PMID- 33130852
OWN - NLM
STAT- MEDLINE
DCOM- 20210520
LR  - 20231213
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Linking)
VI  - 72
IP  - 4
DP  - 2021 Feb 24
TI  - Isoflavone malonyl-CoA acyltransferase GmMaT2 is involved in nodulation of 
      soybean by modifying synthesis and secretion of isoflavones.
PG  - 1349-1369
LID - 10.1093/jxb/eraa511 [doi]
AB  - Malonyl-CoA:flavonoid acyltransferases (MaTs) modify isoflavones, but only a few 
      have been characterized for activity and assigned to specific physiological 
      processes. Legume roots exude isoflavone malonates into the rhizosphere, where 
      they are hydrolyzed into isoflavone aglycones. Soybean GmMaT2 was highly 
      expressed in seeds, root hairs, and nodules. GmMaT2 and GmMaT4 recombinant 
      enzymes used isoflavone 7-O-glucosides as acceptors and malonyl-CoA as an acyl 
      donor to generate isoflavone glucoside malonates. GmMaT2 had higher activity 
      towards isoflavone glucosides than GmMaT4. Overexpression in hairy roots of 
      GmMaT2 and GmMaT4 produced more malonyldaidzin, malonylgenistin, and 
      malonylglycitin, and resulted in more nodules than control. However, only GmMaT2 
      knockdown (KD) hairy roots showed reduced levels of malonyldaidzin, 
      malonylgenistin, and malonylglycitin, and, likewise, reduced nodule numbers. 
      These were consistent with the up-regulation of only GmMaT2 by rhizobial 
      infection, and higher expression levels of early nodulation genes in GmMaT2- and 
      GmMaT4-overexpressing roots, but lower only in GmMaT2-KD roots compared with 
      control roots. Higher malonyl isoflavonoid levels in transgenic hairy roots were 
      associated with higher levels of isoflavones in root exudates and more nodules, 
      and vice versa. We suggest that GmMaT2 participates in soybean nodulation by 
      catalyzing isoflavone malonylation and affecting malonyl isoflavone secretion for 
      activation of Nod factor and nodulation.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of the 
      Society for Experimental Biology. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Ahmad, Muhammad Zulfiqar
AU  - Ahmad MZ
AD  - State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and 
      Food Science and Technology, Anhui Agricultural University, Hefei, China.
FAU - Zhang, Yanrui
AU  - Zhang Y
AD  - State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and 
      Food Science and Technology, Anhui Agricultural University, Hefei, China.
FAU - Zeng, Xiangsheng
AU  - Zeng X
AD  - College of Agronomy, Anhui Agricultural University, Hefei, China.
FAU - Li, Penghui
AU  - Li P
AD  - State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and 
      Food Science and Technology, Anhui Agricultural University, Hefei, China.
FAU - Wang, Xiaobo
AU  - Wang X
AD  - College of Agronomy, Anhui Agricultural University, Hefei, China.
FAU - Benedito, Vagner A
AU  - Benedito VA
AD  - Division of Plant & Soil Sciences, West Virginia University, Morgantown, WV, USA.
FAU - Zhao, Jian
AU  - Zhao J
AD  - State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and 
      Food Science and Technology, Anhui Agricultural University, Hefei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Isoflavones)
RN  - 524-14-1 (Malonyl Coenzyme A)
RN  - EC 2.3.- (Acyltransferases)
SB  - IM
MH  - Acyltransferases/genetics/*physiology
MH  - *Isoflavones
MH  - Malonyl Coenzyme A/genetics/*physiology
MH  - *Plant Root Nodulation
MH  - *Glycine max/enzymology/genetics
OTO - NOTNLM
OT  - Rhizobium
OT  - Flavonoids
OT  - functional genetics
OT  - legumes
OT  - metabolite modification
OT  - root exudate
OT  - symbiosis
EDAT- 2020/11/02 06:00
MHDA- 2021/05/21 06:00
CRDT- 2020/11/01 20:42
PHST- 2020/07/27 00:00 [received]
PHST- 2020/10/26 00:00 [accepted]
PHST- 2020/11/02 06:00 [pubmed]
PHST- 2021/05/21 06:00 [medline]
PHST- 2020/11/01 20:42 [entrez]
AID - 5948528 [pii]
AID - 10.1093/jxb/eraa511 [doi]
PST - ppublish
SO  - J Exp Bot. 2021 Feb 24;72(4):1349-1369. doi: 10.1093/jxb/eraa511.