PMID- 33129947 OWN - NLM STAT- MEDLINE DCOM- 20210302 LR - 20210302 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 267 DP - 2021 Mar 1 TI - Callicarpa japonica Thunb. ameliorates allergic airway inflammation by suppressing NF-kappaB activation and upregulating HO-1 expression. PG - 113523 LID - S0378-8741(20)33409-7 [pii] LID - 10.1016/j.jep.2020.113523 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Callicarpa japonica Thunb., as an herbal medicine has been used for the treatment of inflammatory diseases in China and Korea. MATERIALS AND METHODS: Ultra performance liquid chromatography-photodiode array-quadrupole time-of-flight mass spectrometer (UPLC-PDA-QTof MS) was used to detect the major phenylethanoid glycosides in the C. japonica extract. BALB/c mice were intraperitoneally sensitized by ovalbumin (OVA) (on days 0 and 7) and challenged by OVA aerosol (on days 11-13) to induce airway inflammatory response. The mice were also administered with C. japonica Thunb. (CJT) (20 and 40 mg/kg Per oral) on days 9-13. CJT pretreatment was conducted in lipopolysaccharide (LPS)-stimulated RAW264.7 or phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells. RESULTS: CJT administration significantly reduced the secretion of Th2 cytokines, TNF-alpha, IL-6, immunoglobulin E (IgE) and histamine, and the recruitment of eosinophils in an OVA-exposed mice. In histological analyses, the amelioration of inflammatory cell influx and mucus secretion were observed with CJT. The OVA-induced airway hyperresponsiveness (AHR), iNOS expression and NF-kappaB activation were effectively suppressed by CJT administration. In addition, CJT led to the upregulation of HO-1 expression. In an in vitro study, CJT pretreatment suppressed the LPS-induced TNF-alpha secretion in RAW264.7 cells and attenuated the PMA-induced IL-6, IL-8 and MCP-1 secretion in A549 cells. These effects were accompanied by downregulated NF-kappaB phosphorylation and by upregulated HO-1 expression. CONCLUSION: These results suggested that CJT has protective activity against OVA-induced airway inflammation via downregulation of NF-kappaB activation and upregulation of HO-1, suggesting that CJT has preventive potential for the development of allergic asthma. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Kim, Seong-Man AU - Kim SM AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea; College of Pharmacy, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Republic of Korea. Electronic address: ksm2906@kribb.re.kr. FAU - Ryu, Hyung Won AU - Ryu HW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea. Electronic address: ryuhw@kribb.re.kr. FAU - Kwon, Ok-Kyoung AU - Kwon OK AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea. Electronic address: dooli@kribb.re.kr. FAU - Hwang, Daseul AU - Hwang D AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea; College of Pharmacy, Chungbuk National University, Cheongju-si, Chungcheongbuk-do, 28160, Republic of Korea. Electronic address: seul3104@kribb.re.kr. FAU - Kim, Min Gu AU - Kim MG AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea; College of Pharmacy, Chungbuk National University, Cheongju-si, Chungcheongbuk-do, 28160, Republic of Korea. Electronic address: alsrn0520@kribb.re.kr. FAU - Min, Jae-Hong AU - Min JH AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea; College of Pharmacy, Chungbuk National University, Cheongju-si, Chungcheongbuk-do, 28160, Republic of Korea. Electronic address: 01020835969@kribb.re.kr. FAU - Zhang, Zhiyun AU - Zhang Z AD - State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, PR China. Electronic address: zhangzy@ibcas.ac.cn. FAU - Kim, Soo-Yong AU - Kim SY AD - International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. Electronic address: soodole@kribb.re.kr. FAU - Paik, Jin-Hyub AU - Paik JH AD - International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. Electronic address: jpaik@kribb.re.kr. FAU - Oh, Sei-Ryang AU - Oh SR AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea. Electronic address: seiryang@kribb.re.kr. FAU - Ahn, Kyung-Seop AU - Ahn KS AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea. Electronic address: ksahn@kribb.re.kr. FAU - Lee, Jae-Won AU - Lee JW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Chungbuk, 28116, Republic of Korea. Electronic address: suc369@kribb.re.kr. LA - eng PT - Journal Article DEP - 20201029 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Anti-Asthmatic Agents) RN - 0 (Anti-Inflammatory Agents) RN - 0 (Cytokines) RN - 0 (Membrane Proteins) RN - 0 (NF-kappa B) RN - 0 (Plant Extracts) RN - 9006-59-1 (Ovalbumin) RN - EC 1.14.14.18 (Heme Oxygenase-1) RN - EC 1.14.14.18 (Hmox1 protein, mouse) SB - IM MH - A549 Cells MH - Animals MH - Anti-Asthmatic Agents/isolation & purification/*pharmacology MH - Anti-Inflammatory Agents/isolation & purification/*pharmacology MH - Asthma/chemically induced/enzymology/physiopathology/*prevention & control MH - Bronchial Hyperreactivity/chemically induced/enzymology/physiopathology/*prevention & control MH - Bronchoconstriction/drug effects MH - *Callicarpa/chemistry MH - Cytokines/metabolism MH - Disease Models, Animal MH - Female MH - Heme Oxygenase-1/*metabolism MH - Humans MH - Lung/*drug effects/enzymology/physiopathology MH - Membrane Proteins/*metabolism MH - Mice MH - Mice, Inbred BALB C MH - NF-kappa B/*metabolism MH - Ovalbumin MH - Plant Extracts/isolation & purification/*pharmacology MH - RAW 264.7 Cells MH - Signal Transduction MH - Up-Regulation OTO - NOTNLM OT - Allergic asthma OT - Callicarpa japonica Thunb. OT - Eosinophil OT - HO-1 OT - NF-kappaB OT - Th2 cytokines EDAT- 2020/11/02 06:00 MHDA- 2021/03/03 06:00 CRDT- 2020/11/01 20:29 PHST- 2020/04/27 00:00 [received] PHST- 2020/10/23 00:00 [revised] PHST- 2020/10/26 00:00 [accepted] PHST- 2020/11/02 06:00 [pubmed] PHST- 2021/03/03 06:00 [medline] PHST- 2020/11/01 20:29 [entrez] AID - S0378-8741(20)33409-7 [pii] AID - 10.1016/j.jep.2020.113523 [doi] PST - ppublish SO - J Ethnopharmacol. 2021 Mar 1;267:113523. doi: 10.1016/j.jep.2020.113523. Epub 2020 Oct 29.