PMID- 33104141
OWN - NLM
STAT- MEDLINE
DCOM- 20210517
LR  - 20210517
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 11
IP  - 11
DP  - 2020 Nov 18
TI  - IRS-1/PI3K/Akt pathway and miRNAs are involved in whole grain highland barley 
      (Hordeum vulgare L.) ameliorating hyperglycemia of db/db mice.
PG  - 9535-9546
LID - 10.1039/d0fo01990a [doi]
AB  - The effects of whole grain highland barley (WGH) with rich phenolics on glucose 
      metabolism, the insulin pathway, and microRNA (miRNA) expression in db/db mice 
      were explored in the present study. Supplementation with WGH decreased the levels 
      of blood glucose, glycosylated serum protein (GSP), insulin, and inflammatory 
      cytokines in db/db mice. Furthermore, WGH administration triggered a remarkable 
      amelioration of glucose intolerance and insulin resistance. The hepatic 
      glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxylase (PEPCK) 
      activities and the G6PC, PEPCK, and forkhead transcription factor 1 (FOXO1) mRNA 
      levels in the WGH-treated group were also reduced. Moreover, WGH promoted the 
      glycogen storage in the liver via up-regulating the activities of hexokinase (HK) 
      and glycogen synthase (GS) and the phosphorylation of glycogen synthase kinase 3beta 
      (GSK3beta) protein, while down-regulating the GSK3beta mRNA level. The protein 
      expression of phosphatidylinositol 3-kinase (PI3K), the phosphorylation of 
      protein kinase B (Akt), and the mRNA levels of insulin receptor substrate-1 
      (IRS-1), PI3K and Akt were also up-regulated by WGH treatment. Moreover, WGH 
      significantly augmented the expression of miRNA-26a and miRNA-451, but reduced 
      those of miRNA-126a and miRNA-29a. These results demonstrated that WGH exhibits a 
      hypoglycemic effect through regulating the IRS-1/PI3K/Akt pathway and related 
      miRNAs, further modulating the expression of G6PC, PEPCK, and FOXO1 mRNAs and 
      p-GSK3beta protein, thus inhibiting hepatic gluconeogenesis, improving glycogen 
      synthesis and alleviating insulin resistance. Therefore, this study suggested WGH 
      as an effective candidate to ameliorate the hyperglycemia of type 2 diabetes 
      mellitus.
FAU - Deng, Na
AU  - Deng N
AD  - Overseas Expertise Introduction Center for Discipline Innovation of Food 
      Nutrition and Human Health (111 Center), School of Food Science and Engineering, 
      South China University of Technology, Guangzhou, 510641, China. 
      febzheng@scut.edu.cn.
FAU - Guo, Ruixue
AU  - Guo R
FAU - Zheng, Bisheng
AU  - Zheng B
FAU - Li, Tong
AU  - Li T
FAU - Liu, Rui Hai
AU  - Liu RH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Type 2/*prevention & control
MH  - Disease Models, Animal
MH  - *Functional Food
MH  - *Hordeum
MH  - Hyperglycemia/*prevention & control
MH  - Hypoglycemic Agents/administration & dosage/pharmacology/*therapeutic use
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Obese
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction/drug effects
EDAT- 2020/10/27 06:00
MHDA- 2021/05/18 06:00
CRDT- 2020/10/26 12:10
PHST- 2020/10/27 06:00 [pubmed]
PHST- 2021/05/18 06:00 [medline]
PHST- 2020/10/26 12:10 [entrez]
AID - 10.1039/d0fo01990a [doi]
PST - ppublish
SO  - Food Funct. 2020 Nov 18;11(11):9535-9546. doi: 10.1039/d0fo01990a.