PMID- 33142158
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20201214
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 229
DP  - 2020 Dec
TI  - The molecular response mechanisms of a diatom Thalassiosira pseudonana to the 
      toxicity of BDE-47 based on whole transcriptome analysis.
PG  - 105669
LID - S0166-445X(20)30419-7 [pii]
LID - 10.1016/j.aquatox.2020.105669 [doi]
AB  - Polybrominated diphenyl ethers (PBDEs) are ubiquitously distributed persistent 
      organic pollutants (POPs) in marine environments. Phytoplankton are the entrance 
      of PBDEs entering to biotic environments from abiotic environments, while the 
      responding mechanisms of phytoplankton to PBDEs have not been full established. 
      Therefore, we chose the model diatom Thalassiosira pseudonana in this study, by 
      integrating whole transcriptome analysis with physiological-biochemical data, to 
      reveal the molecular responding mechanisms of T. pseudonana to the toxicity of 
      BDE-47. Our results indicated the changes of genes expressions correlated to the 
      physiological-biochemical changes, and there were multiple molecular mechanisms 
      of T. pseudonana responding to the toxicity of BDE-47: Gene expressions evidence 
      explained the suppression of light reaction and proved the occurrence of cellular 
      oxidative stress; In the meanwhile, up-regulations of genes in pathways involving 
      carbon metabolisms happened, including the Calvin cycle, glycolysis, TCA cycle, 
      fatty acid synthesis, and triacylglycerol synthesis; Lastly, DNA damage was found 
      and three outcome including DNA repair, cell cycle arrest and programmed cell 
      death (PCD) happened, which could finally inhibit the cell division and 
      population growth of T. pseudonana. This study presented the most complete 
      molecular responding mechanisms of phytoplankton cells to PBDEs, and provided 
      valuable information of various PBDEs-sensitive genes with multiple functions for 
      further research involving organic pollutants and phytoplankton.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhao, Yirong
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Tang, Xuexi
AU  - Tang X
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China. Electronic address: tangxx@ouc.edu.cn.
FAU - Lv, Mengchen
AU  - Lv M
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Liu, Qian
AU  - Liu Q
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Li, Jun
AU  - Li J
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Zhang, Bihan
AU  - Zhang B
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Li, Luying
AU  - Li L
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Zhang, Xinxin
AU  - Zhang X
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China. Electronic address: zhaoyan@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20201025
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Light-Harvesting Protein Complexes)
RN  - 0 (Photosystem II Protein Complex)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
RN  - 7440-44-0 (Carbon)
RN  - GAN16C9B8O (Glutathione)
RN  - N762921K75 (Nitrogen)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Carbon/metabolism
MH  - DNA Damage
MH  - Diatoms/drug effects/*genetics/physiology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Glutathione/metabolism
MH  - Halogenated Diphenyl Ethers/metabolism/*toxicity
MH  - Light-Harvesting Protein Complexes/genetics/metabolism
MH  - Metabolic Networks and Pathways/drug effects
MH  - Nitrogen/metabolism
MH  - Oxidative Stress/drug effects
MH  - Oxygen/metabolism
MH  - Photosynthesis/drug effects
MH  - Photosystem II Protein Complex/genetics/metabolism
MH  - Phytoplankton/drug effects/genetics
MH  - *Toxicity Tests
MH  - Transcriptome/genetics
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Carbon metabolisms
OT  - DNA damage
OT  - PBDEs
OT  - Photosynthesis
OT  - Thalassiosira pseudonana
OT  - Whole transcriptome analysis
EDAT- 2020/11/04 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/11/03 20:12
PHST- 2020/07/24 00:00 [received]
PHST- 2020/10/16 00:00 [revised]
PHST- 2020/10/21 00:00 [accepted]
PHST- 2020/11/04 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/11/03 20:12 [entrez]
AID - S0166-445X(20)30419-7 [pii]
AID - 10.1016/j.aquatox.2020.105669 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2020 Dec;229:105669. doi: 10.1016/j.aquatox.2020.105669. Epub 2020 
      Oct 25.