PMID- 33116165
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20231107
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Oct 28
TI  - Modelling early events in Mycobacterium bovis infection using a co-culture model 
      of the bovine alveolus.
PG  - 18495
LID - 10.1038/s41598-020-75113-6 [doi]
LID - 18495
AB  - Bovine tuberculosis (bTB), a zoonosis mainly caused by Mycobacterium bovis has 
      severe socio-economic consequences and impact on animal health. Host-pathogen 
      interactions during M. bovis infection are poorly understood, especially early 
      events which are difficult to follow in vivo. This study describes the 
      utilisation of an in vitro co-culture model, comprising immortalised bovine 
      alveolar type II (BATII) epithelial cells and bovine pulmonary arterial 
      endothelial cells (BPAECs). When cultured at air-liquid interface, it was 
      possible to follow the migration of live M. bovis Bacille Calmette-Guerin (BCG) 
      and to observe interactions with each cell type, alongside cytokine release. 
      Infection with BCG was shown to exert a detrimental effect primarily upon 
      epithelial cells, with corresponding increases in IL8, TNFalpha, IL22 and IL17a 
      cytokine release, quantified by ELISA. BCG infection increased expression of 
      CD54, MHC Class I and II molecules in endothelial but not epithelial cells, which 
      exhibited constitutive expression. The effect of peripheral blood mononuclear 
      cell conditioned medium from vaccinated cattle upon apical-basolateral migration 
      of BCG was examined by quantifying recovered BCG from the apical, membrane and 
      basolateral fractions over time. The numbers of recovered BCG in each fraction 
      were unaffected by the presence of PBMC conditioned medium, with no observable 
      differences between vaccinated and naive animals.
FAU - Lee, Diane Frances
AU  - Lee DF
AD  - School of Veterinary Medicine, University of Surrey, Guildford, Surrey, UK. 
      diane.lee@surrey.ac.uk.
FAU - Stewart, Graham Roger
AU  - Stewart GR
AD  - School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, UK.
FAU - Chambers, Mark Andrew
AU  - Chambers MA
AD  - School of Veterinary Medicine, University of Surrey, Guildford, Surrey, UK.
AD  - School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201028
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (BCG Vaccine)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
SB  - IM
MH  - Alveolar Epithelial Cells/*microbiology
MH  - Animals
MH  - Apoptosis
MH  - *BCG Vaccine
MH  - Cattle
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Cytokines/metabolism
MH  - Endothelial Cells/*microbiology
MH  - Inflammation
MH  - Leukocytes, Mononuclear/cytology
MH  - Lung Diseases/*microbiology
MH  - Mycobacterium bovis/pathogenicity
MH  - Necrosis
MH  - Pulmonary Alveoli/*cytology
MH  - Tuberculosis, Bovine/metabolism/*microbiology
MH  - Up-Regulation
MH  - Vaccination/veterinary
PMC - PMC7595104
COIS- The authors declare no competing interests.
EDAT- 2020/10/30 06:00
MHDA- 2021/03/03 06:00
PMCR- 2020/10/28
CRDT- 2020/10/29 05:44
PHST- 2020/06/26 00:00 [received]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/10/29 05:44 [entrez]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2020/10/28 00:00 [pmc-release]
AID - 10.1038/s41598-020-75113-6 [pii]
AID - 75113 [pii]
AID - 10.1038/s41598-020-75113-6 [doi]
PST - epublish
SO  - Sci Rep. 2020 Oct 28;10(1):18495. doi: 10.1038/s41598-020-75113-6.