PMID- 33123595
OWN - NLM
STAT- MEDLINE
DCOM- 20210927
LR  - 20220417
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2020
DP  - 2020
TI  - Circulating T Cells Exhibit Different TIM3/Galectin-9 Expression in Patients with 
      Obesity and Obesity-Related Diabetes.
PG  - 2583257
LID - 10.1155/2020/2583257 [doi]
LID - 2583257
AB  - AIMS: Obesity is highly associated with type 2 diabetes mellitus (T2DM). The 
      TIM3/galectin-9 pathway plays an important role in immune tolerance. Herein, we 
      aimed to investigate the expression of TIM3 and galectin-9 in peripheral blood 
      and to evaluate their clinical significance in patients with obesity and 
      obesity-related T2DM. METHODS: We performed flow cytometry on peripheral blood 
      samples from healthy donors (HC), patients with simple obesity (OB), and patients 
      with obesity comorbid T2DM (OD). The expression of TIM3 on CD3(+), CD4(+), and 
      CD8(+) T cells was determined. The level of galectin-9 in plasma was detected by 
      ELISA. RESULTS: We demonstrated the enhancement of TIM3 on CD3(+), CD4(+), and 
      CD8(+) T cells in the OB group when compared with healthy controls, while it was 
      decreased significantly in the OD group. The TIM3(+)CD8(+) T cells of the OB 
      group were positively correlated with risk factors including BMI, body fat rate, 
      and hipline. The concentration of galectin-9 of the OD group in plasma was 
      significantly higher than that of healthy donors and the OB group. Moreover, the 
      level of galectin-9 of the OD group was positively correlated with fasting 
      insulin and C-peptide, which were two clinical features that represented 
      pancreatic islet function in T2DM. CONCLUSIONS: Our results suggested that TIM3 
      and galectin-9 may be potential biomarkers related to the pathogenesis of 
      obesity-related T2DM.
CI  - Copyright (c) 2020 Lili Sun et al.
FAU - Sun, Lili
AU  - Sun L
AD  - Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical 
      Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, 
      Suzhou, 215006 Jiangsu, China.
AD  - Departments of Endocrinology, The First Affiliated Hospital of Soochow 
      University, Suzhou, 215006 Jiangsu, China.
FAU - Zou, Shengyi
AU  - Zou S
AD  - Departments of Endocrinology, The First Affiliated Hospital of Soochow 
      University, Suzhou, 215006 Jiangsu, China.
FAU - Ding, Sisi
AU  - Ding S
AD  - Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical 
      Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, 
      Suzhou, 215006 Jiangsu, China.
FAU - Du, Xuan
AU  - Du X
AD  - Departments of Endocrinology, The First Affiliated Hospital of Soochow 
      University, Suzhou, 215006 Jiangsu, China.
FAU - Shen, Yu
AU  - Shen Y
AD  - Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical 
      Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, 
      Suzhou, 215006 Jiangsu, China.
FAU - Liu, Cuiping
AU  - Liu C
AUID- ORCID: 0000-0002-7383-1505
AD  - Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical 
      Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, 
      Suzhou, 215006 Jiangsu, China.
FAU - Shi, Bimin
AU  - Shi B
AUID- ORCID: 0000-0002-7032-5367
AD  - Departments of Endocrinology, The First Affiliated Hospital of Soochow 
      University, Suzhou, 215006 Jiangsu, China.
FAU - Zhang, Xueguang
AU  - Zhang X
AD  - Jiangsu Institute of Clinical Immunology & Jiangsu Key Laboratory of Clinical 
      Immunology, First Affiliated Hospital of Soochow University, No. 708 Renmin Road, 
      Suzhou, 215006 Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20201015
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (CD3 Complex)
RN  - 0 (Galectins)
RN  - 0 (HAVCR2 protein, human)
RN  - 0 (Hepatitis A Virus Cellular Receptor 2)
RN  - 0 (LGALS9 protein, human)
SB  - IM
MH  - Adult
MH  - Body Mass Index
MH  - CD3 Complex/biosynthesis
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Diabetes Complications/*blood
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Female
MH  - Flow Cytometry
MH  - Galectins/*blood
MH  - *Gene Expression Regulation
MH  - Hepatitis A Virus Cellular Receptor 2/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Obesity, Morbid/*blood
MH  - T-Lymphocytes/*metabolism
PMC - PMC7585658
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/10/31 06:00
MHDA- 2021/09/28 06:00
PMCR- 2020/10/15
CRDT- 2020/10/30 05:56
PHST- 2020/04/13 00:00 [received]
PHST- 2020/08/24 00:00 [revised]
PHST- 2020/09/09 00:00 [accepted]
PHST- 2020/10/30 05:56 [entrez]
PHST- 2020/10/31 06:00 [pubmed]
PHST- 2021/09/28 06:00 [medline]
PHST- 2020/10/15 00:00 [pmc-release]
AID - 10.1155/2020/2583257 [doi]
PST - epublish
SO  - J Diabetes Res. 2020 Oct 15;2020:2583257. doi: 10.1155/2020/2583257. eCollection 
      2020.