PMID- 33143386 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201128 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 8 IP - 11 DP - 2020 Oct 30 TI - Activation of the Cell Wall Stress Response in Pseudomonas aeruginosa Infected by a Pf4 Phage Variant. LID - 10.3390/microorganisms8111700 [doi] LID - 1700 AB - Pseudomonas aeruginosa PAO1 has an integrated Pf4 prophage in its genome, encoding a relatively well-characterized filamentous phage, which contributes to the bacterial biofilm organization and maturation. Pf4 variants are considered as superinfectives when they can re-infect and kill the prophage-carrying host. Herein, the response of P. aeruginosa H103 to Pf4 variant infection was investigated. This phage variant caused partial lysis of the bacterial population and modulated H103 physiology. We show by confocal laser scanning microscopy that a Pf4 variant-infection altered P. aeruginosa H103 biofilm architecture either in static or dynamic conditions. Interestingly, in the latter condition, numerous cells displayed a filamentous morphology, suggesting a link between this phenotype and flow-related forces. In addition, Pf4 variant-infection resulted in cell envelope stress response, mostly mediated by the AlgU and SigX extracytoplasmic function sigma factors (ECFsigma). AlgU and SigX involvement may account, at least partly, for the enhanced expression level of genes involved in the biosynthesis pathways of two matrix exopolysaccharides (Pel and alginates) and bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) metabolism. FAU - Tortuel, Damien AU - Tortuel D AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Tahrioui, Ali AU - Tahrioui A AUID- ORCID: 0000-0003-4064-820X AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Rodrigues, Sophie AU - Rodrigues S AUID- ORCID: 0000-0001-8901-3032 AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Cambronel, Melyssa AU - Cambronel M AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Boukerb, Amine M AU - Boukerb AM AUID- ORCID: 0000-0002-8349-6739 AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Maillot, Olivier AU - Maillot O AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Verdon, Julien AU - Verdon J AD - Laboratoire Ecologie et Biologie des Interactions, Universite de Poitiers, UMR CNRS 7267 Poitiers, France. FAU - Bere, Emile AU - Bere E AD - Laboratoire Ecologie et Biologie des Interactions, Universite de Poitiers, UMR CNRS 7267 Poitiers, France. FAU - Nusser, Michael AU - Nusser M AD - Institute of Functional Interfaces, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany. FAU - Brenner-Weiss, Gerald AU - Brenner-Weiss G AD - Institute of Functional Interfaces, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany. FAU - David, Audrey AU - David A AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Azuama, Onyedikachi Cecil AU - Azuama OC AUID- ORCID: 0000-0001-5402-652X AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Feuilloley, Marc G J AU - Feuilloley MGJ AUID- ORCID: 0000-0001-6467-4336 AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Orange, Nicole AU - Orange N AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Lesouhaitier, Olivier AU - Lesouhaitier O AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Cornelis, Pierre AU - Cornelis P AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Chevalier, Sylvie AU - Chevalier S AUID- ORCID: 0000-0001-6489-438X AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. FAU - Bouffartigues, Emeline AU - Bouffartigues E AD - Laboratoire de Microbiologie Signaux et Microenvironnement, Universite de Rouen Normandie, Normandie Universite, LMSM EA4312 Evreux, France. LA - eng PT - Journal Article DEP - 20201030 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC7693463 OTO - NOTNLM OT - AlgU OT - Pf4 phage variant OT - Pseudomonas aeruginosa OT - SigX OT - biofilm OT - c-di-GMP OT - cell envelope OT - cell wall stress response OT - membrane fluidity COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2020/11/05 06:00 MHDA- 2020/11/05 06:01 PMCR- 2020/10/30 CRDT- 2020/11/04 01:03 PHST- 2020/10/06 00:00 [received] PHST- 2020/10/28 00:00 [revised] PHST- 2020/10/29 00:00 [accepted] PHST- 2020/11/04 01:03 [entrez] PHST- 2020/11/05 06:00 [pubmed] PHST- 2020/11/05 06:01 [medline] PHST- 2020/10/30 00:00 [pmc-release] AID - microorganisms8111700 [pii] AID - microorganisms-08-01700 [pii] AID - 10.3390/microorganisms8111700 [doi] PST - epublish SO - Microorganisms. 2020 Oct 30;8(11):1700. doi: 10.3390/microorganisms8111700.