PMID- 33118064
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Linking)
VI  - 48
IP  - 12
DP  - 2020 Dec
TI  - Multi-directional Morphological Assessment of Single Bacterial Colonies Through 
      Non-invasive Optical Imaging.
PG  - 3014-3023
LID - 10.1007/s10439-020-02660-4 [doi]
AB  - A bacterium in bacterial colony is a basal component of bacterial studies and is 
      therefore of considerable importance. The morphological characteristics of a 
      single colony have been widely used as the standard to identify diverse bacterial 
      species. However, the conventional methods for obtaining morphological 
      information, such as microscopic techniques, cannot provide tomographic views. 
      This study utilizes spectral-domain optical coherence tomography (SD-OCT) to 
      observe both external and internal structures for single colonies. OCT imaging 
      provided a clear top (projection) and lateral (cross-sectional) images, which can 
      identify distinct features in the fifteen different bacterial species examined. 
      Through the acquired OCT images, the elevation type in each genus was confirmed, 
      and the quantitative measurements for morphology including a diameter, height, 
      and elevation angle were achieved. Moreover, the OCT raw data was used to build 
      three-dimensional images to observe outer appearances in all direction. As a 
      result, the averaged angles of 7.92 degrees , 14.40 degrees , 6.78 degrees , and 11.92 degrees  of the genus 
      Hymenobacter, Spirosoma, Bacillus, and Deinococcus, respectively, were 
      calculated. In conclusion, we have demonstrated that OCT is a highly feasible, 
      non-invasive method for the identification of bacterial species, providing 
      detailed morphological and visual information. It thus has strong potential for 
      applications in bacteriology, as well as biomedical and food sciences.
FAU - Lee, Junsoo
AU  - Lee J
AD  - School of Electronic and Electrical Engineering, Kyungpook National University, 
      80 Daehak-ro, Buk-gu, Taegu, 41566, South Korea.
FAU - Lee, Seung-Yeol
AU  - Lee SY
AD  - School of Applied Biosciences, Kyungpook National University, 80 Daehak-ro, 
      Buk-gu, Taegu, 41566, South Korea.
FAU - Han, Sangyeob
AU  - Han S
AD  - School of Electronic and Electrical Engineering, Kyungpook National University, 
      80 Daehak-ro, Buk-gu, Taegu, 41566, South Korea.
FAU - Seong, Daewoon
AU  - Seong D
AD  - School of Electronic and Electrical Engineering, Kyungpook National University, 
      80 Daehak-ro, Buk-gu, Taegu, 41566, South Korea.
FAU - Wijesinghe, Ruchire Eranga
AU  - Wijesinghe RE
AD  - Department of Biomedical Engineering, Kyungil University, 50 Gamasil-gil, 
      Hayang-eup, Gyeongsan-si, Gyeongsangbuk-do, 38428, South Korea.
FAU - Kim, Pilun
AU  - Kim P
AD  - School of Medicine, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu, 
      41566, South Korea.
FAU - Jeon, Mansik
AU  - Jeon M
AD  - School of Electronic and Electrical Engineering, Kyungpook National University, 
      80 Daehak-ro, Buk-gu, Taegu, 41566, South Korea.
FAU - Jung, Hee-Young
AU  - Jung HY
AD  - School of Applied Biosciences, Kyungpook National University, 80 Daehak-ro, 
      Buk-gu, Taegu, 41566, South Korea. heeyoung@knu.ac.kr.
FAU - Kim, Jeehyun
AU  - Kim J
AD  - School of Electronic and Electrical Engineering, Kyungpook National University, 
      80 Daehak-ro, Buk-gu, Taegu, 41566, South Korea. jeehk@knu.ac.kr.
LA  - eng
GR  - 2017M3A9E2065282/National Research Foundation of Korea (KR)/
GR  - 2018R1A5A1025137/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20201028
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
SB  - IM
MH  - Bacteria/*cytology
MH  - *Bacteriological Techniques
MH  - *Tomography, Optical Coherence
OTO - NOTNLM
OT  - Bacteriology
OT  - Morphology
OT  - Optical imaging
OT  - Single colony
OT  - Tomographic view
EDAT- 2020/10/30 06:00
MHDA- 2021/09/04 06:00
CRDT- 2020/10/29 05:55
PHST- 2020/08/05 00:00 [received]
PHST- 2020/10/12 00:00 [accepted]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2020/10/29 05:55 [entrez]
AID - 10.1007/s10439-020-02660-4 [pii]
AID - 10.1007/s10439-020-02660-4 [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2020 Dec;48(12):3014-3023. doi: 10.1007/s10439-020-02660-4. Epub 
      2020 Oct 28.