PMID- 33117816
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201030
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 8
DP  - 2020
TI  - NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and 
      Acidification Against Vibrio vulnificus Infection.
PG  - 587961
LID - 10.3389/fcell.2020.587961 [doi]
LID - 587961
AB  - The marine bacterium Vibrio vulnificus causes potentially fatal bloodstream 
      infections, typically in patients with chronic liver diseases. The inflammatory 
      response and anti-bacterial function of phagocytes are crucial for limiting 
      bacterial infection in the human hosts. How V. vulnificus affects macrophages 
      after phagocytosis is unclear. In this report, we found that the bactericidal 
      activity of macrophages to internalize V. vulnificus was dependent on mammalian 
      target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain 
      containing 3 (NLRP3) interaction. Additionally, the NLRP3 expression was 
      dependent on mTORC1 activation. Inhibited mTORC1 or absence of NLRP3 in 
      macrophages impaired V. vulnificus-induced phagosome acidification and 
      phagolysosome formation, leading to a reduction of intracellular bacterial 
      clearance. mTORC1 signaling overactivation could increase NLRP3 expression and 
      restore insufficient phagosome acidification. Together, these findings indicate 
      that the intracellular bactericidal activity of macrophages responding to V. 
      vulnificus infection is tightly controlled by the crosstalk of NLRP3 and mTOR and 
      provide critical insight into the host bactericidal activity basis of clearance 
      of V. vulnificus through lyso/phagosome.
CI  - Copyright (c) 2020 Huang, Ma, Zheng, Chen, Hu, Wu, Zheng, Lou and Xie.
FAU - Huang, Xian-Hui
AU  - Huang XH
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
AD  - Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.
FAU - Ma, Yao
AU  - Ma Y
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
FAU - Zheng, Meng-Meng
AU  - Zheng MM
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
FAU - Chen, Na
AU  - Chen N
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
FAU - Hu, Mei-Na
AU  - Hu MN
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
FAU - Wu, Liu-Ying
AU  - Wu LY
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
FAU - Zheng, Yi
AU  - Zheng Y
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
AD  - Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.
FAU - Lou, Yong-Liang
AU  - Lou YL
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
AD  - Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.
FAU - Xie, Dan-Li
AU  - Xie DL
AD  - Department of Microbiology and Immunology, School of Laboratory Medicine and Life 
      Science, Wenzhou Medical University, Wenzhou, China.
AD  - Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, 
      China.
AD  - Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20201008
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7578225
OTO - NOTNLM
OT  - NLRP3
OT  - Vibrio vulnificus
OT  - acidification
OT  - bactericidal activity
OT  - mTOR
OT  - phagolysosome
EDAT- 2020/10/30 06:00
MHDA- 2020/10/30 06:01
PMCR- 2020/01/01
CRDT- 2020/10/29 05:54
PHST- 2020/07/27 00:00 [received]
PHST- 2020/09/07 00:00 [accepted]
PHST- 2020/10/29 05:54 [entrez]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2020/10/30 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2020.587961 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2020 Oct 8;8:587961. doi: 10.3389/fcell.2020.587961. 
      eCollection 2020.