PMID- 33088003
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211203
IS  - 0046-8991 (Print)
IS  - 0973-7715 (Electronic)
IS  - 0046-8991 (Linking)
VI  - 60
IP  - 4
DP  - 2020 Dec
TI  - Protective Functions of Group 3 Late Embryogenesis Abundant (G3LEA) Proteins in 
      Enterococcus faecium During Vancomycin Treatment.
PG  - 526-534
LID - 10.1007/s12088-020-00899-y [doi]
AB  - Late embryogenesis abundant (LEA) proteins protect organisms from various 
      environmental stresses; however, the underlying mechanism of LEA mediated 
      therapeutic evasion is still unclear in both eukaryotes and prokaryotes. In this 
      study, group 3 LEA protein (G3LEA) of vancomycin-resistant Enterococcus faecium 
      under sublethal concentration of vancomycin stress was evaluated and shown to 
      have two functions: the first is the reduction of reactive oxygen species (ROS) 
      content, preventing apoptosis by suppressing apoptotic proteins Cas3 and MAOB, 
      and the second is activating specific drug efflux pumps. Sublethal vancomycin 
      model was established with using Propidium Iodide (PI) stain. Real-time PCR was 
      conducted to evaluate the expression of G3lea. Flow cytometry and confocal 
      microscope using Anti- G3LEA, anti- MAOB, and anti- Cas3 were performed to assess 
      the expression of G3LEA. Under sublethal vancomycin stress, G3LEA is upregulated, 
      suppressing the expression of apoptotic markers and increasing specific efflux 
      markers. These results suggest that G3LEA protein suppresses antibiotic mediated 
      apoptosis in prokaryotic cells and plays a key role in understanding and 
      preventing antibiotic resistance.
CI  - (c) Association of Microbiologists of India 2020.
FAU - Song, Ahran
AU  - Song A
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Kim, Boyong
AU  - Kim B
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
AD  - Life Together, 13 Gongdan-ro, Chuncheon-siGangwon, South Korea.
AD  - Mitosbio, 13, Gongdan-ro, Chuncheon-si, Gangwon, South Korea.
FAU - Kim, Eunyoung
AU  - Kim E
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Sung, Jae Hwi
AU  - Sung JH
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Park, Yoonjin
AU  - Park Y
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Park, Sohyeon
AU  - Park S
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Park, Taegun
AU  - Park T
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
FAU - Kim, Jin Kwan
AU  - Kim JK
AD  - Department of Biomedical Laboratory Science, College of Health Science, Jungwon 
      University, 85 Munmu-ro, Goesan-eup, Goesan-gun, Chungbuk, South Korea. GRID: 
      grid.440940.d. ISNI: 0000 0004 0446 3336
FAU - Jeong, Yoonhwa
AU  - Jeong Y
AD  - Research Center for Industrialization of Natural Nutraceuticals, Dankook 
      University, Cheonan, Chungnam, Korea. GRID: grid.411982.7. ISNI: 0000 0001 0705 
      4288
AD  - Department of Food Science and Nutrition, College of Natural Science, Dankook 
      University, Cheonan, Chungnam, Korea. GRID: grid.411982.7. ISNI: 0000 0001 0705 
      4288
FAU - Lee, Seung Gwan
AU  - Lee SG
AD  - Department of Integrated Biomedical and Life Science, College of Health Science, 
      Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, South Korea. GRID: 
      grid.222754.4. ISNI: 0000 0001 0840 2678
LA  - eng
PT  - Journal Article
DEP - 20200716
PL  - India
TA  - Indian J Microbiol
JT  - Indian journal of microbiology
JID - 0374703
PMC - PMC7539248
OTO - NOTNLM
OT  - Apoptosis
OT  - Efflux pump
OT  - Enterococcus faecium
OT  - LEA proteins
OT  - ROS
OT  - Vancomycin
COIS- Conflicts of interestThe authors declare that they have no conflict of interest.
EDAT- 2020/10/23 06:00
MHDA- 2020/10/23 06:01
PMCR- 2021/12/01
CRDT- 2020/10/22 05:35
PHST- 2020/03/11 00:00 [received]
PHST- 2020/07/10 00:00 [accepted]
PHST- 2020/10/22 05:35 [entrez]
PHST- 2020/10/23 06:00 [pubmed]
PHST- 2020/10/23 06:01 [medline]
PHST- 2021/12/01 00:00 [pmc-release]
AID - 899 [pii]
AID - 10.1007/s12088-020-00899-y [doi]
PST - ppublish
SO  - Indian J Microbiol. 2020 Dec;60(4):526-534. doi: 10.1007/s12088-020-00899-y. Epub 
      2020 Jul 16.