PMID- 33097641
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20211204
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 40
IP  - 48
DP  - 2020 Nov 25
TI  - Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal 
      Development.
PG  - 9169-9185
LID - 10.1523/JNEUROSCI.0929-20.2020 [doi]
AB  - Myosin X (Myo X) transports cargos to the tips of filopodia for cell adhesion, 
      migration, and neuronal axon guidance. Deleted in Colorectal Cancer (DCC) is one 
      of the Myo X cargos that is essential for Netrin-1-regulated axon pathfinding. 
      The function of Myo X in axon development in vivo and the underlying mechanisms 
      remain elusive. Here, we provide evidence for the role of Myo X in 
      Netrin-1-DCC-regulated axon development in developing mouse neocortex. The 
      knockout (KO) or knockdown (KD) of Myo X in cortical neurons of embryonic mouse 
      brain impairs axon initiation and contralateral branching/targeting. Similar axon 
      deficits are detected in Netrin-1-KO or DCC-KD cortical neurons. Further 
      proteomic analysis of Myo X binding proteins identifies KIF13B (a kinesin family 
      motor protein). The Myo X interaction with KIF13B is induced by Netrin-1. 
      Netrin-1 promotes anterograde transportation of Myo X into axons in a 
      KIF13B-dependent manner. KIF13B-KD cortical neurons exhibit similar axon 
      deficits. Together, these results reveal Myo X-KIF13B as a critical pathway for 
      Netrin-1-promoted axon initiation and branching/targeting.SIGNIFICANCE STATEMENT 
      Netrin-1 increases Myosin X (Myo X) interaction with KIF13B, and thus promotes 
      axonal delivery of Myo X and axon initiation and contralateral branching in 
      developing cerebral neurons, revealing unrecognized functions and mechanisms 
      underlying Netrin-1 regulation of axon development.
CI  - Copyright (c) 2020 Yu et al.
FAU - Yu, Hua-Li
AU  - Yu HL
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
AD  - Institute of Cytology and Genetics, Northeast Normal University, Changchun 
      130024, People's Republic of China.
FAU - Peng, Yun
AU  - Peng Y
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
FAU - Zhao, Yang
AU  - Zhao Y
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Institute of Cytology and Genetics, Northeast Normal University, Changchun 
      130024, People's Republic of China.
FAU - Lan, Yong-Sheng
AU  - Lan YS
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
FAU - Wang, Bo
AU  - Wang B
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
FAU - Zhao, Lu
AU  - Zhao L
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
AD  - Institute of Cytology and Genetics, Northeast Normal University, Changchun 
      130024, People's Republic of China.
FAU - Sun, Dong
AU  - Sun D
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
FAU - Pan, Jin-Xiu
AU  - Pan JX
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
FAU - Dong, Zhao-Qi
AU  - Dong ZQ
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
FAU - Mei, Lin
AU  - Mei L
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
FAU - Ding, Yu-Qiang
AU  - Ding YQ
AD  - Institute of Brain Sciences, Fudan University, Shanghai 200031, People's Republic 
      of China.
FAU - Zhu, Xiao-Juan
AU  - Zhu XJ
AUID- ORCID: 0000-0001-8386-8064
AD  - Institute of Cytology and Genetics, Northeast Normal University, Changchun 
      130024, People's Republic of China Wen-Cheng.Xiong@case.edu zhuxj720@nenu.edu.cn.
FAU - Xiong, Wen-Cheng
AU  - Xiong WC
AUID- ORCID: 0000-0001-9071-7598
AD  - Department of Neurosciences, School of Medicine, Case Western Reserve University, 
      Cleveland, Ohio 44106 Wen-Cheng.Xiong@case.edu zhuxj720@nenu.edu.cn.
AD  - Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, 
      Augusta University, Augusta, Georgia 30912.
LA  - eng
GR  - R01 AG045781/AG/NIA NIH HHS/United States
GR  - RF1 AG045781/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20201023
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (DCC Receptor)
RN  - 0 (Dcc protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Myo10 protein, mouse)
RN  - 0 (Ntn1 protein, mouse)
RN  - 158651-98-0 (Netrin-1)
RN  - EC 3.6.1.- (KIF13b protein, mouse)
RN  - EC 3.6.4.1 (Myosins)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/growth & development
MH  - DCC Receptor/genetics/physiology
MH  - Female
MH  - Kinesins/genetics/*physiology
MH  - Male
MH  - Membrane Proteins/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myosins/genetics/*physiology
MH  - Neocortex/cytology/growth & development
MH  - Netrin-1/genetics/*physiology
MH  - Pregnancy
PMC - PMC7687062
OTO - NOTNLM
OT  - KIF13B
OT  - Myo X
OT  - Netrin-1
OT  - axon
OT  - axon branching
OT  - axon initiation
EDAT- 2020/10/25 06:00
MHDA- 2021/02/17 06:00
PMCR- 2020/11/25
CRDT- 2020/10/24 05:29
PHST- 2020/04/23 00:00 [received]
PHST- 2020/09/04 00:00 [revised]
PHST- 2020/10/17 00:00 [accepted]
PHST- 2020/10/25 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/10/24 05:29 [entrez]
PHST- 2020/11/25 00:00 [pmc-release]
AID - JNEUROSCI.0929-20.2020 [pii]
AID - JN-RM-0929-20 [pii]
AID - 10.1523/JNEUROSCI.0929-20.2020 [doi]
PST - ppublish
SO  - J Neurosci. 2020 Nov 25;40(48):9169-9185. doi: 10.1523/JNEUROSCI.0929-20.2020. 
      Epub 2020 Oct 23.