PMID- 33119507
OWN - NLM
STAT- MEDLINE
DCOM- 20210303
LR  - 20210303
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 9
DP  - 2020 Oct 29
TI  - Cellular taxonomy and spatial organization of the murine ventral posterior 
      hypothalamus.
LID - 10.7554/eLife.58901 [doi]
LID - e58901
AB  - The ventral posterior hypothalamus (VPH) is an anatomically complex brain region 
      implicated in arousal, reproduction, energy balance, and memory processing. 
      However, neuronal cell type diversity within the VPH is poorly understood, an 
      impediment to deconstructing the roles of distinct VPH circuits in physiology and 
      behavior. To address this question, we employed a droplet-based single-cell RNA 
      sequencing (scRNA-seq) approach to systematically classify molecularly distinct 
      cell populations in the mouse VPH. Analysis of >16,000 single cells revealed 20 
      neuronal and 18 non-neuronal cell populations, defined by suites of 
      discriminatory markers. We validated differentially expressed genes in selected 
      neuronal populations through fluorescence in situ hybridization (FISH). Focusing 
      on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters 
      that exhibit neuroanatomical parcellation within MB subdivisions and topographic 
      projections to the thalamus. This single-cell transcriptomic atlas of VPH cell 
      types provides a resource for interrogating the circuit-level mechanisms 
      underlying the diverse functions of VPH circuits.
CI  - (c) 2020, Mickelsen et al.
FAU - Mickelsen, Laura E
AU  - Mickelsen LE
AD  - Department of Physiology and Neurobiology, University of Connecticut, Storrs, 
      United States.
AD  - Connecticut Institute for the Brain and Cognitive Sciences, Storrs, United 
      States.
FAU - Flynn, William F
AU  - Flynn WF
AUID- ORCID: 0000-0001-6533-0340
AD  - The Jackson Laboratory for Genomic Medicine, Farmington, United States.
FAU - Springer, Kristen
AU  - Springer K
AD  - Department of Physiology and Neurobiology, University of Connecticut, Storrs, 
      United States.
FAU - Wilson, Lydia
AU  - Wilson L
AD  - Department of Physiology and Neurobiology, University of Connecticut, Storrs, 
      United States.
FAU - Beltrami, Eric J
AU  - Beltrami EJ
AD  - Department of Physiology and Neurobiology, University of Connecticut, Storrs, 
      United States.
FAU - Bolisetty, Mohan
AU  - Bolisetty M
AD  - The Jackson Laboratory for Genomic Medicine, Farmington, United States.
FAU - Robson, Paul
AU  - Robson P
AUID- ORCID: 0000-0002-0191-3958
AD  - The Jackson Laboratory for Genomic Medicine, Farmington, United States.
AD  - Department of Genetics and Genome Sciences, University of Connecticut Health 
      Center, Farmington, United States.
AD  - Institute for Systems Genomics, University of Connecticut, Farmington, United 
      States.
FAU - Jackson, Alexander C
AU  - Jackson AC
AUID- ORCID: 0000-0001-7489-3946
AD  - Department of Physiology and Neurobiology, University of Connecticut, Storrs, 
      United States.
AD  - Connecticut Institute for the Brain and Cognitive Sciences, Storrs, United 
      States.
AD  - Institute for Systems Genomics, University of Connecticut, Farmington, United 
      States.
LA  - eng
SI  - GEO/GSE146692
GR  - R01 MH112739/MH/NIMH NIH HHS/United States
GR  - S10 OD016435/OD/NIH HHS/United States
GR  - 15-RMD-UCHC-01/Connecticut Innovations/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201029
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Profiling
MH  - Hypothalamus, Posterior/anatomy & histology/*cytology/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA/genetics
MH  - Sequence Analysis, RNA
MH  - Single-Cell Analysis
PMC - PMC7595735
OTO - NOTNLM
OT  - cell types
OT  - hypothalamus
OT  - mammillary bodies
OT  - mouse
OT  - neuropeptides
OT  - neuroscience
OT  - neurotransmitters
OT  - single cells
COIS- LM, WF, KS, LW, EB, PR, AJ No competing interests declared, MB Affiliated with 
      Bristol-Myers Squibb. The author has no financial interests to declare.
EDAT- 2020/10/30 06:00
MHDA- 2021/03/04 06:00
PMCR- 2020/10/29
CRDT- 2020/10/29 17:13
PHST- 2020/05/14 00:00 [received]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/10/29 17:13 [entrez]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2021/03/04 06:00 [medline]
PHST- 2020/10/29 00:00 [pmc-release]
AID - 58901 [pii]
AID - 10.7554/eLife.58901 [doi]
PST - epublish
SO  - Elife. 2020 Oct 29;9:e58901. doi: 10.7554/eLife.58901.