PMID- 33122970
OWN - NLM
STAT- MEDLINE
DCOM- 20211006
LR  - 20211006
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2020
DP  - 2020
TI  - Luteolin Attenuates IL-1beta-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression 
      of NF-kappaB and MAPK Pathways.
PG  - 9421340
LID - 10.1155/2020/9421340 [doi]
LID - 9421340
AB  - Cytokine-induced endothelial dysfunction leads to inflammation and vascular 
      adhesion molecule production in retinal pigment epithelium (RPE) cells. 
      Inflammation is a critical mediator in retinal degeneration (RD) diseases, 
      including age-related macular degeneration (AMD), and RD progression may be 
      prevented through anti-inflammatory activity in RPE cells. The flavonoid 
      polyphenol luteolin (LU) has anti-inflammatory and antidiabetes activities, but 
      its effects regarding retinal protection remain unknown. Here, we examined the 
      ability of luteolin to alleviate markers of inflammation related to RD in 
      cytokine-primed APPE-19 cells. We found that luteolin decreased the levels of 
      interleukin- (IL-) 6, IL-8, soluble intercellular adhesion molecule-1 (sICAM-1), 
      and monocyte chemoattractant protein-1 (MCP-1) and attenuated adherence of the 
      human monocytic leukemia cell line THP-1 to IL-1beta-stimulated ARPE-19 cells. 
      Luteolin also increased anti-inflammatory protein heme oxygenase-1 (HO-1) levels. 
      Interestingly, luteolin induced protein kinase B (AKT) phosphorylation, thus 
      inhibiting nuclear factor- (NF-) kappaB transfer from cytoplasm into the nucleus and 
      suppressing mitogen-activated protein kinase (MAPK) inflammatory pathways. 
      Furthermore, cotreatment with MAPK inhibitors and luteolin decreased inflammatory 
      cytokine and chemokine levels, and further suppressed THP-1 adhesion. Overall, 
      these results provide evidence that luteolin protects ARPE-19 cells from 
      IL-1beta-stimulated increases of IL-6, IL-8, sICAM-1, and MCP-1 production by 
      blocking the activation of MAPK and NF-kappaB signaling pathways, thus ameliorating 
      the inflammatory response.
CI  - Copyright (c) 2020 Wen-Chung Huang et al.
FAU - Huang, Wen-Chung
AU  - Huang WC
AUID- ORCID: 0000-0001-7141-8971
AD  - Graduate Institute of Health Industry Technology, Research Center for Food and 
      Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and 
      Technology, Taoyuan City 33303, Taiwan.
AD  - Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital, Linkou, Taoyuan City 33303, Taiwan.
FAU - Liou, Chian-Jiun
AU  - Liou CJ
AD  - Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital, Linkou, Taoyuan City 33303, Taiwan.
AD  - Department of Nursing, Division of Basic Medical Sciences, Research Center for 
      Chinese Herbal Medicine, and Graduate Institute of Health Industry Technology, 
      Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan.
FAU - Shen, Szu-Chuan
AU  - Shen SC
AD  - Graduate Program of Nutrition Science, National Taiwan Normal University, 88 
      Ting-Chow Rd, Sec 4, Taipei, Taiwan.
FAU - Hu, Sindy
AU  - Hu S
AUID- ORCID: 0000-0002-4772-1169
AD  - Department of Cosmetic Science, College of Human Ecology, Chang Gung University 
      of Science and Technology, Guishan Dist., Taoyuan City 33303, Taiwan.
AD  - Department of Dermatology, Aesthetic Medical Center, Chang Gung Memorial 
      Hospital, Linkou, Taoyuan City 33303, Taiwan.
FAU - Hsiao, Chien-Yu
AU  - Hsiao CY
AUID- ORCID: 0000-0003-3301-8808
AD  - Department of Dermatology, Aesthetic Medical Center, Chang Gung Memorial 
      Hospital, Linkou, Taoyuan City 33303, Taiwan.
AD  - Department of Nutrition and Health Sciences, Research Center for Chinese Herbal 
      Medicine, College of Human Ecology, Chang Gung University of Science and 
      Technology, Taoyuan City 33303, Taiwan.
FAU - Wu, Shu-Ju
AU  - Wu SJ
AUID- ORCID: 0000-0003-2370-9541
AD  - Department of Dermatology, Aesthetic Medical Center, Chang Gung Memorial 
      Hospital, Linkou, Taoyuan City 33303, Taiwan.
AD  - Department of Nutrition and Health Sciences, Research Center for Chinese Herbal 
      Medicine, College of Human Ecology, Chang Gung University of Science and 
      Technology, Taoyuan City 33303, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20201016
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - KUX1ZNC9J2 (Luteolin)
SB  - IM
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Interleukin-1beta/*pharmacology
MH  - Luteolin/*pharmacology
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - THP-1 Cells
PMC - PMC7585660
COIS- The authors declare that there is no conflict of interests regarding the 
      publication of this paper.
EDAT- 2020/10/31 06:00
MHDA- 2021/10/07 06:00
PMCR- 2020/10/16
CRDT- 2020/10/30 05:53
PHST- 2020/06/08 00:00 [received]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/08/04 00:00 [accepted]
PHST- 2020/10/30 05:53 [entrez]
PHST- 2020/10/31 06:00 [pubmed]
PHST- 2021/10/07 06:00 [medline]
PHST- 2020/10/16 00:00 [pmc-release]
AID - 10.1155/2020/9421340 [doi]
PST - epublish
SO  - Mediators Inflamm. 2020 Oct 16;2020:9421340. doi: 10.1155/2020/9421340. 
      eCollection 2020.