PMID- 33108039 OWN - NLM STAT- MEDLINE DCOM- 20210607 LR - 20210607 IS - 1861-471X (Electronic) IS - 1861-471X (Linking) VI - 15 IP - 24 DP - 2020 Dec 14 TI - Influence of Nitrogen to Phosphorus Ratio and CO(2) Concentration on Lipids Accumulation of Scenedesmus dimorphus for Bioenergy Production and CO(2) Biofixation. PG - 4307-4320 LID - 10.1002/asia.202001063 [doi] AB - The potential of Scenedesmus dimorphus microalgae for CO(2) biofixation and lipid biosynthesis for bioenergy applications was evaluated in this study. Batch experiments were conducted using synthetic tertiary municipal wastewater samples at several nitrogen to phosphorus (NP) ratios (1 : 1 to 8 : 1) and CO(2) concentrations ( approximately 0%, 2%, 4%, and 6% CO(2) in supplied air). Scenedesmus dimorphus was cultivated for 25 days and the growth is highly dependent on the CO(2) concentration and the NP ratio. An NP ratio of 2 : 1 produces a biomass yield of 733 mg/L when the microalga culture was supplied with air enriched with 2% CO(2) . The maximum CO(2) biofixation rate of 49.6 mg L(-1) d(-1) is at an NP ratio of 8 : 1 with 4% CO(2) . A colorimetric technique depending on sulpho-phospho-vanillin (SPV) was utilized for the determination of the intracellular lipid content. The highest lipid content of 31.6% as the dry weight of the biomass is at an NP ratio of 1 : 1 and 6% CO(2) . These results indicate that supplementation of suitable CO(2) with favorable NP ratio has a considerable effect on lipid accumulation in the microalgae Scenedesmus dimorphus biomass. CI - (c) 2020 Wiley-VCH GmbH. FAU - Omar Faruque, Mohammed AU - Omar Faruque M AD - Chemical Engineering Department, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia. FAU - Ilyas, Muhammad AU - Ilyas M AD - Center for Environment and Water, RI, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia. FAU - Mozahar Hossain, Mohammad AU - Mozahar Hossain M AD - Chemical Engineering Department, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia. FAU - Abdur Razzak, Shaikh AU - Abdur Razzak S AUID- ORCID: 0000-0003-4316-7882 AD - Chemical Engineering Department, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia. LA - eng GR - DF191050/King Fahd University of Petroleum & Minerals/ PT - Journal Article DEP - 20201116 PL - Germany TA - Chem Asian J JT - Chemistry, an Asian journal JID - 101294643 RN - 0 (Benzaldehydes) RN - 0 (Biofuels) RN - 0 (Lipids) RN - 142M471B3J (Carbon Dioxide) RN - 27YLU75U4W (Phosphorus) RN - CHI530446X (vanillin) RN - N762921K75 (Nitrogen) SB - IM MH - Benzaldehydes/chemistry MH - *Biofuels MH - Biomass MH - Carbon Dioxide/chemistry/*metabolism MH - Colorimetry MH - Lipids/analysis/*biosynthesis MH - Nitrogen/*chemistry MH - Phosphorus/*chemistry MH - Scenedesmus/growth & development/*metabolism MH - Waste Disposal, Fluid OTO - NOTNLM OT - Algal growth OT - CO2 Biofixation OT - Lipid accumulation OT - Microalgae biomass OT - Tertiary municipal wastewater EDAT- 2020/10/28 06:00 MHDA- 2021/06/08 06:00 CRDT- 2020/10/27 12:15 PHST- 2020/09/06 00:00 [received] PHST- 2020/10/26 00:00 [revised] PHST- 2020/10/28 06:00 [pubmed] PHST- 2021/06/08 06:00 [medline] PHST- 2020/10/27 12:15 [entrez] AID - 10.1002/asia.202001063 [doi] PST - ppublish SO - Chem Asian J. 2020 Dec 14;15(24):4307-4320. doi: 10.1002/asia.202001063. Epub 2020 Nov 16.