PMID- 33107268
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 34
IP  - 5
DP  - 2020 Oct 26
TI  - Resolvin D1 alleviates cerebral ischemia/reperfusion injury in rats by inhibiting 
      NLRP3 signaling pathway.
LID - 10.23812/20-392-A [doi]
AB  - The purpose of the present study was to investigate the role of resolvin D1 
      (RvD1) in cerebral ischemia/ reperfusion (I/R) injury in rats and its mechanism. 
      A total of 60 adult male Wistar rats were divided into 3 groups using a random 
      number table, including sham-operation group (Sham group, n=20), cerebral I/R 
      injury group (I/R group, n=20) and cerebral I/R injury + RvD1 pretreatment group 
      (I/R + RvD1 group, n=20). The model of focal I/R injury was established using 
      suture method through 30 min of ischemia and 24 h of reperfusion. In I/R + RvD1 
      group, the rats were intraperitoneally injected with RvD1 (4 mg/kg/d) at 7 d 
      before operation, while those in the Sham group and I/R group were injected with 
      an equal volume of normal saline. After reperfusion, the area of cerebral 
      infarction was evaluated by means of 2,3,5-triphenyltetrazolium chloride (TTC) 
      staining. Then hematoxylin and eosin (H&E) staining was applied to observe the 
      status of brain tissue injury in each group of rats, and the expression of 
      malondialdehyde (MDA), a marker of oxidative stress, in each group of brain 
      tissues was detected via an oxidative stress detection kit. Moreover, reverse 
      transcription-polymerase chain reaction (RT-PCR) assay was performed to measure 
      the levels of inflammation-related genes [interleukin-1 beta (IL-1beta) and tumor 
      necrosis factor-alpha (TNF-alpha)] in the brain tissues of each group of rats, and 
      the neuronal apoptosis level in the brain tissues in each group was determined 
      through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) assay. Finally, the expression levels of proteins related to the 
      inflammasome signaling pathway were detected via Western blotting assay. It was 
      indicated in the results of TTC staining and H&E staining that RvD1 could 
      remarkably decrease the area of I/R-induced cerebral infarction and relieve 
      nervous tissue injury (P<0.05). The results of TUNEL staining revealed that the 
      cerebral neuronal apoptosis induced by I/R injury was alleviated by RvD1 
      (P<0.05). In addition, RvD1 lowered the levels of inflammatory factors and MDA in 
      the brain tissues of rats with I/R injury (P<0.05). Furthermore, it was 
      discovered that RvD1 repressed the protein expression of nucleotide-binding 
      oligomerization domain-like receptor protein 3 (NLRP3) in the brain tissues of 
      rats with I/R injury (P<0.05). The protective effect of RvD1 on the rats against 
      cerebral I/R injury may be related to its inhibition on NLRP3 inflammasome, and 
      RvD1 is expected to become a targeted drug for the clinical treatment of cerebral 
      I/R injury.
CI  - Copyright 2020 Biolife Sas. www.biolifesas.org.
FAU - Chen, J J
AU  - Chen JJ
AD  - Department of Neuromedicine, The First Hospital of Lanzhou University, Lanzhou, 
      China.
FAU - Chen, J
AU  - Chen J
AD  - Department of Neuromedicine, The First Hospital of Lanzhou University, Lanzhou, 
      China.
FAU - Jiang, Z X
AU  - Jiang ZX
AD  - Department of Neuromedicine, The First Hospital of Lanzhou University, Lanzhou, 
      China.
FAU - Zhou, Z
AU  - Zhou Z
AD  - Department of Neuromedicine, The First Hospital of Lanzhou University, Lanzhou, 
      China.
FAU - Zhou, C N
AU  - Zhou CN
AD  - Department of Neuromedicine, The First Hospital of Lanzhou University, Lanzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20201026
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
SB  - IM
OTO - NOTNLM
OT  - cerebral ischemia-reperfusion injury
OT  - inflammasome
OT  - resolvin D1
EDAT- 2020/10/28 06:00
MHDA- 2020/10/28 06:00
CRDT- 2020/10/27 06:46
PHST- 2020/10/27 06:46 [entrez]
PHST- 2020/10/28 06:00 [pubmed]
PHST- 2020/10/28 06:00 [medline]
AID - 25 [pii]
AID - 10.23812/20-392-A [doi]
PST - aheadofprint
SO  - J Biol Regul Homeost Agents. 2020 Oct 26;34(5). doi: 10.23812/20-392-A.