PMID- 33142116
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 267
DP  - 2021 Jan
TI  - Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale 
      favors alpha(5)beta(1) integrin-mediated adhesion.
PG  - 120484
LID - S0142-9612(20)30730-4 [pii]
LID - 10.1016/j.biomaterials.2020.120484 [doi]
AB  - Here we present the use of surface nanopatterning of covalently immobilized BMP-2 
      and integrin selective ligands to determine the specificity of their interactions 
      in regulating cell adhesion and focal adhesion assembly. Gold nanoparticle arrays 
      carrying single BMP-2 dimers are prepared by block-copolymer micellar 
      nanolithography and azide-functionalized integrin ligands (cyclic-RGD peptides or 
      alpha(5)beta(1) integrin peptidomimetics) are immobilized on the surrounding 
      polyethylene glycol alkyne by click chemistry. Compared to BMP-2 added to the 
      media, surface immobilized BMP-2 (iBMP-2) favors the spatial segregation of 
      adhesion clusters and enhances focal adhesion (FA) size in cells adhering to 
      alpha(5)beta(1) integrin selective ligands. Moreover, iBMP-2 copresented with alpha(5)beta(1) 
      integrin ligands induces the recruitment of alpha(v)beta(3) integrins in FAs. When 
      copresented with RGD, iBMP-2 induces the assembly of a higher number of FAs, 
      which are not affected by alpha(5)beta(1) integrin blocking. Our dual-functionalized 
      platforms offer the possibility to study the crosstalk between integrins and BMP 
      receptors, and more in general they could be used to address the spatial 
      regulation of growth factors and adhesion receptors crosstalk on biomimetic 
      surfaces.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Posa, Francesca
AU  - Posa F
AD  - Max Planck Institute for Medical Research, Department of Cellular Biophysics, 
      Jahnstrasse 29, Heidelberg, 69120, Germany.
FAU - Baha-Schwab, Elisabeth H
AU  - Baha-Schwab EH
AD  - Max Planck Institute for Medical Research, Department of Cellular Biophysics, 
      Jahnstrasse 29, Heidelberg, 69120, Germany.
FAU - Wei, Qiang
AU  - Wei Q
AD  - Max Planck Institute for Medical Research, Department of Cellular Biophysics, 
      Jahnstrasse 29, Heidelberg, 69120, Germany.
FAU - Di Benedetto, Adriana
AU  - Di Benedetto A
AD  - University of Foggia, Department of Clinical and Experimental Medicine, viale 
      Pinto 1, Foggia, 71122, Italy.
FAU - Neubauer, Stefanie
AU  - Neubauer S
AD  - Institute for Advanced Study and Center of Integrated Protein Science (CIPSM), 
      Department of Chemistry, Technische Universitat Munchen, Lichtenbergstrasse 4, 
      Garching, 85748, Germany.
FAU - Reichart, Florian
AU  - Reichart F
AD  - Institute for Advanced Study and Center of Integrated Protein Science (CIPSM), 
      Department of Chemistry, Technische Universitat Munchen, Lichtenbergstrasse 4, 
      Garching, 85748, Germany.
FAU - Kessler, Horst
AU  - Kessler H
AD  - Institute for Advanced Study and Center of Integrated Protein Science (CIPSM), 
      Department of Chemistry, Technische Universitat Munchen, Lichtenbergstrasse 4, 
      Garching, 85748, Germany.
FAU - Spatz, Joachim P
AU  - Spatz JP
AD  - Max Planck Institute for Medical Research, Department of Cellular Biophysics, 
      Jahnstrasse 29, Heidelberg, 69120, Germany.
FAU - Albiges-Rizo, Corinne
AU  - Albiges-Rizo C
AD  - Institut Albert Bonniot, Universite Joseph Fourier, INSERM U823, CNRS ERL 5284, 
      Grenoble Alpessite Sante, Grenoble Cedex, 09, F38042, France.
FAU - Mori, Giorgio
AU  - Mori G
AD  - University of Foggia, Department of Clinical and Experimental Medicine, viale 
      Pinto 1, Foggia, 71122, Italy.
FAU - Cavalcanti-Adam, Elisabetta Ada
AU  - Cavalcanti-Adam EA
AD  - Max Planck Institute for Medical Research, Department of Cellular Biophysics, 
      Jahnstrasse 29, Heidelberg, 69120, Germany. Electronic address: 
      eacavalcanti@mr.mpg.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201026
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Integrin alpha5beta1)
RN  - 0 (Integrin alphaVbeta3)
RN  - 0 (Ligands)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Cell Adhesion
MH  - *Gold
MH  - Integrin alpha5beta1
MH  - Integrin alphaVbeta3
MH  - Ligands
MH  - *Metal Nanoparticles
OTO - NOTNLM
OT  - BMP-2
OT  - Click chemistry
OT  - Focal adhesions
OT  - Integrins
OT  - Surface functionalization
EDAT- 2020/11/04 06:00
MHDA- 2021/05/25 06:00
CRDT- 2020/11/03 20:08
PHST- 2020/05/05 00:00 [received]
PHST- 2020/10/14 00:00 [revised]
PHST- 2020/10/21 00:00 [accepted]
PHST- 2020/11/04 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2020/11/03 20:08 [entrez]
AID - S0142-9612(20)30730-4 [pii]
AID - 10.1016/j.biomaterials.2020.120484 [doi]
PST - ppublish
SO  - Biomaterials. 2021 Jan;267:120484. doi: 10.1016/j.biomaterials.2020.120484. Epub 
      2020 Oct 26.