PMID- 33113607
OWN - NLM
STAT- MEDLINE
DCOM- 20210112
LR  - 20210112
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 41
IP  - 9
DP  - 2020 Sep 14
TI  - [Diagnosis of adult Philadelphia chromosome-like acute lymphoblastic leukemia by 
      fluorescence in situ hybridization].
PG  - 749-755
LID - 10.3760/cma.j.issn.0253-2727.2020.09.008 [doi]
AB  - Objective: To establish a screening system of adult Philadelphia chromosome-like 
      acute lymphoblastic leukemia (Ph-like ALL) by fluorescence in situ hybridization 
      (FISH) . Method: Based on the genetic characteristics of Ph-like ALL, FISH probes 
      were designed for ABL1, ABL2, JAK2, EPOR, CRLF2, CSF1R, PDGFRB, and P2RY8 gene 
      breakpoints, which were used to screen Ph-like ALL in B-ALL patients without 
      BCR-ABL1, ETV6-RUNX1, MLL, and E2A gene arrangement. Furthermore, it was analyzed 
      in combination with flow immunophenotype, next-generation sequencing for targeted 
      gene mutations, and RNA sequencing (RNA-seq) . Results: A total of 189 adult 
      B-ALL patients diagnosed in Nanfang Hospital from January 2016 to April 2019 were 
      enrolled in this study. Using FISH and/or PCR, BCR-ABL1, ETV6-RUNX1, MLL, or E2A 
      arrangement was detected in 83 of them, and Ph-like ALL was detected by FISH in 
      the other 106, resulting in the presence of typical gene arrangements of Ph-like 
      ALL in 12 patients (11.3% , 12/106) . Validated by RNA-seq, the sensitivity and 
      specificity of FISH for Ph-like ALL were 71.4% and 95.8% , respectively. After 
      further analysis with immunophenotype, targeted gene mutations, and RNA-seq, 14 
      (13.2% , 14/106) were diagnosed with Ph-like ALL. Conclusion: This data shows 
      high specificity of FISH for identification of Ph-like ALL and combining 
      immunophenotype and sequencing technology can improve the diagnostic system.
FAU - Lin, D N
AU  - Lin DN
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Li, Q L
AU  - Li QL
AD  - Department of Hematology, the People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530000, China.
FAU - He, X J
AU  - He XJ
AD  - Department of Hematology, General Hospital of Southern Theatre Command of PLA, 
      Guangzhou, 510010, China.
FAU - Li, H
AU  - Li H
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Liao, L B
AU  - Liao LB
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - He, H
AU  - He H
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Zhou, L L
AU  - Zhou LL
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Li, Z
AU  - Li Z
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Liu, X L
AU  - Liu XL
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Liu, Q F
AU  - Liu QF
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Zhou, H S
AU  - Zhou HS
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Cao, R
AU  - Cao R
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, 510515, China.
LA  - chi
GR  - A2017307/Foundation for Program of Medical Science and Technology of Guangdong 
      Province/
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Philadelphia Chromosome
MH  - *Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/diagnosis
PMC - PMC7595857
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Leukemia, lymphoblastic, acute
OT  - Philadelphia chromosome-like
EDAT- 2020/10/29 06:00
MHDA- 2021/01/13 06:00
PMCR- 2020/09/01
CRDT- 2020/10/28 23:15
PHST- 2020/10/28 23:15 [entrez]
PHST- 2020/10/29 06:00 [pubmed]
PHST- 2021/01/13 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - cjh-41-09-749 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2020.09.008 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2020 Sep 14;41(9):749-755. doi: 
      10.3760/cma.j.issn.0253-2727.2020.09.008.