PMID- 33126021
OWN - NLM
STAT- MEDLINE
DCOM- 20201218
LR  - 20201218
IS  - 1618-0941 (Electronic)
IS  - 1434-4610 (Linking)
VI  - 171
IP  - 5
DP  - 2020 Nov
TI  - Biomineralization Capacities of Chlorodendrophyceae: Correlation Between 
      Chloroplast Morphology and the Distribution of Micropearls in the Cell.
PG  - 125760
LID - S1434-4610(20)30065-1 [pii]
LID - 10.1016/j.protis.2020.125760 [doi]
AB  - Several species of the genus Tetraselmis (Chlorodendrophyceae, Chlorophyta) were 
      recently discovered to possess unsuspected biomineralization capacities: they 
      produce multiple intracellular inclusions of amorphous calcium carbonate (ACC), 
      called micropearls. Early light-microscopists had spotted rows of refractive 
      granules in some species, although without identifying their mineral nature. 
      Scanning electron microscope (SEM) observations showed that the distribution of 
      the micropearls in the cell forms a pattern, which appears to be characteristic 
      for a given species. The present study shows that this pattern correlates with 
      the shape of the chloroplast, which differs between Tetraselmis species, because 
      micropearls align themselves along the incisions between chloroplast lobes. This 
      was observed both by SEM and in live cells by light microscopy (LM) using 
      Nomarski differential interference contrast. Additionally, molecular phylogenetic 
      analyses, of rbcL and ITS2 gene sequences from diverse strains of 
      Chlorodendrophyceae, corroborated the morphological observations by identifying 
      two groups among nominal Tetraselmis spp. that differ in chloroplast morphology, 
      micropearl arrangement, and ITS2 RNA secondary structure.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier GmbH.. All rights reserved.
FAU - Martignier, Agathe
AU  - Martignier A
AD  - Department of Earth Sciences, University of Geneva, Geneva 1205, Switzerland. 
      Electronic address: Agathe.Martignier@unige.ch.
FAU - De Respinis, Sophie
AU  - De Respinis S
AD  - Scuola Universitaria Professionale della Svizzera Italiana, Laboratorio 
      microbiologia applicata, Bellinzona 6501, Switzerland.
FAU - Filella, Montserrat
AU  - Filella M
AD  - Department F.-A. Forel, University of Geneva, Geneva 1205, Switzerland.
FAU - Segovia-Campos, Ines
AU  - Segovia-Campos I
AD  - Department of Earth Sciences, University of Geneva, Geneva 1205, Switzerland.
FAU - Marin, Birger
AU  - Marin B
AD  - Institute for Plant Sciences, Department of Biological Sciences, University of 
      Cologne, 50674 Cologne, Germany.
FAU - Gunther, Gerd
AU  - Gunther G
AD  - Knittkuhler Str. 61, 40629 Dusseldorf, Germany.
FAU - Barja, Francois
AU  - Barja F
AD  - MicrobiologyUnit, University of Geneva, Geneva 1211, Switzerland.
FAU - Tonolla, Mauro
AU  - Tonolla M
AD  - MicrobiologyUnit, University of Geneva, Geneva 1211, Switzerland.
FAU - Jaquet, Jean-Michel
AU  - Jaquet JM
AD  - Department of Earth Sciences, University of Geneva, Geneva 1205, Switzerland.
FAU - Melkonian, Michael
AU  - Melkonian M
AD  - Institute for Plant Sciences, Department of Biological Sciences, University of 
      Cologne, 50674 Cologne, Germany; Central Collection of Algal Cultures, Faculty of 
      Biology, University of Duisburg-Essen, 45141 Essen, Germany.
FAU - Ariztegui, Daniel
AU  - Ariztegui D
AD  - Department of Earth Sciences, University of Geneva, Geneva 1205, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200901
PL  - Germany
TA  - Protist
JT  - Protist
JID - 9806488
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Biomineralization
MH  - Calcium Carbonate/*metabolism
MH  - Chlorophyta/*metabolism/*ultrastructure
MH  - Chloroplasts/*ultrastructure
OTO - NOTNLM
OT  - Biomineralization
OT  - Chlorodendrophyceae
OT  - Tetraselmis
OT  - chloroplast morphology.
OT  - intracellular mineral inclusion
OT  - micropearls
EDAT- 2020/10/31 06:00
MHDA- 2020/12/19 06:00
CRDT- 2020/10/30 20:10
PHST- 2020/03/29 00:00 [received]
PHST- 2020/08/17 00:00 [revised]
PHST- 2020/08/22 00:00 [accepted]
PHST- 2020/10/31 06:00 [pubmed]
PHST- 2020/12/19 06:00 [medline]
PHST- 2020/10/30 20:10 [entrez]
AID - S1434-4610(20)30065-1 [pii]
AID - 10.1016/j.protis.2020.125760 [doi]
PST - ppublish
SO  - Protist. 2020 Nov;171(5):125760. doi: 10.1016/j.protis.2020.125760. Epub 2020 Sep 
      1.