PMID- 33130921
OWN - NLM
STAT- MEDLINE
DCOM- 20211109
LR  - 20211109
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Print)
IS  - 0360-3997 (Linking)
VI  - 44
IP  - 2
DP  - 2021 Apr
TI  - Motility and Mechanical Properties of Dendritic Cells Deteriorated by 
      Extracellular Acidosis.
PG  - 737-745
LID - 10.1007/s10753-020-01373-z [doi]
AB  - Dendritic cells (DCs) are the most powerful antigen-presenting cells known to 
      date and play an important role in initiating and amplifying both innate and 
      adaptive immune responses. Extracellular acidosis is an important hallmark of a 
      variety of inflammatory processes and solid tumors. However, few studies have 
      focused on the effect of extracellular acidosis on DCs and their functions. 
      Cellular mechanical properties reflect the relationship between cell structure 
      and function, including cytoskeleton (especially F-actin organization), membrane 
      negative charges, membrane fluidity, and osmotic fragility. The study 
      investigated the effects of extracellular acidosis on the DCs functions from the 
      perspective of cellular migration and mechanical properties. The results showed 
      that migration ability, F-actin contents, and membrane negative charges of DCs 
      were reduced by extracellular acidosis no matter whether LPS stimulated its 
      maturation or not. And these functions could not return to normal after removing 
      acidic microenvironment, which revealed that the function impairment induced by 
      extracellular acidosis might be irreversible. In addition, the proliferation 
      capacity of stimulated allogeneic T cells was impaired by extracellular acidosis. 
      Our results suggest extracellular acidosis may play an immunosuppressive role in 
      DCs-mediated immune process.
FAU - Tong, Lu
AU  - Tong L
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China.
FAU - Yue, Ping
AU  - Yue P
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China.
FAU - Yang, Yingying
AU  - Yang Y
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China.
FAU - Huang, Jin
AU  - Huang J
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China.
FAU - Zeng, Zhu
AU  - Zeng Z
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China. zengzhu100@sina.com.
FAU - Qiu, Wei
AU  - Qiu W
AUID- ORCID: 0000-0001-8823-5733
AD  - School of Biology and Engineering, Guizhou Medical University, Guiyang, 550025, 
      China. qwei407@163.com.
LA  - eng
GR  - 31960206/National Natural Science Foundation of China (CN)/
GR  - gzwjkj2019-1-078/Department of Health of Guizhou Province/
GR  - J-[2020]1Y334/Guizhou Science and Technology Department/
PT  - Journal Article
DEP - 20201031
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
SB  - IM
MH  - Acidosis/*immunology/physiopathology
MH  - Animals
MH  - Cell Movement/*immunology
MH  - Cell Proliferation
MH  - Cell Survival/immunology
MH  - Cells, Cultured
MH  - Cellular Microenvironment/*immunology
MH  - Cytoskeleton/*immunology
MH  - Dendritic Cells/*immunology/physiology
MH  - Membrane Fluidity/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Osmotic Fragility/*immunology
PMC - PMC7985054
OTO - NOTNLM
OT  - cellular mechanical properties
OT  - dendritic cells
OT  - extracellular acidosis
OT  - motility
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/11/02 06:00
MHDA- 2021/11/10 06:00
PMCR- 2020/10/31
CRDT- 2020/11/01 20:42
PHST- 2020/03/30 00:00 [received]
PHST- 2020/10/21 00:00 [accepted]
PHST- 2020/11/02 06:00 [pubmed]
PHST- 2021/11/10 06:00 [medline]
PHST- 2020/11/01 20:42 [entrez]
PHST- 2020/10/31 00:00 [pmc-release]
AID - 10.1007/s10753-020-01373-z [pii]
AID - 1373 [pii]
AID - 10.1007/s10753-020-01373-z [doi]
PST - ppublish
SO  - Inflammation. 2021 Apr;44(2):737-745. doi: 10.1007/s10753-020-01373-z. Epub 2020 
      Oct 31.