PMID- 33133252
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201103
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 20
IP  - 6
DP  - 2020 Dec
TI  - ROS1 rearrangements are uncommon in biliary tract cancers.
PG  - 316
LID - 10.3892/ol.2020.12179 [doi]
LID - 316
AB  - Biliary tract cancers (BTCs) are a pool of diseases with poor prognosis and there 
      is no orphan drug available. Currently, no molecular targets have been tested as 
      druggable oncogenic drivers. C-ros oncogene 1 (ROS1) rearrangements have been 
      previously described in various tumors, including BTCs; however, data regarding 
      their incidence and biological significance are controversial. Therefore, a 
      retrospective multicenter study was performed to assess the incidence of ROS1 
      rearrangements in BTCs by means of immunohistochemistry and fluorescence in situ 
      hybridization (FISH). The present study failed to demonstrate ROS1 expression in 
      a multicenter series of 150 cases with BTCs and revealed that D4D6 was the most 
      specific clone compared with other ROS1 primary antibodies, namely PA1-30318 and 
      EPMGHR2. Notably, negative results obtained with D4D6 completely matched to data 
      sorted out by FISH analysis, thus confirming a lack of ROS1 gene rearrangements 
      in BTCs and false positive results when PA1-30318 and EPMGHR2 clones were used. 
      These results suggest that ROS1 rearrangements may not be targets for molecular 
      therapy of BTCs with specific inhibitors.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Mazzoni, Francesca
AU  - Mazzoni F
AD  - Medical Oncology Unit, AOU Careggi Hospital, I-50134 Florence, Italy.
FAU - Petreni, Paolo
AU  - Petreni P
AD  - Medical Oncology Unit, Alta Val d'Elsa Hospital, I-53036 Poggibonsi, Italy.
FAU - Vasile, Enrico
AU  - Vasile E
AD  - Medical Oncology Unit, AOU Pisana Hospital, I-56126 Pisa, Italy.
FAU - Panebianco, Michele
AU  - Panebianco M
AD  - AUSL-IRCSS, I-42122 Reggio Emilia, Italy.
FAU - Casadei-Gardini, Andrea
AU  - Casadei-Gardini A
AD  - Department of Medical Oncology, IRST IRCCS, I-47014 Meldola, Italy.
FAU - Negri, Francesca
AU  - Negri F
AD  - Medical Oncology Unit, AOU Parma Hospital, I-43126 Parma, Italy.
FAU - Lunghi, Alice
AU  - Lunghi A
AD  - Medical Oncology Unit, San Luca Hospital, I-55100 Lucca, Italy.
FAU - Pillozzi, Serena
AU  - Pillozzi S
AD  - Medical Oncology Unit, AOU Careggi Hospital, I-50134 Florence, Italy.
AD  - Department of Experimental and Clinical Medicine, University of Florence, I-50134 
      Florence, Italy.
FAU - Vivaldi, Caterina
AU  - Vivaldi C
AD  - Medical Oncology Unit, AOU Pisana Hospital, I-56126 Pisa, Italy.
FAU - Gervasi, Erika
AU  - Gervasi E
AD  - AUSL-IRCSS, I-42122 Reggio Emilia, Italy.
FAU - Frassineti, Giovanni Luca
AU  - Frassineti GL
AD  - Department of Medical Oncology, IRST IRCCS, I-47014 Meldola, Italy.
FAU - Messerini, Luca
AU  - Messerini L
AD  - Anatomical Pathology Unit, AOU Careggi Hospital, I-50134 Florence, Italy.
FAU - Jocolle, Genny
AU  - Jocolle G
AD  - Regional Parini Hospital, I-11100 Aosta, Italy.
FAU - Bisagni, Alessandra
AU  - Bisagni A
AD  - Pathology Unit, ASL-IRCCS, I-42122 Reggio Emilia, Italy.
FAU - Antonuzzo, Lorenzo
AU  - Antonuzzo L
AD  - Medical Oncology Unit, AOU Careggi Hospital, I-50134 Florence, Italy.
FAU - Rossi, Giulio
AU  - Rossi G
AD  - Operative Unit of Pathologic Anatomy, Santa Maria delle Croci Hospital, I-48121 
      Ravenna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20201001
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC7590438
OTO - NOTNLM
OT  - ROS1 rearrangements
OT  - biliary tract cancers
OT  - biomarker
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - molecular target
EDAT- 2020/11/03 06:00
MHDA- 2020/11/03 06:01
PMCR- 2020/10/01
CRDT- 2020/11/02 06:16
PHST- 2019/08/14 00:00 [received]
PHST- 2020/03/31 00:00 [accepted]
PHST- 2020/11/02 06:16 [entrez]
PHST- 2020/11/03 06:00 [pubmed]
PHST- 2020/11/03 06:01 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - OL-0-0-12179 [pii]
AID - 10.3892/ol.2020.12179 [doi]
PST - ppublish
SO  - Oncol Lett. 2020 Dec;20(6):316. doi: 10.3892/ol.2020.12179. Epub 2020 Oct 1.