038/s42003-020-01327-z [pii]
AID - 1327 [pii]
AID - 10.1038/s42003-020-01327-z [doi]
PST - epublish
SO  - Commun Biol. 2020 Oct 21;3(1):598. doi: 10.1038/s42003-020-01327-z.