PMID- 33107987
OWN - NLM
STAT- MEDLINE
DCOM- 20210407
LR  - 20210407
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 131
IP  - 4
DP  - 2021 Apr
TI  - Prognostic Impact of Tumor-Infiltrating Lymphocytes in Laryngeal Squamous Cell 
      Carcinoma Patients.
PG  - E1249-E1255
LID - 10.1002/lary.29196 [doi]
AB  - OBJECTIVES/HYPOTHESIS: Tumor-infiltrating lymphocytes (TILs) has been shown to be 
      associated with the prognosis of many tumors, yet few studies have investigated 
      their roles in laryngeal squamous cell carcinoma (LSCC). We aim to investigate 
      the prognostic values of tumor-infiltrating CD3(+) /CD4(+) /CD8(+) /Foxp3(+) 
      T-cells and neutrophils in LSCC patients that received total or partial 
      laryngectomy. STUDY DESIGN: Retrospective case series of LSCC patients who 
      underwent total or partial laryngectomy from 2013 to 2014 at Eye, Ear, Nose, and 
      Throat Hospital of Fudan University. METHODS: In our study, 41 tumor tissues from 
      patients with LSCC were retrospectively assessed using immunohistochemistry for 
      CD3(+) /CD4(+) /CD8(+) /Foxp3(+) T-cells and CD66b(+) neutrophils. Overall 
      survival (OS) and disease-free survival (DFS) were recorded using Kaplan-Meier 
      methods. RESULTS: Generally, patients with high density of TILs (CD3, CD4, CD8) 
      showed improved OS or DFS. Specifically, high density of CD3(+) TILs were 
      associated with better OS, yet poorer OS and DFS for CD66b(+) neutrophils. 
      Patients with an Immunoscore of 0-1 experienced the worst OS and DFS, compared 
      with Immunoscore 2-4 (P = .0111 for OS, P = .0391 for DFS). In Cox proportional 
      hazards analysis adjusted for N stage and T stage, only stroma CD66b(+) 
      neutrophils densities were able to predict OS, with odds ratios of 4.819 (95% 
      confidence interval [CI] 1.149-20.206; P = .032*), and DFS 2.888 (95% CI 
      1.043-7.997; P = .041*). CONCLUSIONS: The density of TILs and CD66b(+) 
      neutrophils may help predict the prognosis of patients with LSCC after surgery. 
      LEVEL OF EVIDENCE: 3 Laryngoscope, 131:E1249-E1255, 2021.
CI  - (c) 2020 American Laryngological, Rhinological and Otological Society Inc, "The 
      Triological Society" and American Laryngological Association (ALA).
FAU - Zhang, Duo
AU  - Zhang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Tang, Di
AU  - Tang D
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Heng, Yu
AU  - Heng Y
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Zhu, Xiao-Ke
AU  - Zhu XK
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Tao, Lei
AU  - Tao L
AUID- ORCID: 0000-0003-3384-9396
AD  - Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai 
      Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of 
      Medicine, Shanghai, People's Republic of China.
AD  - Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, 
      People's Republic of China.
FAU - Lu, Li-Ming
AU  - Lu LM
AD  - Shanghai Institute of Immunology, Shanghai Jiaotong University School of 
      Medicine, Shanghai, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201027
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*immunology/mortality/pathology/surgery
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*immunology/mortality/pathology/surgery
MH  - Laryngectomy
MH  - *Lymphocytes, Tumor-Infiltrating
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Neutrophil Infiltration
MH  - Prognosis
MH  - Retrospective Studies
MH  - Survival Rate
OTO - NOTNLM
OT  - CD3
OT  - CD66b
OT  - CD8
OT  - Tumor-infiltrating lymphocytes
OT  - laryngeal squamous cell carcinoma
EDAT- 2020/10/28 06:00
MHDA- 2021/04/10 06:00
CRDT- 2020/10/27 12:14
PHST- 2020/02/19 00:00 [received]
PHST- 2020/09/13 00:00 [revised]
PHST- 2020/10/08 00:00 [accepted]
PHST- 2020/10/28 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2020/10/27 12:14 [entrez]
AID - 10.1002/lary.29196 [doi]
PST - ppublish
SO  - Laryngoscope. 2021 Apr;131(4):E1249-E1255. doi: 10.1002/lary.29196. Epub 2020 Oct 
      27.