PMID- 33089914
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20221005
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 24
IP  - 23
DP  - 2020 Dec
TI  - Phosphorylated STAT3 suppresses microRNA-19b/1281 to aggravate lung injury in 
      mice with type 2 diabetes mellitus-associated pulmonary tuberculosis.
PG  - 13763-13774
LID - 10.1111/jcmm.15954 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is a risk factor for pulmonary tuberculosis (PTB) 
      and increased mortality. This work focused on the functions of phosphorylated 
      STAT3 in lung injury in mouse with T2DM-associated PTB and the molecules 
      involved. A mouse model with T2DM-PTB was induced by administrations of 
      streptozotocin, nicotinamide and mycobacterium tuberculosis (Mtb). A 
      pSTAT3-specific inhibitor AG-490 was given into mice and then the lung injury in 
      mice was observed. The molecules involved in AG-490-mediated events were screened 
      out. Altered expression of miR-19b, miR-1281 and NFAT5 was introduced to identify 
      their involvements and roles in lung injury and PTB severity in the mouse model. 
      Consequently, pSTAT3 expression in mice with T2DM-associated PTB was increased. 
      Down-regulation of pSTAT3 by AG-490 prolonged the lifetime of mice and improved 
      the histopathologic conditions, and inhibited the fibrosis, inflammation, Mtb 
      content and number of apoptotic epithelial cells in mouse lung tissues. pSTAT3 
      transcriptionally suppressed miR-19b/1281 expression to up-regulate NFAT5. 
      Inhibition of miR-19b/1281 or up-regulation of NFAT5 blocked the protective roles 
      of AG-490 in mouse lung tissues. To conclude, this study evidenced that pSTAT3 
      promotes NFAT5 expression by suppressing miR-19b/1281 transcription, leading to 
      lung injury aggravation and severity in mice with T2DM-associated PTB.
CI  - (c) 2020 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Wang, Xianhua
AU  - Wang X
AUID- ORCID: 0000-0003-4155-7924
AD  - Department of Quality Control, Center for Disease Control and Prevention of 
      Changji Hui Autonomous Prefecture, Changji, China.
FAU - Lin, Yuefu
AU  - Lin Y
AD  - Department of Prevention, Linyi People's Hospital, Linyi, China.
FAU - Liang, Ying
AU  - Liang Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Xinjiang 
      Medical University, Urumqi, China.
FAU - Ye, Yang
AU  - Ye Y
AD  - Department of Laboratory, People's Hospital of Changji Hui Autonomous Prefecture, 
      Changji, China.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Tuberculosis, Center for Disease Control and Prevention of Changji 
      Hui Autonomous Prefecture, Changji, China.
FAU - Tai, Aer
AU  - Tai A
AD  - Department of Respiratory Medicine, Xinjiang Uygur Autonomous Region Chest 
      Hospital, Urumqi, China.
FAU - Wu, Shuimiao
AU  - Wu S
AD  - Department of Respiratory and Critical Care Medicine, Weinan Central Hospital, 
      Weinan, China.
FAU - Pan, Jian
AU  - Pan J
AD  - Department of Respiratory and Critical Care Medicine, The Third People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201022
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Biomarkers)
RN  - 0 (MIRN19 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (STAT3 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Biomarkers
MH  - Biopsy
MH  - Diabetes Mellitus, Type 2/*complications
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Immunohistochemistry
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Phosphorylation
MH  - STAT3 Transcription Factor/*metabolism
MH  - Tuberculosis, Pulmonary/*etiology/*metabolism/pathology
PMC - PMC7754065
OTO - NOTNLM
OT  - NAFT5
OT  - Type 2 diabetes mellitus
OT  - inflammation
OT  - microRNA-1281
OT  - microRNA-19b
OT  - phosphorylated STAT3
OT  - pulmonary tuberculosis
COIS- The authors declare no potential conflicts of interest.
EDAT- 2020/10/23 06:00
MHDA- 2021/05/15 06:00
PMCR- 2020/12/01
CRDT- 2020/10/22 08:49
PHST- 2020/05/14 00:00 [received]
PHST- 2020/08/09 00:00 [revised]
PHST- 2020/08/19 00:00 [accepted]
PHST- 2020/10/23 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/10/22 08:49 [entrez]
PHST- 2020/12/01 00:00 [pmc-release]
AID - JCMM15954 [pii]
AID - 10.1111/jcmm.15954 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2020 Dec;24(23):13763-13774. doi: 10.1111/jcmm.15954. Epub 2020 
      Oct 22.