PMID- 33122828
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20230530
IS  - 1476-5594 (Electronic)
IS  - 0950-9232 (Linking)
VI  - 40
IP  - 2
DP  - 2021 Jan
TI  - Mesenchymal stem cells-derived exosomal microRNA-139-5p restrains tumorigenesis 
      in bladder cancer by targeting PRC1.
PG  - 246-261
LID - 10.1038/s41388-020-01486-7 [doi]
AB  - microRNAs (miRNAs) can be delivered to tumor cells where they exert their 
      function via mesenchymal stem cells (MSCs)-derived exosomes. This study 
      investigated exosomal transfer of miR-139-5p to bladder cancer cells and their 
      role in the regulation of tumorigenesis. The dysregulation of polycomb repressor 
      complex 1 (PRC1) in bladder cancer was characterized by RNA quantification, and 
      its functional significance in bladder cancer cells was identified by 
      loss-of-function experiments. We predicted the miR-139-5p that could play a role 
      in regulating PRC1, which was further verified using dual-luciferase reporter 
      gene assay. Next, we altered the expression of miR-139-5p and PRC1 in bladder 
      cancer cells to identify their functions in cancer progression. Bladder cancer 
      cells were co-cultured with exosomes isolated from human umbilical cord 
      mesenchymal stem cells (hUCMSCs) over-expressing miR-139-5p. The intercellular 
      transfer of miR-139-5p along with in vitro and in vivo functions was determined 
      using gain- and loss-of-function approaches. Our results revealed that PRC1 
      levels were increased in bladder cancer tissues and cells, and silencing PRC1 
      appeared to impede the cell proliferation, migration, and invasion potentials. In 
      addition, miR-139-5p was observed to be down-regulated in bladder cancer, which 
      targeted PRC1 and reduced its expression, hereby resulting in ameliorated 
      tumorigenic characteristics of bladder cancer cells in vitro. Furthermore, we 
      noted that miR-139-5p from hUCMSCs-derived exosomes could be transferred into 
      bladder cancer cells to down-regulate the PRC1 expression. Moreover, 
      hUCMSCs-derived exosomal miR-139-5p conferred a suppressive role on bladder 
      cancer development in vitro and in vivo. These data together supported the 
      tumor-inhibiting role of MSCs-derived exosomal miR-139-5p in bladder cancer, 
      highlighting a promising therapeutic strategy.
FAU - Jia, Yuefeng
AU  - Jia Y
AD  - Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, PR China.
FAU - Ding, Xuemei
AU  - Ding X
AD  - Department of Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, PR China.
FAU - Zhou, Lihua
AU  - Zhou L
AD  - Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, PR China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, PR China.
FAU - Yang, Xuecheng
AU  - Yang X
AUID- ORCID: 0000-0002-5269-2876
AD  - Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, 
      266003, PR China. m18661805062@163.com.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20201029
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (MIRN139 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (PRC1 protein, human)
SB  - IM
CIN - J Urol. 2021 Nov;206(5):1319-1321. PMID: 34392697
RIN - Oncogene. 2023 Jul;42(28):2235. PMID: 37253965
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cell Proliferation
MH  - Exosomes/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/administration & dosage/*genetics
MH  - Middle Aged
MH  - Prognosis
MH  - Survival Rate
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/genetics/metabolism/pathology/*prevention & control
MH  - Xenograft Model Antitumor Assays
EDAT- 2020/10/31 06:00
MHDA- 2021/04/28 06:00
CRDT- 2020/10/30 05:51
PHST- 2020/02/23 00:00 [received]
PHST- 2020/09/21 00:00 [accepted]
PHST- 2020/09/10 00:00 [revised]
PHST- 2020/10/31 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2020/10/30 05:51 [entrez]
AID - 10.1038/s41388-020-01486-7 [pii]
AID - 10.1038/s41388-020-01486-7 [doi]
PST - ppublish
SO  - Oncogene. 2021 Jan;40(2):246-261. doi: 10.1038/s41388-020-01486-7. Epub 2020 Oct 
      29.