PMID- 33142449
OWN - NLM
STAT- MEDLINE
DCOM- 20210318
LR  - 20210318
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 99
IP  - 11
DP  - 2020 Nov
TI  - In vitro effects of 5 recombinant antigens of Eimeria maxima on maturation, 
      differentiation, and immunogenic functions of dendritic cells derived from 
      chicken spleen.
PG  - 5331-5343
LID - S0032-5791(20)30497-1 [pii]
LID - 10.1016/j.psj.2020.07.028 [doi]
AB  - Eimeria maxima possesses integral families of immunogenic constituents that 
      promote differentiation of immune cells during host-parasite interactions. 
      Dendritic cells (DCs) have an irreplaceable role in the modulation of the host 
      immunity. However, the selection of superlative antigen with immune stimulatory 
      efficacies on host DCs is lacking. In this study, 5 recombinant proteins of E. 
      maxima (Em), including Em14-3-3, rhomboid family domain containing proteins (ROM) 
      EmROM1 and EmROM2, microneme protein 2 (EmMIC2), and Em8 were identified to 
      stimulate chicken splenic derived DCs in vitro. The cultured populations were 
      incubated with recombinant proteins, and typical morphologies of stimulated DCs 
      were obtained. DC-associated markers major histocompatibility complex class II, 
      CD86, CD11c, and CD1.1, showed upregulatory expressions by flow cytometry assay. 
      Immunofluorescence assay revealed that recombinant proteins could bind with the 
      surface of chicken splenic derived DCs. Moreover, quantitative real-time PCR 
      results showed that distinct gene expressions of Toll-like receptors and Wnt 
      signaling pathway were upregulated after the coincubation of recombinant proteins 
      with DCs. The ELISA results indicated that the DCs produced a significant higher 
      level of interleukin (IL)-12 and interferon-gamma secretions after incubation with 
      recombinant proteins. While transforming growth factor-beta was significantly 
      increased with rEmROM1, rEmROM2, and rEmMIC2 as compared to control groups, and 
      IL-10 did not show significant alteration. Taken together, these results 
      concluded that among 5 potential recombinant antigens, rEm14-3-3 could promote 
      immunogenic functions of chicken splenic derived DCs more efficiently, which 
      might represent an effective molecule for inducing the host Th1-mediated immune 
      response against Eimeria infection.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - Haseeb, Muhammad
AU  - Haseeb M
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Lakho, Shakeel Ahmed
AU  - Lakho SA
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Huang, Jianmei
AU  - Huang J
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Hasan, Muhammad Waqqas
AU  - Hasan MW
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Ali-Ul-Husnain Naqvi, Muhammad
AU  - Ali-Ul-Husnain Naqvi M
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Zhou, Zhouyang
AU  - Zhou Z
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Yan, Ruofeng
AU  - Yan R
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Xu, Lixin
AU  - Xu L
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Song, Xiaokai
AU  - Song X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China.
FAU - Li, Xiangrui
AU  - Li X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 
      Jiangsu, PR China. Electronic address: lixiangrui@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200808
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antigens, Protozoan)
SB  - IM
MH  - Animals
MH  - *Antigens, Protozoan/genetics/immunology/pharmacology
MH  - *Cell Differentiation/drug effects
MH  - Chickens
MH  - *Dendritic Cells/drug effects
MH  - *Eimeria/chemistry/genetics
MH  - Female
MH  - *Immunity/drug effects
MH  - *Spleen/cytology
PMC - PMC7647736
OTO - NOTNLM
OT  - Eimeria maxima
OT  - chicken spleen
OT  - dendritic cell
OT  - immunogenic function
OT  - recombinant antigens
EDAT- 2020/11/05 06:00
MHDA- 2021/03/19 06:00
PMCR- 2020/08/08
CRDT- 2020/11/04 01:01
PHST- 2019/12/11 00:00 [received]
PHST- 2020/06/05 00:00 [revised]
PHST- 2020/07/22 00:00 [accepted]
PHST- 2020/11/04 01:01 [entrez]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/03/19 06:00 [medline]
PHST- 2020/08/08 00:00 [pmc-release]
AID - S0032-5791(20)30497-1 [pii]
AID - 10.1016/j.psj.2020.07.028 [doi]
PST - ppublish
SO  - Poult Sci. 2020 Nov;99(11):5331-5343. doi: 10.1016/j.psj.2020.07.028. Epub 2020 
      Aug 8.