PMID- 33090483
OWN - NLM
STAT- MEDLINE
DCOM- 20210215
LR  - 20210215
IS  - 1365-2591 (Electronic)
IS  - 0143-2885 (Linking)
VI  - 54
IP  - 3
DP  - 2021 Mar
TI  - Vesicular glutamate transporter-immunopositive axons that coexpress neuropeptides 
      in the rat and human dental pulp.
PG  - 377-387
LID - 10.1111/iej.13427 [doi]
AB  - AIM: To examine the type of vesicular glutamate transporter 
      (VGLUT)-immunopositive (+) axons that coexpress neuropeptides in the rat and 
      human dental pulp, which may help understand peripheral mechanism of pulpal 
      inflammatory pain in rats and humans. METHODOLOGY: The trigeminal ganglia (TG) 
      and the dental pulp of the maxillary molar teeth from three male Sprague-Dawley 
      rats weighing 300-330 g and dental pulps of three healthy human (male) maxillary 
      premolar teeth from three 16 to 28-year-old patients extracted for orthodontic 
      treatment were used. The type of VGLUT + axons that coexpress substance P (SP)- 
      and/or calcitonin gene-related peptide (CGRP) and parvalbumin in the rat TG and 
      in the axons of the rat and the human dental pulp was examined by double 
      fluorescence immunohistochemistry and quantitative analysis. Results were 
      analyzed using one-way anova and the Kruskal-Wallis test. RESULTS: SP and CGRP 
      were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG 
      neurons and pulpal axons (P < 0.05). SP and CGRP were expressed in a considerable 
      number of human VGLUT2 + pulpal axons and also in many rat TG neurons and pulpal 
      axons. The fraction of VGLUT1 + axons expressing parvalbumin was about three 
      times higher in the rat than in the human dental pulp (P < 0.05). CONCLUSIONS: 
      These findings suggest that the types of VGLUT + axons, which release 
      neuropeptides, may be different between the rat and the human dental pulp, 
      raising a possibility that peripheral mechanism of pulpal inflammatory pain may 
      be different between rats and humans.
CI  - (c) 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.
FAU - Cho, Y S
AU  - Cho YS
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
FAU - Ko, H G
AU  - Ko HG
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
FAU - Han, H M
AU  - Han HM
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
FAU - Park, S K
AU  - Park SK
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
FAU - Moozhayil, S J
AU  - Moozhayil SJ
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
FAU - Choi, S Y
AU  - Choi SY
AD  - Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook 
      National University, Daegu, South Korea.
FAU - Bae, Y C
AU  - Bae YC
AUID- ORCID: 0000-0002-7618-418X
AD  - Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National 
      University, Daegu, South Korea.
LA  - eng
GR  - NRF-2017R1A2B2003561/National Research Foundation of Korea/
GR  - NRF-2017R1A5A2015391/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20201104
PL  - England
TA  - Int Endod J
JT  - International endodontic journal
JID - 8004996
RN  - 0 (Neuropeptides)
RN  - 0 (Vesicular Glutamate Transport Proteins)
MH  - Animals
MH  - Axons
MH  - Dental Pulp
MH  - Humans
MH  - *Neuropeptides
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Vesicular Glutamate Transport Proteins
OTO - NOTNLM
OT  - dental pulp
OT  - glutamate
OT  - inflammatory pain
OT  - neuropeptide
OT  - vesicular glutamate transporter
EDAT- 2020/10/23 06:00
MHDA- 2021/02/16 06:00
CRDT- 2020/10/22 12:19
PHST- 2020/05/25 00:00 [received]
PHST- 2020/10/05 00:00 [revised]
PHST- 2020/10/05 00:00 [accepted]
PHST- 2020/10/23 06:00 [pubmed]
PHST- 2021/02/16 06:00 [medline]
PHST- 2020/10/22 12:19 [entrez]
AID - 10.1111/iej.13427 [doi]
PST - ppublish
SO  - Int Endod J. 2021 Mar;54(3):377-387. doi: 10.1111/iej.13427. Epub 2020 Nov 4.