PMID- 33132239
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20211227
IS  - 1349-8037 (Electronic)
IS  - 0022-1260 (Linking)
VI  - 67
IP  - 4
DP  - 2021 Oct 22
TI  - Corynebacterium glutamicum Mycoredoxin 3 protects against multiple oxidative 
      stresses and displays thioredoxin-like activity.
PG  - 125-133
LID - 10.2323/jgam.2019.10.003 [doi]
AB  - Glutaredoxins (Grxs) and thioredoxins (Trxs) play a critical role in resistance 
      to oxidative conditions. However, physiological and biochemical roles of 
      Mycoredoxin 3 (Mrx3) that shared a high amino acid sequence similarity to Grxs 
      remain unknown in Corynebacterium glutamicum. Here we showed that mrx3 deletion 
      strains of C. glutamicum was involved in the protection against oxidative stress. 
      Recombinant Mrx3 not only catalytically reduced the disulfide bonds in 
      ribonucleotide reductase (RNR), insulin and 5,5'-dithiobis-(2-nitro-benzoicacid) 
      (DTNB), but also reduced the mixed disulphides between mycothiol (MSH) and 
      substrate, which was exclusively linked to the thioredoxin reductase (TrxR) 
      electron transfer pathway by a dithiol mechanism. Site-directed mutagenesis 
      confirmed that the conserved Cys17 and Cys20 in Mrx3 were necessary to maintain 
      its activity. The mrx3 deletion mutant showed decreased resistance to various 
      stress, and these sensitive phenotypes were almost fully restored in the 
      complementary strain. The physiological roles of Mrx3 in resistance to various 
      stress were further supported by the induced expression of mrx3 under various 
      stress conditions, directly under the control of the stress-responsive 
      extracytoplasmic function-sigma (ECF-sigma) factor SigH. Thus, we presented the first 
      evidence that Mrx3 protected against various oxidative stresses by acting as a 
      disulfide oxidoreductase behaving like Trx.
FAU - Su, Tao
AU  - Su T
AD  - College of Life Sciences, Qufu Normal University.
FAU - Che, Chengchuan
AU  - Che C
AD  - College of Life Sciences, Qufu Normal University.
FAU - Sun, Ping
AU  - Sun P
AD  - College of Life Sciences, Qufu Normal University.
FAU - Li, Xiaona
AU  - Li X
AD  - College of Life Sciences, Qufu Normal University.
FAU - Gong, Zhijin
AU  - Gong Z
AD  - College of Life Sciences, Qufu Normal University.
FAU - Liu, Jinfeng
AU  - Liu J
AD  - College of Life Sciences, Qufu Normal University.
FAU - Yang, Ge
AU  - Yang G
AD  - College of Life Sciences, Qufu Normal University.
LA  - eng
PT  - Journal Article
DEP - 20201030
PL  - Japan
TA  - J Gen Appl Microbiol
JT  - The Journal of general and applied microbiology
JID - 0165543
RN  - 0 (Bacterial Proteins)
RN  - 0 (Glutaredoxins)
SB  - IM
MH  - Bacterial Proteins/chemistry/*genetics/metabolism
MH  - Corynebacterium glutamicum/chemistry/*genetics/metabolism
MH  - Gene Deletion
MH  - Genes, Bacterial
MH  - Glutaredoxins/chemistry/*genetics/metabolism
MH  - Stress, Physiological
OTO - NOTNLM
OT  - Mycoredoxin 3
OT  - enzyme activity
OT  - glutaredoxin-like proteins
OT  - redox active disulfide
OT  - redox regulation
EDAT- 2020/11/03 06:00
MHDA- 2021/12/28 06:00
CRDT- 2020/11/02 06:11
PHST- 2020/11/03 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2020/11/02 06:11 [entrez]
AID - 10.2323/jgam.2019.10.003 [doi]
PST - ppublish
SO  - J Gen Appl Microbiol. 2021 Oct 22;67(4):125-133. doi: 10.2323/jgam.2019.10.003. 
      Epub 2020 Oct 30.