PMID- 33093883
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201024
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 20
IP  - 6
DP  - 2020 Dec
TI  - Human umbilical cord-derived mesenchymal stem cells and human cord blood 
      mononuclear cells protect against cisplatin-induced acute kidney injury in rat 
      models.
PG  - 145
LID - 10.3892/etm.2020.9274 [doi]
LID - 145
AB  - Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are a promising 
      tool to attenuate cisplatin (CP)-induced acute kidney injury (AKI). However, 
      whether the transplantation of human cord blood mononuclear cells (hCBMNCs) 
      exhibits similar protective effects and their potential underlying mechanisms of 
      action remain unclear. The present study aimed to determine the protective 
      effects of hUCMSCs and hCBMNCs transplantation therapies on an established 
      CP-induced rat model and explore their underlying mechanisms of action. A total 
      of 24 Sprague-Dawley rats, selected based on body weight, were randomly assigned 
      into 4 groups: i) normal control; ii) model (CP); iii) hCBMNCs (CP + hCBMNCs); 
      and iv) hUCMSCs (CP + hUCMSCs). hUCMSCs (2.0x10(6) cells) and hCBMNCs (2.0x10(6) 
      cells) were injected into the femoral vein of rats 24 h after CP (8 mg/kg) 
      treatment. To determine the effects of hCBMNCs and hUCMSCs on CP-induced rats, 
      renal function assessment and histological evaluations were performed. Expression 
      levels of high mobility group box 1 (HMGB1) and the ratio of Bax/Bcl2 in renal 
      tissues were detected to elucidate their underlying molecular mechanisms of 
      action. The results demonstrated that transplantation of hUCMSCs and hCBMNCs 
      significantly improved renal function in CP-induced AKI rats, as evidenced by the 
      enhancement of renal morphology; decreased concentrations of blood urea nitrogen 
      and serum creatinine; and a lower percentage of apoptotic renal tubular cells. 
      The expression of HMGB1 and the ratio of Bax/Bcl-2 were significantly reduced in 
      the hUCMSCs and hCBMNCs groups compared with CP group. In conclusion, the present 
      study indicated that hCBMNCs exert similar protective effects to hUCMSCs on 
      CP-induced AKI. hUCMSCs and hCBMNCs protect against CP-induced AKI by suppressing 
      HMGB1 expression and preventing cell apoptosis.
CI  - Copyright: (c) Xu et al.
FAU - Xu, Qian
AU  - Xu Q
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Yan, Ping
AU  - Yan P
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Duan, Xiang-Jie
AU  - Duan XJ
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Wu, Xi
AU  - Wu X
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Chen, Xiao-Jun
AU  - Chen XJ
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Luo, Min
AU  - Luo M
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Peng, Jing-Cheng
AU  - Peng JC
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Feng, Li-Xin
AU  - Feng LX
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Liu, Jie
AU  - Liu J
AD  - Translational Center for Stem Cell Research, Tongji Hospital, Tongji University 
      School of Medicine, Shanghai, 200065, P.R. China.
FAU - Zhong, Hui-Lin
AU  - Zhong HL
AD  - Neuromedical Research Center, Guangdong 999 Brain Hospital, Guangzhou, Guangdong 
      510510, P.R. China.
FAU - Cheng, Wei
AU  - Cheng W
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
FAU - Zou, Qing-Yan
AU  - Zou QY
AD  - Neuromedical Research Center, Guangdong 999 Brain Hospital, Guangzhou, Guangdong 
      510510, P.R. China.
FAU - Duan, Shao-Bin
AU  - Duan SB
AD  - Department of Nephrology, The Second Xiangya Hospital, Central South University, 
      Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan 
      410011, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20201005
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7571324
OTO - NOTNLM
OT  - acute kidney injury
OT  - apoptosis
OT  - cisplatin
OT  - high mobility group box 1
OT  - stem cells
EDAT- 2020/10/24 06:00
MHDA- 2020/10/24 06:01
PMCR- 2020/10/05
CRDT- 2020/10/23 05:57
PHST- 2019/05/20 00:00 [received]
PHST- 2020/07/23 00:00 [accepted]
PHST- 2020/10/23 05:57 [entrez]
PHST- 2020/10/24 06:00 [pubmed]
PHST- 2020/10/24 06:01 [medline]
PHST- 2020/10/05 00:00 [pmc-release]
AID - ETM-0-0-09274 [pii]
AID - 10.3892/etm.2020.9274 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Dec;20(6):145. doi: 10.3892/etm.2020.9274. Epub 2020 Oct 5.