PMID- 33105725
OWN - NLM
STAT- MEDLINE
DCOM- 20210413
LR  - 20210413
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 21
DP  - 2020 Oct 22
TI  - Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem 
      Cells to Suppress T-Cells.
LID - 10.3390/ijms21217831 [doi]
LID - 7831
AB  - Retinal ganglion cells (RGCs) are impaired in patients such as those with 
      glaucoma and optic neuritis, resulting in permanent vision loss. To restore 
      visual function, development of RGC transplantation therapy is now underway. 
      Induced pluripotent stem cells (iPSCs) are an important source of RGCs for human 
      allogeneic transplantation. We therefore analyzed the immunological 
      characteristics of iPSC-derived RGCs (iPSC-RGCs) to evaluate the possibility of 
      rejection after RGC transplantation. We first assessed the expression of human 
      leukocyte antigen (HLA) molecules on iPSC-RGCs using immunostaining, and then 
      evaluated the effects of iPSC-RGCs to activate lymphocytes using the mixed 
      lymphocyte reaction (MLR) and iPSC-RGC co-cultures. We observed low expression of 
      HLA class I and no expression of HLA class II molecules on iPSC-RGCs. We also 
      found that iPSC-RGCs strongly suppressed various inflammatory immune cells 
      including activated T-cells in the MLR assay and that transforming growth 
      factor-beta2 produced by iPSC-RGCs played a critical role in suppression of 
      inflammatory cells in vitro. Our data suggest that iPSC-RGCs have low 
      immunogenicity, and immunosuppressive capacity on lymphocytes. Our study will 
      contribute to predicting immune attacks after RGC transplantation.
FAU - Edo, Ayaka
AU  - Edo A
AUID- ORCID: 0000-0001-7701-605X
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
AD  - Department of Ophthalmology and Visual Science, Graduate School of Biomedical and 
      Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 
      734-8551, Japan.
FAU - Sugita, Sunao
AU  - Sugita S
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Futatsugi, Yoko
AU  - Futatsugi Y
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Sho, Junki
AU  - Sho J
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Onishi, Akishi
AU  - Onishi A
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
FAU - Kiuchi, Yoshiaki
AU  - Kiuchi Y
AD  - Department of Ophthalmology and Visual Science, Graduate School of Biomedical and 
      Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 
      734-8551, Japan.
FAU - Takahashi, Masayo
AU  - Takahashi M
AD  - Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics 
      Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
LA  - eng
GR  - JP18bm0204002, JP17bk0104002/Japan Agency for Medical Research and Development/
PT  - Journal Article
DEP - 20201022
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (HLA Antigens)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Graft Rejection
MH  - HLA Antigens/genetics/immunology/metabolism
MH  - Humans
MH  - Immune Tolerance
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Retinal Ganglion Cells/*cytology/*immunology/transplantation
MH  - T-Lymphocytes/*immunology
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC7660053
OTO - NOTNLM
OT  - immunogenicity
OT  - immunosuppression
OT  - induced pluripotent stem cells
OT  - mixed lymphocyte reaction
OT  - retinal ganglion cells
COIS- The authors declare no competing interests.
EDAT- 2020/10/28 06:00
MHDA- 2021/04/14 06:00
PMCR- 2020/11/01
CRDT- 2020/10/27 01:02
PHST- 2020/09/18 00:00 [received]
PHST- 2020/10/08 00:00 [revised]
PHST- 2020/10/20 00:00 [accepted]
PHST- 2020/10/27 01:02 [entrez]
PHST- 2020/10/28 06:00 [pubmed]
PHST- 2021/04/14 06:00 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - ijms21217831 [pii]
AID - ijms-21-07831 [pii]
AID - 10.3390/ijms21217831 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Oct 22;21(21):7831. doi: 10.3390/ijms21217831.