PMID- 33113626
OWN - NLM
STAT- MEDLINE
DCOM- 20210108
LR  - 20221207
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 42
IP  - 10
DP  - 2020 Oct 23
TI  - [Deleted in lymphocytic leukemia 1 promoted proliferation and apoptosis of 
      nephroblastoma cells through regulating miR-513a-5p and RANBP2 pathway].
PG  - 849-855
LID - 10.3760/cma.j.cn112152-20200311-00194 [doi]
AB  - Objective: To study the regulatory effects and mechanisms of deleted in 
      lymphocytic leukemia 1 (DLEU1), microRNA-513a-5p (miR-513a-5p), and RAN binding 
      protein 2 (RANBP2) in nephroblastoma. Methods: The GHINK-1 cells were transfected 
      with pcDNA (pcDNA group), pcDNA-DLEU1 (pcDNA-DLEU1 group), miR-NC (miR-NC group), 
      miR-513a-5p mimics (miR-513a-5p group), pcDNA-RANBP2 (pcDNA-RANBP2 group), 
      pcDNA-DLEU1 and miR-NC (pcDNA-DLEU1+ miR-NC group), pcDNA-DLEU1 and miR-513a-5p 
      mimics (pcDNA-DLEU1+ miR-513a-5p group), miR-513a-5p mimics and pcDNA 
      (miR-513a-5p+ pcDNA group), miR-513a-5p mimics and pcDNA-RANBP2 (miR-513a-5p + 
      pcDNA-RANBP2 group). Real-time quantitative reverse transcription polymerase 
      chain reaction (RT-qPCR) was used to detect the expressions of DLEU1, 
      miR-513a-5p, RANBP2 in nephroblastoma tissues, normal adjacent tissues, normal 
      kidney cell HK2, and hemangioblastoma cell GHINK-1. Western blot was used to 
      detect the expressions of proliferating cell nuclear antigen (PCNA), B cell 
      lymphoma/leukemia-2 (Bcl-2) and Bcl-2 related X (Bax). Cell counting kit 8 
      (CCK-8) was used to detect the cell survival rate. Flow cytometry was used to 
      detect the apoptosis rate. Dual luciferase report test was used to detect the 
      luciferase activity of cells. Results: The expression levels of DLEU1, 
      miR-513a-5p and RANBP2 in adjacent tissues were 1.02+/-0.08, 1.01+/-0.06, 1.00+/-0.05, 
      respectively, significantly lower than 5.16+/-0.24, 0.23+/-0.02, 1.67+/-0.09 in 
      nephroblasts tumor tissues (P<0.05). Their expression levels in HK2 cells were 
      1.00+/-0.06, 1.00+/-0.08, 1.02+/-0.09, respectively, significantly lower than 
      3.15+/-0.21, 0.18+/-0.01, 1.54+/-0.10 in GHINK-1 cells (P<0.05). Overexpression of 
      DLEU1 significantly reduced the apoptosis rate (7.35+/-0.41 vs 12.35+/-1.12, P<0.05). 
      Overexpression of RANBP2 significantly reduced the apoptosis rate (8.89+/-0.48 vs 
      12.64+/-1.12, P<0.05). Compared with the miR-NC group (1.01+/-0.06, 0.99+/-0.06), the 
      luciferase activity of DLEU1-WT (0.43+/-0.04) and RANBP2-WT (0.61+/-0.07) in 
      miR-513a-5p group were significantly reduced (P<0.05). Compared with anti-miR-NC 
      group (0.99+/-0.07, 0.98+/-0.05), the luciferase activity of DLEU1-WT (1.34+/-0.11) and 
      RANBP2-WT (1.39 +/-0.13) in anti-miR-513a-5p group was significantly increased 
      (P<0.05). Simultaneous overexpression of pcDNA-DLEU1 and miR-513a-5p in GHINK-1 
      cells significantly reduced the apoptosis rate (11.34+/-1.03 vs 8.51+/-0.69, P<0.05). 
      Simultaneous overexpression of miR-513a-5p and RANBP2 in GHINK-1 cells 
      significantly reduced the apoptosis rate (9.96+/-0.72 vs 15.94+/-1.00, P<0.05). 
      Conclusions: The long-chain non-coding RNA (lncRNA) DLEU1 can promote the 
      proliferation and inhibit the apoptosis of nephroblastoma cells. The mechanism is 
      related to the targeted regulation of miR-513a-5p and RANBP2 function, which will 
      provide theoretical support for the nephroblastoma treatment.
FAU - Zhao, J L
AU  - Zhao JL
AD  - Department of Pediatrics, Nanyang Central Hospital, Nanyang 473000, China.
FAU - Zhao, L L
AU  - Zhao LL
AD  - Department of Pediatrics, Nanyang Central Hospital, Nanyang 473000, China.
FAU - Niu, W Z
AU  - Niu WZ
AD  - Department of Pediatrics, Nanyang Central Hospital, Nanyang 473000, China.
FAU - Ding, X C
AU  - Ding XC
AD  - Department of Pediatrics, Nanyang Central Hospital, Nanyang 473000, China.
FAU - Zhang, W L
AU  - Zhang WL
AD  - Department of Children Hematology and Oncology, Henan Cancer Hospital, Zhengzhou 
      450000, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (DLEU1 lncRNA, human)
RN  - 0 (MIRN513A1 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (ran-binding protein 2)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Molecular Chaperones/*genetics
MH  - Nuclear Pore Complex Proteins/*genetics
MH  - RNA, Long Noncoding
MH  - Tumor Suppressor Proteins/*genetics
MH  - *Wilms Tumor/genetics
OTO - NOTNLM
OT  - Deleted in lymphocytic leukemia 1
OT  - MiR-513a-5p
OT  - Nephroblastoma
OT  - RANBP2
EDAT- 2020/10/29 06:00
MHDA- 2021/01/09 06:00
CRDT- 2020/10/28 23:15
PHST- 2020/10/28 23:15 [entrez]
PHST- 2020/10/29 06:00 [pubmed]
PHST- 2021/01/09 06:00 [medline]
AID - 10.3760/cma.j.cn112152-20200311-00194 [doi]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2020 Oct 23;42(10):849-855. doi: 
      10.3760/cma.j.cn112152-20200311-00194.