PMID- 33140607
OWN - NLM
STAT- MEDLINE
DCOM- 20210115
LR  - 20230809
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 37
IP  - 5
DP  - 2020 Oct 25
TI  - [Study on visfatin-induced inflammation and necroptosis via LOX-1 in human 
      umbilical vein endothelial cells].
PG  - 834-841
LID - 10.7507/1001-5515.202003067 [doi]
AB  - The aim of the study is to identify the effects and underlying mechanisms of 
      visfatin on inflammation and necroptosis in vascular endothelial cells. Human 
      umbilical vein endothelial cells (HUVECs) were stimulated with visfatin or 
      pretreated with Polyinosinic acid (LOX-1 inhibitor). By using the Western blot, 
      RT-PCR, immunocytochemistry, enzyme-linked immunosorbent assay (ELISA), MTT and 
      flow cytometry technique, the occurrence of inflammation and necroptosis in 
      HUVECs were evaluated. Our results showed that 100 ng/mL visfatin significantly 
      increased the mRNA and protein expression of monocyte chemotactic protein 1 
      (MCP-1) and LOX-1 after 24 hours' treatment in HUVECs. However, pretreatment with 
      Polyinosinic acid could significantly reduce the expression of MCP-1 compared 
      with visfatin group. Additionally, 100 ng/mL visfatin could induce the production 
      of necrotic features and increase the mRNA expression of BMF (one of the markers 
      of necroptosis), while pretreating with Polyinosinic acid markedly downregulated 
      the mRNA expression of BMF gene and promoted the cell proliferation. These 
      results indicate that visfatin might induce inflammation and necroptosis via 
      LOX-1 in HUVECs, suggesting that visfatin plays a central role in the development 
      of atherosclerosis.
FAU - Han, Xiaoyu
AU  - Han X
AD  - Geriatrics Department, Chengdu Second People's Hospital, Chengdu 610017, 
      P.R.China;Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan 
      University, Chengdu 610041, P.R.China.
FAU - Wu, Wenchao
AU  - Wu W
AD  - Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan University, 
      Chengdu 610041, P.R.China.
FAU - Liu, Xiaojing
AU  - Liu X
AD  - Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan University, 
      Chengdu 610041, P.R.China;Vasculocardiology Department, West China Hospital, 
      Sichuan University, Chengdu 610041, P.R.China.
FAU - Zhu, Ye
AU  - Zhu Y
AD  - Vasculocardiology Department, West China Hospital, Sichuan University, Chengdu 
      610041, P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (OLR1 protein, human)
RN  - 0 (Scavenger Receptors, Class E)
RN  - EC 2.4.2.12 (Nicotinamide Phosphoribosyltransferase)
SB  - IM
MH  - Cells, Cultured
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Inflammation/chemically induced
MH  - *Necroptosis
MH  - Nicotinamide Phosphoribosyltransferase
MH  - Scavenger Receptors, Class E/genetics
PMC - PMC10320536
OTO - NOTNLM
OT  - LOX-1
OT  - human umbilical vein endothelial cells
OT  - inflammation
OT  - necroptosis
OT  - visfatin
COIS- 利益冲突声明：本文全体作者均声明不存在利益冲突。
EDAT- 2020/11/04 06:00
MHDA- 2021/01/16 06:00
PMCR- 2020/10/25
CRDT- 2020/11/03 05:52
PHST- 2020/11/03 05:52 [entrez]
PHST- 2020/11/04 06:00 [pubmed]
PHST- 2021/01/16 06:00 [medline]
PHST- 2020/10/25 00:00 [pmc-release]
AID - swyxgcxzz-37-5-834 [pii]
AID - 10.7507/1001-5515.202003067 [doi]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2020 Oct 25;37(5):834-841. doi: 
      10.7507/1001-5515.202003067.