PMID- 33096854
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201030
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 10
IP  - 10
DP  - 2020 Oct 21
TI  - Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) 
      Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile 
      Fabrics.
LID - 10.3390/nano10102082 [doi]
LID - 2082
AB  - An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy 
      medicinal plant leaves of Mentha longifolia L. and used for the green synthesis 
      of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and 
      proteins. UV-vis spectroscopy, Fourier-transform infrared (FT-IR), transmission 
      electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering 
      (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis 
      results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs 
      with a particle size of 13-40 nm. Further, the stability of the Ag-NPs was 
      assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month 
      or by measuring the NPs surface charge (-19.2 mV) by zeta potential analysis (zeta). 
      The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at 
      different concentrations (6.25-100 ppm) against the pathogens Staphylococcus 
      aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and 
      Salmonella typhimurium with a clear inhibition zone ranging from (9.5 +/- 0.4) nm 
      to (21.7 +/- 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high 
      efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory 
      concentration (IC(50)) = 5.7 +/- 0.2 ppm). With respect to antibacterial and 
      in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected 
      as a safe dose for loading onto cotton fabrics. The scanning electron microscopy 
      connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the 
      nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric 
      elements. Moreover, the nano-finished fabrics exhibited more activity against 
      pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing 
      cycles, indicating the stability of the treated fabrics.
FAU - Salem, Salem S
AU  - Salem SS
AUID- ORCID: 0000-0003-2898-6708
AD  - Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 
      Nasr City, Cairo 11884, Egypt.
FAU - El-Belely, Ehab F
AU  - El-Belely EF
AUID- ORCID: 0000-0002-5352-4241
AD  - Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 
      Nasr City, Cairo 11884, Egypt.
FAU - Niedbala, Gniewko
AU  - Niedbala G
AUID- ORCID: 0000-0003-3721-6473
AD  - Department of Biosystems Engineering, Faculty of Environmental Engineering and 
      Mechanical Engineering, Poznan University of Life Sciences, Wojska Polskiego 50, 
      60-627 Poznan, Poland.
FAU - Alnoman, Maryam M
AU  - Alnoman MM
AD  - Biology Department, Faculty of Science, Taibah University, Al-Sharm, Yanbu 
      El-Bahr 46429, Saudi Arabia.
FAU - Hassan, Saad El-Din
AU  - Hassan SE
AUID- ORCID: 0000-0002-8443-1275
AD  - Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 
      Nasr City, Cairo 11884, Egypt.
FAU - Eid, Ahmed Mohamed
AU  - Eid AM
AUID- ORCID: 0000-0001-6043-647X
AD  - Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 
      Nasr City, Cairo 11884, Egypt.
FAU - Shaheen, Tharwat I
AU  - Shaheen TI
AD  - National Research Centre, El-Behouth St., Dokki, Giza 12622, Egypt.
FAU - Elkelish, Amr
AU  - Elkelish A
AUID- ORCID: 0000-0002-1683-8436
AD  - Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, 
      Egypt.
FAU - Fouda, Amr
AU  - Fouda A
AUID- ORCID: 0000-0003-3840-7837
AD  - Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 
      Nasr City, Cairo 11884, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20201021
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7589671
OTO - NOTNLM
OT  - Ag-NPs
OT  - antibacterial and cytotoxicity activities
OT  - cotton fabrics
OT  - endophytic actinomycetes
OT  - green synthesis
OT  - streptomyces spp.
COIS- The authors declare no conflict of interest.
EDAT- 2020/10/25 06:00
MHDA- 2020/10/25 06:01
PMCR- 2020/10/21
CRDT- 2020/10/24 01:01
PHST- 2020/10/01 00:00 [received]
PHST- 2020/10/16 00:00 [revised]
PHST- 2020/10/17 00:00 [accepted]
PHST- 2020/10/24 01:01 [entrez]
PHST- 2020/10/25 06:00 [pubmed]
PHST- 2020/10/25 06:01 [medline]
PHST- 2020/10/21 00:00 [pmc-release]
AID - nano10102082 [pii]
AID - nanomaterials-10-02082 [pii]
AID - 10.3390/nano10102082 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2020 Oct 21;10(10):2082. doi: 10.3390/nano10102082.