PMID- 33092416
OWN - NLM
STAT- MEDLINE
DCOM- 20220609
LR  - 20220609
IS  - 1525-6014 (Electronic)
IS  - 0148-0545 (Linking)
VI  - 45
IP  - 4
DP  - 2022 Jul
TI  - Phytochemical evaluation of Cucumis prophetarum: protective effects against 
      carrageenan-induced prostatitis in rats.
PG  - 1461-1469
LID - 10.1080/01480545.2020.1838538 [doi]
AB  - Phytochemical study of the MeOH extract of Cucumis prophetarum fruits (family 
      Cucurbitaceae) by using different chromatographic techniques led to the isolation 
      of three metabolites; spinasterol (1), cucurbitacin B (2), and 
      2-O-beta-D-glucopyranosylcucurbitacin E (3). Their chemical structures were created 
      on the basis of physical, chemical, spectroscopic data 1D ((1)H and (13)C NMR), 
      and 2D NMR (HSQC and HMBC), as well as similarity with literature data. 
      Cucurbitacin B (Cu-B) (2) was found to be the major constituent. Potential 
      protective activities of MeOH extract, CHCl(3), and EtOAc fractions and Cu-B were 
      evaluated against carrageenan-induced prostatic inflammation in rats. Acute 
      toxicity was assessed by evaluating LD(50). Pretreatment with CHCl(3) fraction 
      and Cu-B ameliorated the rise in the prostate index and obviously protected 
      against histopathological changes. Further, MeOH, extract, CHCl(3), and EtOAc 
      fractions as well as Cu-B significantly protected against oxidative stress in 
      prostatic tissues. The anti-inflammatory activities of the extract, fractions and 
      Cu-B were confirmed by ameliorating the rise in prostatic content of the 
      inflammatory mediators TNF-alpha, IL-1beta, COX-2, and iNOS induced by carrageenan. In 
      addition, the rise in the chemotactic factors were myeloperoxidase (MPO), F4-80, 
      and monocyte chemoattractant protein-1 (MCP-1) was significantly hampered. In 
      conclusion, three known compounds (1-3) were isolated from Cucumis prophetarum 
      fruits. Cu-B (2) was the major identified compound. Particularly, CHCl(3) 
      fraction and isolated Cu-B exhibited potent anti-inflammatory activity against 
      carrageenan-induced prostatitis. The anti-inflammatory activity can be 
      attributed, at least partly, to inhibition of neutrophil and macrophage 
      infiltration into prostatic tissues.
FAU - Aljohani, Omar Saad
AU  - Aljohani OS
AD  - Faculty of Pharmacy, Department of Natural Products and Alternative Medicine, 
      King Abdulaziz University, Jeddah, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20201022
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - 9000-07-1 (Carrageenan)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Carrageenan/toxicity
MH  - *Cucumis
MH  - Humans
MH  - Male
MH  - Phytochemicals
MH  - Plant Extracts/chemistry/pharmacology/therapeutic use
MH  - *Prostatitis/chemically induced/drug therapy/prevention & control
MH  - Rats
OTO - NOTNLM
OT  - Cucumis prophetarum
OT  - Cucurbitaceae
OT  - cucurbitacin B
OT  - prostatitis
EDAT- 2020/10/24 06:00
MHDA- 2022/06/10 06:00
CRDT- 2020/10/23 05:30
PHST- 2020/10/24 06:00 [pubmed]
PHST- 2022/06/10 06:00 [medline]
PHST- 2020/10/23 05:30 [entrez]
AID - 10.1080/01480545.2020.1838538 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 2022 Jul;45(4):1461-1469. doi: 10.1080/01480545.2020.1838538. 
      Epub 2020 Oct 22.