PMID- 33126512
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20220910
IS  - 1660-4601 (Electronic)
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Linking)
VI  - 17
IP  - 21
DP  - 2020 Oct 28
TI  - Prenatal Exposure to Gutkha, a Globally Relevant Smokeless Tobacco Product, 
      Induces Hepatic Changes in Adult Mice.
LID - 10.3390/ijerph17217895 [doi]
LID - 7895
AB  - Maternal exposures during pregnancy affect the onset and progression of adult 
      diseases in the offspring. A prior mouse study indicated that maternal tobacco 
      smoke exposure affects hepatic fibrosis in adult offspring. Gutkha, a broadly 
      used smokeless tobacco (ST) product, is widely used by pregnant woman in many 
      countries. The objective of this murine study was to evaluate whether oral 
      maternal exposure to gutkha during pregnancy alters non-alcoholic fatty liver 
      disease (NAFLD) in adult offspring: risk factors for the progression of NAFLD to 
      cirrhosis in adults remain elusive. Buccal cavity 'painting' of pregnant mice 
      with gutkha began on gestational days (GD) 2-4 and continued until parturition. 
      Beginning at 12 weeks of age, a subset of offspring were transitioned to a 
      high-fat diet (HFD). Results demonstrated that prenatal exposure to gutkha 
      followed by an HFD in adulthood significantly increased the histologic evidence 
      of fatty liver disease only in adult male offspring. Changes in hepatic 
      fibrosis-related cytokines (interleukin (IL)-1b and IL-6) and in hepatic collagen 
      mRNA expression were observed when comparing adult male offspring exposed to 
      gutkha in utero to those not exposed. These findings indicate that maternal use 
      of gutkha during pregnancy affects NAFLD in adult offspring in a sex-dependent 
      manner.
FAU - Doherty Lyons, Shannon
AU  - Doherty Lyons S
AUID- ORCID: 0000-0002-0819-5282
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, NY 10010, USA.
FAU - Blum, Jason L
AU  - Blum JL
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, NY 10010, USA.
AD  - Product Safety Labs, Dayton, NJ 08810, USA.
FAU - Hoffman-Budde, Carol
AU  - Hoffman-Budde C
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, NY 10010, USA.
FAU - Tijerina, Pamela B
AU  - Tijerina PB
AUID- ORCID: 0000-0002-4280-3042
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, NY 10010, USA.
FAU - Fiel, M Isabel
AU  - Fiel MI
AD  - Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of 
      Medicine at Mount Sinai, New York, NY 10029, USA.
FAU - J Conklin, Daniel
AU  - J Conklin D
AD  - American Heart Association-Tobacco Regulation and Addiction Center, University of 
      Louisville, Kentucky, KY 40202, USA.
FAU - Gany, Francesca
AU  - Gany F
AD  - Department of Psychiatry and Behavioral Sciences, Memorial Sloan-Kettering Cancer 
      Center, New York, NY 10065, USA.
FAU - Odin, Joseph A
AU  - Odin JA
AD  - Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 
      10029, USA.
FAU - Zelikoff, Judith T
AU  - Zelikoff JT
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, NY 10010, USA.
LA  - eng
GR  - U54HL120163/NH/NIH HHS/United States
GR  - P30 CA008748/CA/NCI NIH HHS/United States
GR  - P30 GM127607/GM/NIGMS NIH HHS/United States
GR  - ES000260/ES/NIEHS NIH HHS/United States
GR  - R01 HL122676/HL/NHLBI NIH HHS/United States
GR  - P20 GM103492/GM/NIGMS NIH HHS/United States
GR  - GM103492/NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201028
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Cytokines)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Collagen
MH  - Cytokines
MH  - Diet, High-Fat
MH  - Female
MH  - Liver/physiopathology
MH  - Male
MH  - Mice
MH  - *Non-alcoholic Fatty Liver Disease/chemically induced
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - *Tobacco, Smokeless/toxicity
PMC - PMC7662769
OTO - NOTNLM
OT  - developmental origins of health and disease
OT  - gutkha
OT  - hepatic
OT  - liver disease
OT  - smokeless tobacco
COIS- The authors declare no conflict of interest.
EDAT- 2020/11/01 06:00
MHDA- 2021/01/23 06:00
PMCR- 2020/11/01
CRDT- 2020/10/31 01:01
PHST- 2020/08/25 00:00 [received]
PHST- 2020/10/07 00:00 [revised]
PHST- 2020/10/15 00:00 [accepted]
PHST- 2020/10/31 01:01 [entrez]
PHST- 2020/11/01 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - ijerph17217895 [pii]
AID - ijerph-17-07895 [pii]
AID - 10.3390/ijerph17217895 [doi]
PST - epublish
SO  - Int J Environ Res Public Health. 2020 Oct 28;17(21):7895. doi: 
      10.3390/ijerph17217895.