PMID- 33098390
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220417
IS  - 2008-076X (Print)
IS  - 2008-0778 (Electronic)
IS  - 2008-0778 (Linking)
VI  - 14
IP  - 3
DP  - 2020 Oct
TI  - Zinc Protects against MDMA-Induced Apoptosis of Sertoli Cells in Mouse via 
      Attenuation of Caspase-3.
PG  - 223-227
LID - 10.22074/ijfs.2020.44410 [doi]
AB  - BACKGROUND: 3,4-Methylenedioxymethamphetamine (MDMA) disrupts function of the 
      endocrine system and different organs such as heart, blood vessels, kidney, liver 
      and nervous systems. This revision was conducted to evaluate impact of MDMA on 
      apoptosis and Zinc in the MDMA-induced apoptosis of cultured Sertoli cells by 
      measuring Caspase-3 gene expression. MATERIALS AND METHODS: In this experimental 
      study, Sertoli cells were incubated with MDMA (0, 0.5, 1, 3 and 5 mM), Zinc (0, 
      8, 16, 32, 64 muM) and Zinc (8 muM) prior to adding MDMA (5 mM) for 24 and 48 
      hours. MTT assay was used for evaluating impacts of these conditions on the 
      viability of Sertoli cells. Caspase-3 gene expression level was detected using 
      quantitative reverse transcription PCR (qRT-PCR) in all of the tested groups. 
      RESULTS: Finding showed that cellular viability was decreased and level of 
      Caspase-3 mRNA was increased in MDMA treated cells. Additionally, pre-treatment 
      with Zinc (8 muM) attenuated MDMA-induced apoptosis and down-regulated caspase-3. 
      The mean of caspase-3 mRNA level (fold change +/- SE) was 3.98 +/- 1.18, 0.31 +/- 0.28, 
      and 1.72 +/- 0.28 in respectively MDMA (5 mM), Zinc (8 muM), and Zinc+MDMA groups 
      vs. control group. The mean of Caspase-3 mRNA (fold change) was not statistically 
      different in the tested groups (P>0.05), unless MDMA (5 mM) group (P=0.008). 
      CONCLUSION: We suggest that MDMA toxicity could be involved in apoptosis of 
      Sertoli cells. In addition, Zinc could reduce MDMA-induced apoptosis by 
      down-regulation of Caspase-3 mRNA levels.
CI  - Copyright(c) by Royan Institute. All rights reserved.
FAU - Hossein-Zadeh, Nadia
AU  - Hossein-Zadeh N
AD  - Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
FAU - Bagheri, Morteza
AU  - Bagheri M
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran. Electronic Address: 
      mortezabagheri@umsu.ac.ir.
FAU - Abdi Rad, Isa
AU  - Abdi Rad I
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran.
FAU - Lozeie, Marziyeh
AU  - Lozeie M
AD  - Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
FAU - Nasir-Zadeh, Mahdieh
AU  - Nasir-Zadeh M
AD  - Cellular and Molecular Research Center, Cellular and Molecular Medicine 
      Institute, Urmia University of Medical Sciences, Urmia, Iran.
LA  - eng
PT  - Journal Article
DEP - 20201012
PL  - Iran
TA  - Int J Fertil Steril
JT  - International journal of fertility & sterility
JID - 101487941
PMC - PMC7604711
OTO - NOTNLM
OT  - Apoptosis
OT  - Caspase-3
OT  - MDMA
OT  - Sertoli Cells
OT  - Zinc
COIS- The authors declare no conflicts of interest.
EDAT- 2020/10/25 06:00
MHDA- 2020/10/25 06:01
PMCR- 2020/10/01
CRDT- 2020/10/24 08:35
PHST- 2020/01/01 00:00 [received]
PHST- 2020/05/26 00:00 [accepted]
PHST- 2020/10/24 08:35 [entrez]
PHST- 2020/10/25 06:00 [pubmed]
PHST- 2020/10/25 06:01 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 10.22074/ijfs.2020.44410 [doi]
PST - ppublish
SO  - Int J Fertil Steril. 2020 Oct;14(3):223-227. doi: 10.22074/ijfs.2020.44410. Epub 
      2020 Oct 12.