PMID- 33107868
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 146
IP  - 1
DP  - 2021 Jan 4
TI  - A label-free photoelectrochemical immunosensor for carcinoembryonic antigen 
      detection based on a g-C(3)N(4)/CdSe nanocomposite.
PG  - 146-155
LID - 10.1039/d0an01656j [doi]
AB  - Herein, a label-free photoelectrochemical immunosensor based on a g-C3N4/CdSe 
      nanocomposite was established and applied to detect carcinoembryonic antigen 
      (CEA). The prepared nanocomposite materials were characterized by transmission 
      electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible 
      absorption spectroscopy (UV-vis), X-ray photoelectron spectroscopy (XPS), fourier 
      transform infrared spectrometer (FT-IR) and photoluminescence spectroscopy (PL). 
      The results indicate that g-C3N4/CdSe nanocomposite materials were successfully 
      synthesized. In a typical assembly process, the immunosensor was constructed by 
      modifying a fluorine-doped tin oxide (FTO) electrode with poly dimethyl diallyl 
      ammonium chloride (PDDA), the g-C3N4/CdSe nanocomposite, the 
      anti-carcinoembryonic antigen antibody (Ab) and the blocking agent bovine serum 
      albumin (BSA) successively. In the presence of CEA, the photocurrent signal of 
      the prepared immunosensor decreased significantly. Accordingly, under the optimal 
      conditions, a label-free photoelectrochemical immunosensor was established, and 
      it exhibited excellent selectivity and repeatability for CEA detection. The 
      detection limit was 0.21 ng mL-1, and the range was 10 ng mL-1-100 mug mL-1. 
      Simultaneously, the immunosensor also provides a likely sensing device for 
      detecting other protein targets, which is of great significance for early 
      clinical diagnosis.
FAU - Liu, Xing-Pei
AU  - Liu XP
AD  - Key Laboratory of Structure and Functional Regulation of Hybrid Materials 
      (Ministry of Education), Key Laboratory of Chemistry for Inorganic/Organic Hybrid 
      Functionalized Materials of Anhui Province, Key Laboratory of Functional 
      Inorganic Materials of Anhui Province, School of Chemistry & Chemical 
      Engineering, Anhui University, Hefei, 230601, PR China. maochangjie@sina.com 
      cjshuai@126.com.
FAU - Chen, Jing-Shuai
AU  - Chen JS
FAU - Mao, Chang-Jie
AU  - Mao CJ
FAU - Jin, Bao-Kang
AU  - Jin BK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Cadmium Compounds)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Selenium Compounds)
SB  - IM
MH  - *Biosensing Techniques
MH  - *Cadmium Compounds
MH  - Carcinoembryonic Antigen
MH  - Electrochemical Techniques
MH  - Immunoassay
MH  - *Nanocomposites
MH  - *Selenium Compounds
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2020/10/28 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/10/27 12:13
PHST- 2020/10/28 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/10/27 12:13 [entrez]
AID - 10.1039/d0an01656j [doi]
PST - ppublish
SO  - Analyst. 2021 Jan 4;146(1):146-155. doi: 10.1039/d0an01656j.