PMID- 33137394
OWN - NLM
STAT- MEDLINE
DCOM- 20220124
LR  - 20220124
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 116
DP  - 2021 Mar
TI  - CqPP2A inhibits white spot syndrome virus infection by up-regulating 
      antimicrobial substances expression in red claw crayfish Cherax quadricarinatus.
PG  - 103913
LID - S0145-305X(20)30468-7 [pii]
LID - 10.1016/j.dci.2020.103913 [doi]
AB  - Protein phosphatase 2A (PP2A) is an important serine/threonine phosphatase, a 
      highly conserved enzyme widely expressed in eukaryotic cells, which accounts for 
      a majority of the serine/threonine phosphatase activity in cells implicated in 
      regulation of immune signaling pathways and antiviral response. However, most of 
      studies about PP2A have been conducted in mammals but few in crustaceans. In this 
      study, two subunits of PP2A (named as CqPP2Ab and CqPP2Ac) were characterized to 
      be involved in white spot syndrome virus (WSSV) infection in the haematopoietic 
      tissue (Hpt) cells from red claw crayfish Cherax quadricarinatus. The open 
      reading frame (ORF) of CqPP2Ab was 1341 bp encoding 446 amino acids with seven 
      WD40 domains, and the ORF of CqPP2Ac was 930 bp encoding 309 amino acids with a 
      PP2Ac domain. Tissue distribution analysis showed that the mRNA transcript of 
      CqPP2Ab and CqPP2Ac were both widely expressed in all the tested tissues with the 
      highest expression in hemocyte, followed by high expression in Hpt. The gene 
      expressions of CqPP2Ab and CqPP2Ac were both significantly down-regulated at 6 h 
      post WSSV infection (6 hpi) in Hpt cells. Importantly, the expression of viral 
      immediate early gene IE1 and late viral gene envelope protein VP28 were both 
      significantly increased post WSSV infection after gene silencing of CqPP2Ab or 
      CqPP2Ac in Hpt cells, suggesting that CqPP2Ab and CqPP2Ac could inhibit WSSV 
      infection in Hpt cells, probably by increasing the antimicrobial substances 
      expression in consideration to the significantly reduced expression of 
      anti-lipopolysaccharide factor, crustin, and lysozyme after gene silencing of 
      CqPP2Ab or CqPP2Ac, respectively. These findings provide a new light on the 
      mechanism of WSSV infection and the antiviral response in crustaceans.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Gao, Rui-Lin
AU  - Gao RL
AD  - State Key Laboratory of Marine Environmental Science, State-Province Joint 
      Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and 
      Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
FAU - Liu, Ling-Ke
AU  - Liu LK
AD  - State Key Laboratory of Marine Environmental Science, State-Province Joint 
      Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and 
      Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
FAU - Guo, Li-Mei
AU  - Guo LM
AD  - State Key Laboratory of Marine Environmental Science, State-Province Joint 
      Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and 
      Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
FAU - Wang, Ke-Jian
AU  - Wang KJ
AD  - State Key Laboratory of Marine Environmental Science, State-Province Joint 
      Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and 
      Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
FAU - Liu, Hai-Peng
AU  - Liu HP
AD  - State Key Laboratory of Marine Environmental Science, State-Province Joint 
      Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and 
      Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China; Laboratory for 
      Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science 
      and Technology, Qingdao, China. Electronic address: Haipengliu@xmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201101
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antimicrobial Peptides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Protein Subunits)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antimicrobial Peptides/genetics/*immunology/metabolism
MH  - Arthropod Proteins/genetics/*immunology/metabolism
MH  - Astacoidea/genetics/*immunology/virology
MH  - Base Sequence
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation/*immunology
MH  - Hematopoietic System/cytology/immunology/metabolism
MH  - Hemocytes/cytology/immunology/metabolism
MH  - Host-Pathogen Interactions/immunology
MH  - Immunity, Innate/genetics/immunology
MH  - Protein Phosphatase 2/genetics/*immunology/metabolism
MH  - Protein Subunits/genetics/immunology/metabolism
MH  - Sequence Analysis, DNA/methods
MH  - Sequence Homology, Amino Acid
MH  - White spot syndrome virus 1/*immunology/physiology
OTO - NOTNLM
OT  - Antimicrobial substances
OT  - Antiviral immunity
OT  - Cherax quadricarinatus
OT  - Crustacean
OT  - Protein phosphatase 2A
OT  - White spot syndrome virus
EDAT- 2020/11/03 06:00
MHDA- 2022/01/27 06:00
CRDT- 2020/11/02 20:11
PHST- 2020/09/23 00:00 [received]
PHST- 2020/10/27 00:00 [revised]
PHST- 2020/10/27 00:00 [accepted]
PHST- 2020/11/03 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2020/11/02 20:11 [entrez]
AID - S0145-305X(20)30468-7 [pii]
AID - 10.1016/j.dci.2020.103913 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2021 Mar;116:103913. doi: 10.1016/j.dci.2020.103913. Epub 2020 
      Nov 1.