PMID- 33091677
OWN - NLM
STAT- MEDLINE
DCOM- 20210426
LR  - 20210426
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1158
DP  - 2020 Nov 20
TI  - Simple and reliable serotonin assay in human serum by LC-MS/MS method coupled 
      with one step protein precipitation for clinical testing in patients with 
      carcinoid tumors.
PG  - 122395
LID - S1570-0232(20)31271-X [pii]
LID - 10.1016/j.jchromb.2020.122395 [doi]
AB  - Serotonin (5-hydroxytryptamine, 5-HT) is readily secreted in patients with 
      carcinoid tumors, especially arising from the midgut. Although serotonin assay in 
      human plasma or whole blood has been extensively studied, serotonin assay in 
      human serum by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has 
      received much less attention. In this study, a simple and reliable LC-MS/MS 
      method based on one step protein precipitation for sample pretreatment was 
      developed for clinical assay of serum serotonin. Namely, 150 muL of serum was 
      mixed with 50 muL of internal standard (IS) and 200 muL of 4 wt.% 5-sulfosalicylic 
      acid (SSA) solution for protein precipitation. The supernatant after 
      centrifugation was analyzed directly without further treatment. This method was 
      validated for consistent linearity from 0.94 to 240 ng/mL with CVs </= 11.7%, good 
      recovery in the range of 87.5%-104%, excellent analyte stability and low 
      carryover. No obvious matrix effect was observed. Intra- and inter-day 
      imprecision were below 8.03% and 11.5% respectively. Dilution linearity was 
      verified with satisfying linearly dependent coefficients (r(2) = 0.9937). The 
      reference interval of serotonin was established from 126 results derived from 
      subjects without carcinoid tumors. Therefore, apart from development of a serum 
      serotonin assay by the LC-MS/MS method, the reference interval (RI) of 5-HT has 
      also been established for clinical testing in patients with carcinoid tumors. In 
      addition, this method has been successfully used in our laboratory, indicating 
      that this robust LC-MS/MS assay with simple sample preparation and short analysis 
      time could offer inspiring potential for clinical testing of 5-HT in routine 
      clinical laboratories.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Shen, Ying
AU  - Shen Y
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Luo, Xia
AU  - Luo X
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Li, Huijun
AU  - Li H
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Chen, Zhongju
AU  - Chen Z
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Guan, Qing
AU  - Guan Q
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Cheng, Liming
AU  - Cheng L
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic 
      address: chengliming2015@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201006
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Carcinoid Tumor
MH  - Chemical Precipitation
MH  - Child
MH  - Child, Preschool
MH  - Chromatography, Liquid/*methods
MH  - Female
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Limit of Detection
MH  - Linear Models
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Serotonin/*blood/chemistry/isolation & purification
MH  - Tandem Mass Spectrometry/*methods
MH  - Young Adult
OTO - NOTNLM
OT  - Carcinoid tumor
OT  - LC-MS/MS
OT  - Protein precipitation
OT  - Serotonin
EDAT- 2020/10/23 06:00
MHDA- 2021/04/27 06:00
CRDT- 2020/10/22 20:13
PHST- 2020/05/15 00:00 [received]
PHST- 2020/09/17 00:00 [revised]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/10/23 06:00 [pubmed]
PHST- 2021/04/27 06:00 [medline]
PHST- 2020/10/22 20:13 [entrez]
AID - S1570-0232(20)31271-X [pii]
AID - 10.1016/j.jchromb.2020.122395 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Nov 20;1158:122395. doi: 
      10.1016/j.jchromb.2020.122395. Epub 2020 Oct 6.