PMID- 33120168
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 74
DP  - 2021 Jan
TI  - Effect of maternal nutrient restriction on expression of glucose transporters 
      (SLC2A4 and SLC2A1) and insulin signaling in skeletal muscle of SGA and Non-SGA 
      sheep fetuses.
PG  - 106556
LID - S0739-7240(20)30123-5 [pii]
LID - 10.1016/j.domaniend.2020.106556 [doi]
AB  - Maternal nutrient restriction (NR) causes small for gestational age (SGA) 
      offspring, which are at higher risk for accelerated postnatal growth and 
      developing insulin resistance in adulthood. Skeletal muscle is essential for 
      whole-body glucose metabolism, as 80% of insulin-mediated glucose uptake occurs 
      in this tissue. Maternal NR can alter fetal skeletal muscle mass, expression of 
      glucose transporters, insulin signaling, and myofiber type composition. It also 
      leads to accumulation of intramuscular triglycerides (IMTG), which correlates to 
      insulin resistance. Using a 50% NR treatment from gestational day (GD) 35 to GD 
      135 in sheep, we routinely observe a spectral phenotype of fetal weights within 
      the NR group. Thus, we classified those fetuses into NR(Non-SGA; n = 11) and 
      NR(SGA; n = 11). The control group (n = 12) received 100% of nutrient 
      requirements throughout pregnancy. At GD 135, fetal plasma and gastrocnemius and 
      soleus muscles were collected. In fetal plasma, total insulin was lower in 
      NR(SGA) fetuses compared NR(Non-SGA) and control fetuses (P < 0.01), whereas 
      total IGF-1 was lower in NR(SGA) fetuses compared with control fetuses (P < 
      0.05). Within gastrocnemius, protein expression of insulin receptor (INSRB; P < 
      0.05) and the glucose transporters, solute carrier family 2 member 1 and solute 
      carrier family 2 member 4, was higher (P < 0.05) in NR(SGA) fetuses compared with 
      NR(Non-SGA) fetuses; IGF-1 receptor protein was increased (P < 0.01) in NR(SGA) 
      fetuses compared with control fetuses, and a lower (P < 0.01) proportion of type 
      I myofibers (insulin sensitive and oxidative) was observed in SGA fetuses. For 
      gastrocnemius muscle, the expression of lipoprotein lipase (LPL) messenger RNA 
      (mRNA) was upregulated (P < 0.05) in both NR(SGA) and NR(Non-SGA) fetuses 
      compared with control fetuses, whereas carnitine palmitoyltransferase 1B (CPT1B) 
      mRNA was higher (P < 0.05) in NR(Non-SGA) fetuses compared with control fetuses, 
      but there were no differences (P > 0.05) for protein levels of LPL or CPT1B. 
      Within soleus, there were no differences (P > 0.05) for any characteristic except 
      for the proportion of type I myofibers, which was lower (P < 0.05) in NR(SGA) 
      fetuses compared with control fetuses. Accumulation of IMTG did not differ (P > 
      0.05) in gastrocnemius or soleus muscles. Collectively, the results indicate 
      molecular differences between SGA and Non-SGA fetuses for most characteristics, 
      suggesting that maternal NR induces a spectral phenotype for the metabolic 
      programming of those fetuses.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Sandoval, C
AU  - Sandoval C
AD  - Department of Animal Science, Texas A&M University, College Station, TX 77845, 
      USA; Instituto de Investigaciones Agropecuarias, Region de Magallanes y la 
      Antartica Chilena, Punta Arenas 6212707, Chile.
FAU - Askelson, K
AU  - Askelson K
AD  - Department of Animal Science, Texas A&M University, College Station, TX 77845, 
      USA.
FAU - Lambo, C A
AU  - Lambo CA
AD  - Department of Veterinary Physiology & Pharmacology, Texas A&M University, College 
      Station, TX 77843, USA.
FAU - Dunlap, K A
AU  - Dunlap KA
AD  - Department of Animal Science, Texas A&M University, College Station, TX 77845, 
      USA.
FAU - Satterfield, M C
AU  - Satterfield MC
AD  - Department of Animal Science, Texas A&M University, College Station, TX 77845, 
      USA. Electronic address: csatterfield@tamu.edu.
LA  - eng
GR  - R01 HD080658/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201006
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animal Feed
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Blood Glucose
MH  - Diet/*veterinary
MH  - Female
MH  - Fetal Weight
MH  - Fetus/*drug effects
MH  - Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - Glucose Transporter Type 1/genetics/*metabolism
MH  - Glucose Transporter Type 4/genetics/*metabolism
MH  - Insulin/blood/*metabolism
MH  - Insulin-Like Growth Factor I/genetics/metabolism
MH  - Pregnancy
MH  - Sheep/*embryology
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Insulin signaling
OT  - Maternal nutrient restriction
OT  - Skeletal muscle
OT  - Small for gestational age
OT  - Spectral phenotype
EDAT- 2020/10/30 06:00
MHDA- 2021/12/15 06:00
CRDT- 2020/10/29 20:08
PHST- 2020/03/19 00:00 [received]
PHST- 2020/08/19 00:00 [revised]
PHST- 2020/08/20 00:00 [accepted]
PHST- 2020/10/30 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/10/29 20:08 [entrez]
AID - S0739-7240(20)30123-5 [pii]
AID - 10.1016/j.domaniend.2020.106556 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2021 Jan;74:106556. doi: 10.1016/j.domaniend.2020.106556. 
      Epub 2020 Oct 6.