PMID- 33134255
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201103
IS  - 2296-2646 (Print)
IS  - 2296-2646 (Electronic)
IS  - 2296-2646 (Linking)
VI  - 8
DP  - 2020
TI  - Functional Up-Conversion Nanoparticle-Based Immunochromatography Assay for 
      Simultaneous and Sensitive Detection of Residues of Four Tetracycline Antibiotics 
      in Milk.
PG  - 759
LID - 10.3389/fchem.2020.00759 [doi]
LID - 759
AB  - An ultrahigh-sensitivity lateral flow immunochromatography (LFIC) assay based on 
      up-converting nanoparticles (UCNPs) was developed to carry out a multi-residue 
      detection of tetracycline in milk. The sensitivity of the immunoassay was greatly 
      improved by the use of a broad-spectrum monoclonal antibody attached to UCNPs to 
      form a signal probe. Under the optimal conditions, the UCNP-LFIC assay enabled 
      sensitive detection of tetracycline (TC) as well as of oxytetracycline (OTC), 
      chlortetracycline (CTC), and doxycycline (DOX) within 10 min, with IC (50) values 
      of 0.32, 0.32, 0.26, 0.22 ng/mL, respectively. There was no cross-reactivity with 
      ten other antibiotics. Similarly, we evaluated the experimental results for 
      matrix effects. Experiments involving spiking showed the four tetracycline 
      antibiotics displaying mean recoveries ranging from 93.95 to 111.90% with 
      relative standard deviations (RSDs) of < 9.95%. The detection results of actual 
      samples using the developed method showed a good correlation (R (2) >/= 0.98) with 
      the results using high-performance liquid chromatography (HPLC). Thus, the assay 
      can achieve an ultrahighly sensitive detection of antibiotics in milk, and can 
      hence promote human health and provides promising applications in the 
      bio-detection field.
CI  - Copyright (c) 2020 Xu, Ma, Chen, Yu, Sun and Zhang.
FAU - Xu, Ying
AU  - Xu Y
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, China.
FAU - Ma, Biao
AU  - Ma B
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, China.
FAU - Chen, Erjing
AU  - Chen E
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, China.
FAU - Yu, Xiaoping
AU  - Yu X
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, China.
FAU - Sun, Chuanxin
AU  - Sun C
AD  - Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, 
      Swedish University of Agricultural Science (SLU), Uppsala, Sweden.
FAU - Zhang, Mingzhou
AU  - Zhang M
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20201008
PL  - Switzerland
TA  - Front Chem
JT  - Frontiers in chemistry
JID - 101627988
PMC - PMC7578426
OTO - NOTNLM
OT  - immunoassay
OT  - milk
OT  - multi-residue detection
OT  - tetracycline
OT  - up-conversion nanoparticle
EDAT- 2020/11/03 06:00
MHDA- 2020/11/03 06:01
PMCR- 2020/01/01
CRDT- 2020/11/02 06:20
PHST- 2020/04/28 00:00 [received]
PHST- 2020/07/22 00:00 [accepted]
PHST- 2020/11/02 06:20 [entrez]
PHST- 2020/11/03 06:00 [pubmed]
PHST- 2020/11/03 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fchem.2020.00759 [doi]
PST - epublish
SO  - Front Chem. 2020 Oct 8;8:759. doi: 10.3389/fchem.2020.00759. eCollection 2020.