PMID- 33128877
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20231112
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 53
IP  - 5
DP  - 2020 Nov 17
TI  - Unbiased Screens Show CD8(+) T Cells of COVID-19 Patients Recognize Shared 
      Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.
PG  - 1095-1107.e3
LID - S1074-7613(20)30447-7 [pii]
LID - 10.1016/j.immuni.2020.10.006 [doi]
AB  - Developing effective strategies to prevent or treat coronavirus disease 2019 
      (COVID-19) requires understanding the natural immune response to severe acute 
      respiratory syndrome coronavirus 2 (SARS-CoV-2). We used an unbiased, genome-wide 
      screening technology to determine the precise peptide sequences in SARS-CoV-2 
      that are recognized by the memory CD8(+) T cells of COVID-19 patients. In total, 
      we identified 3-8 epitopes for each of the 6 most prevalent human leukocyte 
      antigen (HLA) types. These epitopes were broadly shared across patients and 
      located in regions of the virus that are not subject to mutational variation. 
      Notably, only 3 of the 29 shared epitopes were located in the spike protein, 
      whereas most epitopes were located in ORF1ab or the nucleocapsid protein. We also 
      found that CD8(+) T cells generally do not cross-react with epitopes in the four 
      seasonal coronaviruses that cause the common cold. Overall, these findings can 
      inform development of next-generation vaccines that better recapitulate natural 
      CD8(+) T cell immunity to SARS-CoV-2.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Ferretti, Andrew P
AU  - Ferretti AP
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Kula, Tomasz
AU  - Kula T
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Wang, Yifan
AU  - Wang Y
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Nguyen, Dalena M V
AU  - Nguyen DMV
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Weinheimer, Adam
AU  - Weinheimer A
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Dunlap, Garrett S
AU  - Dunlap GS
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Xu, Qikai
AU  - Xu Q
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Nabilsi, Nancy
AU  - Nabilsi N
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Perullo, Candace R
AU  - Perullo CR
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Cristofaro, Alexander W
AU  - Cristofaro AW
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Whitton, Holly J
AU  - Whitton HJ
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Virbasius, Amy
AU  - Virbasius A
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Olivier, Kenneth J Jr
AU  - Olivier KJ Jr
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Buckner, Lyndsey R
AU  - Buckner LR
AD  - Ochsner Medical Center, New Orleans, LA 70121, USA.
FAU - Alistar, Angela T
AU  - Alistar AT
AD  - Atlantic Health System, Morristown, NJ 07960, USA.
FAU - Whitman, Eric D
AU  - Whitman ED
AD  - Atlantic Health System, Morristown, NJ 07960, USA.
FAU - Bertino, Sarah A
AU  - Bertino SA
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - Chattopadhyay, Shrikanta
AU  - Chattopadhyay S
AD  - TScan Therapeutics, Waltham, MA 02451, USA.
FAU - MacBeath, Gavin
AU  - MacBeath G
AD  - TScan Therapeutics, Waltham, MA 02451, USA. Electronic address: 
      gmacbeath@tscan.com.
LA  - eng
PT  - Journal Article
DEP - 20201020
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Coronavirus Nucleocapsid Proteins)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (ORF1ab polyprotein, SARS-CoV-2)
RN  - 0 (Phosphoproteins)
RN  - 0 (Polyproteins)
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (Viral Proteins)
RN  - 0 (nucleocapsid phosphoprotein, SARS-CoV-2)
RN  - 0 (spike protein, SARS-CoV-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Betacoronavirus/*immunology/isolation & purification
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - COVID-19
MH  - Convalescence
MH  - Coronavirus/immunology
MH  - Coronavirus Infections/diagnosis/*immunology
MH  - Coronavirus Nucleocapsid Proteins
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte
MH  - Female
MH  - Humans
MH  - Immunodominant Epitopes
MH  - Immunologic Memory
MH  - Male
MH  - Middle Aged
MH  - Nucleocapsid Proteins/immunology
MH  - Pandemics
MH  - Phosphoproteins
MH  - Pneumonia, Viral/diagnosis/*immunology
MH  - Polyproteins
MH  - SARS-CoV-2
MH  - Spike Glycoprotein, Coronavirus/*immunology
MH  - Viral Proteins/immunology
MH  - Young Adult
PMC - PMC7574860
OTO - NOTNLM
OT  - CD8+ T cells
OT  - COVID-19
OT  - SARS-CoV-2
OT  - T-Scan
OT  - immunity
OT  - immunodominant epitopes
OT  - novel coronavirus
OT  - seasonal coronaviruses
OT  - spike protein
OT  - vaccine
COIS- Declaration of Interests A.P.F., Y.W., D.M.V.N., A.W., G.S.D., Q.X., N.N., 
      C.R.P., A.W.C., H.J.W., A.V., K.J.O., S.A.B., and G.M. are employees of TScan 
      Therapeutics. T.K. is a founder of TScan Therapeutics, and T.K. and G.M. hold 
      equity in TScan Therapeutics. S.C. is a consultant for TScan Therapeutics. A 
      patent application relating to the T-Scan technology used in the current 
      manuscript was filed previously by The Brigham and Women's Hospital, Inc. 
      Provisional patent applications covering SARS-CoV-2 epitope sequences and TCR 
      sequences described in the current manuscript as well as related uses, 
      diagnostics, therapeutics, and vaccines for COVID-19 were filed by TScan 
      Therapeutics, Inc.
EDAT- 2020/11/01 06:00
MHDA- 2020/12/01 06:00
PMCR- 2020/10/20
CRDT- 2020/10/31 20:07
PHST- 2020/07/31 00:00 [received]
PHST- 2020/09/03 00:00 [revised]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2020/11/01 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/10/31 20:07 [entrez]
PHST- 2020/10/20 00:00 [pmc-release]
AID - S1074-7613(20)30447-7 [pii]
AID - 10.1016/j.immuni.2020.10.006 [doi]
PST - ppublish
SO  - Immunity. 2020 Nov 17;53(5):1095-1107.e3. doi: 10.1016/j.immuni.2020.10.006. Epub 
      2020 Oct 20.