PMID- 33103411
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20210114
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 34
IP  - 5
DP  - 2020 Sep-Oct,
TI  - Remifentanil protects neurological function of rats with cerebral 
      ischemia-reperfusion injury via NR2B/CaMKIIalpha signaling pathway.
PG  - 1647-1656
LID - 10.23812/20-169-A [doi]
AB  - The effects of remifentanil were studied on cerebral ischemia-reperfusion injury 
      (IRI) in rats, and its regulatory effect was explored on the N-methyl D-aspartate 
      receptor subtype 2B (NR2B)/calcium/calmodulin-dependent protein kinase type II 
      subunit alpha (CaMKII) signaling pathway in cerebral tissues. A total of 40 
      Sprague-Dawley rats were randomly assigned into sham group, model group, low-dose 
      group (remifentanil injected into the caudal veins at 2 mug/kg) and high-dose 
      group (remifentanil injected into the caudal veins at 10 mug/kg). Then, in the 
      model, low-dose and high-dose groups, the rat model of cerebral IRI was 
      established through middle cerebral artery occlusion (namely, ischemia for 1 h 
      and reperfusion for 2 h), while no thread was inserted into the rats in the sham 
      group. Neurological function of rats in each group was evaluated, and the 
      cerebral infarct size was measured using 2,3,5-triphenyltetrazolium chloride 
      (TTC) staining. Moreover, terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) staining was performed to determine the neuronal 
      apoptosis level, and mitochondrial membrane potential was measured via JC-1 
      assay. Moreover, the reactive oxygen species (ROS) level in neurons and the 
      malondialdehyde (MDA) content in cerebral tissues were determined using 
      2',7'-dichlorofluorescin diacetate (DCFH-DA) assay and enzyme-linked 
      immunosorbent assay (ELISA), respectively. Finally, the expression levels of 
      apoptosis-associated proteins and the NR2B/CaMKIIalpha signaling pathway-associated 
      proteins in cerebral tissues were measured by Western blotting. Remifentanil 
      preconditioning substantially decreased the neurological score of rats (p<0.01), 
      cerebral infarct size (p<0.01), neuronal apoptosis level (p<0.01), ROS level in 
      neurons (p<0.01), MDA content (p<0.01) and expression levels of cysteinyl 
      aspartate specific proteinase-3 (Caspase-3), NR2B, phosphorylated CaMKIIalpha 
      (p-CaMKIIalpha) and p-cAMP responsive element binding protein (p-CREB) (p<0.01), but 
      it increased the mitochondrial membrane potential (p<0.01) and B-cell lymphoma 2 
      (Bcl-2)/Bcl-2-associated X protein (Bax) ratio (p<0.01). Remifentanil can repress 
      the NR2B/ CaMKIIalpha signaling pathway in the neurons of rats with cerebral IRI to 
      decrease the p-CREB expression, ROS level and MDA content in neurons, neuronal 
      apoptosis level and cerebral infarct size, and increase the mitochondrial 
      membrane potential, thereby protecting the neurological function.
CI  - Copyright 2020 Biolife Sas. www.biolifesas.org.
FAU - Chen, C R
AU  - Chen CR
AD  - Department of Anesthesiology, Xingtai People's Hospital, Xingtai, China.
FAU - Bi, H L
AU  - Bi HL
AD  - Department of Operation room, Affiliated Jinxiang Hospital of Jining Medical 
      University, Jining, China.
FAU - Li, X
AU  - Li X
AD  - Department of Anesthesiology, Affiliated Jinxiang Hospital of Jining Medical 
      University, Jining, China.
FAU - Li, Z M
AU  - Li ZM
AD  - Department of Health Management, The PLA Rocket Force Characteristic Medical 
      Center, Beijing, China.
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Camk2a protein, rat)
RN  - P10582JYYK (Remifentanil)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate
MH  - Remifentanil/pharmacology
MH  - *Reperfusion Injury/prevention & control
MH  - Signal Transduction
OTO - NOTNLM
OT  - Aremifentanil
OT  - NR2B/CaMKIIa signaling pathway
OT  - apoptosis
OT  - cerebral ischemia-reperfusion injury
OT  - rats
EDAT- 2020/10/27 06:00
MHDA- 2021/01/15 06:00
CRDT- 2020/10/26 08:02
PHST- 2020/10/26 08:02 [entrez]
PHST- 2020/10/27 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
AID - 12 [pii]
AID - 10.23812/20-169-A [doi]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2020 Sep-Oct,;34(5):1647-1656. doi: 
      10.23812/20-169-A.