PMID- 33131385
OWN - NLM
STAT- MEDLINE
DCOM- 20211001
LR  - 20211001
IS  - 1557-9891 (Electronic)
IS  - 1557-9883 (Print)
IS  - 1557-9883 (Linking)
VI  - 14
IP  - 5
DP  - 2020 Sep-Oct
TI  - Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of 
      Interleukin-6 and Affect Sperm Quality.
PG  - 1557988320970053
LID - 10.1177/1557988320970053 [doi]
LID - 1557988320970053
AB  - Reproductive tract inflammation is considered an important cause of male 
      infertility. Increased leukocytes in semen can produce many reactive oxygen 
      species (ROS), which affect sperm function. The aim of this study is to identify 
      the main source of ROS in seminal plasma and to assess the effect of ROS on 
      leukocytes. Semen samples (n = 20) with leukocyte concentration >1 x 10(6) were 
      collected from a male infertility clinic. This study mainly compares the sperm 
      function parameters of the normal group and the semen white blood cell group >1 x 
      10(6). The results identified that ROS in semen was closely related to sperm 
      function parameters, and CD45(+) leucocytes were the main source of ROS. Compared 
      with the control group, the concentration of IL-2, IL-4, IL-6, IFN-gamma, and TNF-alpha 
      was higher in the experimental group. Leukocytes in semen may regulate the 
      secretion of ROS through the mammalian target of rapamycin (mTOR) pathway. A 
      considerable amount of ROS can upregulate the expression of IL-6 in leukocytes 
      via the nuclear factor kappa-B (NF-kB) pathway.
FAU - Li, Xiaoping
AU  - Li X
AD  - Center for Reproduction and Genetics, The First Affiliated Hospital of Soochow 
      University, SuZhou, Jiangsu, China.
AD  - Biomedical Research Institute, Soochow University, SuZhou, Jiangsu, China.
FAU - Ni, Mengxia
AU  - Ni M
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Xing, Shiyu
AU  - Xing S
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Yu, Yi
AU  - Yu Y
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Zhou, Yan
AU  - Zhou Y
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Yang, Shenmin
AU  - Yang S
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Li, Hong
AU  - Li H
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Zhu, Rui
AU  - Zhu R
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
FAU - Han, Mutian
AU  - Han M
AUID- ORCID: 0000-0002-2456-4893
AD  - Department of Immunology, Anhui Medical University, HeFei, China.
AD  - Center for Reproduction and Genetics, Nanjing Medical University Affiliated 
      Suzhou Hospital, SuZhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Mens Health
JT  - American journal of men's health
JID - 101287723
RN  - 0 (Interleukin-6)
RN  - 0 (Reactive Oxygen Species)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Humans
MH  - Infertility, Male/*metabolism
MH  - Interleukin-6
MH  - Leukocytes
MH  - Male
MH  - Oxygen/*metabolism
MH  - *Reactive Oxygen Species
MH  - Semen/*metabolism
MH  - Spermatozoa/*metabolism
PMC - PMC7607761
OTO - NOTNLM
OT  - IL-6
OT  - Leukocytes
OT  - NF-kB
OT  - mTOR
OT  - reactive oxygen species
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/11/03 06:00
MHDA- 2021/10/02 06:00
PMCR- 2020/10/31
CRDT- 2020/11/02 05:31
PHST- 2020/11/02 05:31 [entrez]
PHST- 2020/11/03 06:00 [pubmed]
PHST- 2021/10/02 06:00 [medline]
PHST- 2020/10/31 00:00 [pmc-release]
AID - 10.1177_1557988320970053 [pii]
AID - 10.1177/1557988320970053 [doi]
PST - ppublish
SO  - Am J Mens Health. 2020 Sep-Oct;14(5):1557988320970053. doi: 
      10.1177/1557988320970053.