PMID- 33143796
OWN - NLM
STAT- MEDLINE
DCOM- 20211214
LR  - 20211214
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 126
IP  - 4
DP  - 2021 Aug 28
TI  - Oleamide rescues tibialis anterior muscle atrophy of mice housed in small cages.
PG  - 481-491
LID - 10.1017/S0007114520004304 [doi]
AB  - Skeletal muscle atrophy causes decreased physical activity and increased risk of 
      metabolic diseases. We investigated the effects of oleamide 
      (cis-9,10-octadecanamide) treatment on skeletal muscle health. The plasma 
      concentration of endogenous oleamide was approximately 30 nm in male ddY mice 
      under normal physiological conditions. When the stable isotope-labelled oleamide 
      was orally administered to male ddY mice (50 mg/kg), the plasma concentration of 
      exogenous oleamide reached approximately 170 nm after 1 h. Male ddY mice were 
      housed in small cages (one-sixth of normal size) to enforce sedentary behaviour 
      and orally administered oleamide (50 mg/kg per d) for 4 weeks. Housing in small 
      cages decreased tibialis anterior (TA) muscle mass and the cross-sectional area 
      of the myofibres in TA muscle. Dietary oleamide alleviated the decreases in TA 
      muscle and resulted in plasma oleamide concentration of approximately 120 nm in 
      mice housed in small cages. Housing in small cages had no influence on the 
      phosphorylation levels of Akt serine/threonine kinase (Akt), mechanistic target 
      of rapamycin (mTOR) and ribosomal protein S6 kinase (p70S6K) in TA muscle; 
      nevertheless, oleamide increased the phosphorylation levels of the proteins. 
      Housing in small cages increased the expression of microtubule-associated protein 
      1 light chain 3 (LC3)-II and sequestosome 1 (p62), but not LC3-I, in TA muscle, 
      and oleamide reduced LC3-I, LC3-II and p62 expression levels. In C2C12 myotubes, 
      oleamide increased myotube diameter at >/=100 nm. Furthermore, the mTOR inhibitor, 
      Torin 1, suppressed oleamide-induced increases in myotube diameter and protein 
      synthesis. These results indicate that dietary oleamide rescued TA muscle atrophy 
      in mice housed in small cages, possibly by activating the phosphoinositide 
      3-kinase/Akt/mTOR signalling pathway and restoring autophagy flux.
FAU - Kobayashi, Yasuyuki
AU  - Kobayashi Y
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
FAU - Watanabe, Natsumi
AU  - Watanabe N
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
FAU - Kitakaze, Tomoya
AU  - Kitakaze T
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
FAU - Sugimoto, Keiichiro
AU  - Sugimoto K
AD  - Research and Development Center, Nagaoka Co. Ltd, Ibaraki, Osaka5670005, Japan.
AD  - Center for Research and Development of Bioresources, Osaka Prefecture University, 
      Sakai, Osaka5998531, Japan.
FAU - Izawa, Takeshi
AU  - Izawa T
AD  - Division of Veterinary Science, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Izumisano, Osaka5988531, Japan.
FAU - Kai, Kenji
AU  - Kai K
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
FAU - Harada, Naoki
AU  - Harada N
AUID- ORCID: 0000-0003-3846-388X
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
FAU - Yamaji, Ryoichi
AU  - Yamaji R
AUID- ORCID: 0000-0003-4729-3317
AD  - Division of Applied Life Sciences, Graduate School of Life and Environmental 
      Sciences, Osaka Prefecture University, Sakai, Osaka5998531, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201104
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Oleic Acids)
RN  - 7L25QK8BWO (oleylamide)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Housing, Animal
MH  - Male
MH  - Mice
MH  - Muscle Fibers, Skeletal/metabolism
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - *Muscular Atrophy/drug therapy
MH  - Oleic Acids/*pharmacology
MH  - *Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Mechanistic target of rapamycin signalling
OT  - Myotubes
OT  - Primary fatty acid amides
OT  - Sedentary behaviour
EDAT- 2020/11/05 06:00
MHDA- 2021/12/15 06:00
CRDT- 2020/11/04 05:32
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/11/04 05:32 [entrez]
AID - S0007114520004304 [pii]
AID - 10.1017/S0007114520004304 [doi]
PST - ppublish
SO  - Br J Nutr. 2021 Aug 28;126(4):481-491. doi: 10.1017/S0007114520004304. Epub 2020 
      Nov 4.