PMID- 33116722 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220417 IS - 1178-7007 (Print) IS - 1178-7007 (Electronic) IS - 1178-7007 (Linking) VI - 13 DP - 2020 TI - Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes. PG - 3727-3737 LID - 10.2147/DMSO.S273586 [doi] AB - BACKGROUND: Type 2 diabetes mellitus [T2DM] has been one of the common diseases and is characterized by increased blood glucose levels and suggested that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in diabetes. MATERIALS/METHODS: The present study included clinically confirmed newly diagnosed 200 cases of T2DM and 200 healthy subjects, and all the parameters were taken care in diagnosis. Blood samples collected in plain vials were used for cell-free total RNA extraction and after that 100ng of total RNA was used to synthesize the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls. RESULTS: It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p<0.0001], weight loss [p=0.02] and fatigue [p=0.02]. Increased miRNA29a expression was linked with hypertension [p<0.0001], alcoholism [p=0.04], and smoking [p<0.0001] as well as miRNA-29b expression was associated with hypertension [p=0.0001], weight loss [p=0.002], smoking [p=0.0002], alcoholism [p<0.0001]. Low [1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p<0.0001] expression. lncRNA H19 expression showed negative correlation with miRNA-29a expression [r= -27, p<0.0001] and miRNA-29b [r= -47, p<0.0001]. CONCLUSION: The present study concluded that lower lncRNA H19 expression, and increased miRNA-29b, a miRNA-29b expression associated with the severity of T2DM patients. Decreased lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression observed to be interrelated with clinicopathological findings of T2DM patients could involve in pathogenesis disease. CI - (c) 2020 Alfaifi et al. FAU - Alfaifi, Mohammed AU - Alfaifi M AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. FAU - Verma, Amit Kumar AU - Verma AK AD - Department of Zoology and Environmental Sciences, GKV, Haridwar, India. FAU - Alshahrani, Mohammad Yahya AU - Alshahrani MY AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. FAU - Joshi, Prakash C AU - Joshi PC AUID- ORCID: 0000-0001-6762-8327 AD - Department of Zoology and Environmental Sciences, GKV, Haridwar, India. FAU - Alkhathami, Ali G AU - Alkhathami AG AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. FAU - Ahmad, Irfan AU - Ahmad I AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. FAU - Hakami, Abdulrahim Refdan AU - Hakami AR AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. FAU - Beg, Mirza Masroor Ali AU - Beg MMA AUID- ORCID: 0000-0002-4519-721X AD - Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India. AD - Department of Biochemistry, Maulana Azad Medical College, New Delhi, India. LA - eng PT - Journal Article DEP - 20201014 PL - New Zealand TA - Diabetes Metab Syndr Obes JT - Diabetes, metabolic syndrome and obesity : targets and therapy JID - 101515585 PMC - PMC7569053 OTO - NOTNLM OT - cell-free system OT - diabetes OT - gene expression OT - lncRNA OT - microRNA COIS- The authors report no conflicts of interest for this work. EDAT- 2020/10/30 06:00 MHDA- 2020/10/30 06:01 PMCR- 2020/10/14 CRDT- 2020/10/29 05:49 PHST- 2020/07/23 00:00 [received] PHST- 2020/09/08 00:00 [accepted] PHST- 2020/10/29 05:49 [entrez] PHST- 2020/10/30 06:00 [pubmed] PHST- 2020/10/30 06:01 [medline] PHST- 2020/10/14 00:00 [pmc-release] AID - 273586 [pii] AID - 10.2147/DMSO.S273586 [doi] PST - epublish SO - Diabetes Metab Syndr Obes. 2020 Oct 14;13:3727-3737. doi: 10.2147/DMSO.S273586. eCollection 2020.