PMID- 33144432
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 34
IP  - 6
DP  - 2020 Nov-Dec
TI  - Comparison of TMA Technique and Routine Whole Slide Analysis in Evaluation of 
      Proliferative Markers Expression in Laryngeal Squamous Cell Cancer.
PG  - 3263-3270
LID - 10.21873/invivo.12163 [doi]
AB  - BACKGROUND/AIM: Comparison of the expression of Ki-67, MCM3, 5, 7 and MTI/II 
      proteins using immunohistochemistry (IHC) on whole section (WS) and tissue 
      microarray (TMA) of laryngeal squamous cell carcinoma (LSCC) samples. MATERIALS 
      AND METHODS: A total of 51 archival paraffin blocks of LSCC were used. TMAs were 
      prepared from 1.5 mm core punches. IHC reactions were performed using antibodies 
      against Ki-67, minichromosome maintenance proteins (MCM3, 5, 7) and 
      metallothionein (MTI/II). RESULTS: Spearman rank correlation test revealed 
      moderate positive correlation in the case of Ki-67: WS vs. TMA (r=0.38, p=0.07) 
      and strong positive correlation in regard to the rest of tested markers: MCM3, WS 
      vs. TMA (r=0.49, p=0.0004); MCM5, WS vs. TMA (r=0.61, p<0.0001); MCM7, WS vs. TMA 
      (r=0.59, p<0.0001); MTI/II, WS vs. TMA (r=0.66, p<0.0001). Mann Whitney U-test 
      showed no significant differences in the case of Ki-67 and MCM5. Moreover, 
      Bland-Altman test showed a low level of bias in regard to Ki-67, WS vs. TMA and 
      MCM5, WS vs. TMA. CONCLUSION: TMA may be an effective and reliable method of 
      assessment of Ki-67 and MCM5 expression in LSCC.
CI  - Copyright(c) 2020, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Ciesielska, Urszula
AU  - Ciesielska U
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland 
      urszula.ciesielska@umed.wroc.pl.
FAU - Piotrowska, Aleksandra
AU  - Piotrowska A
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Kobierzycki, Christopher
AU  - Kobierzycki C
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Pastuszewski, Wojciech
AU  - Pastuszewski W
AD  - Department of Otolaryngology, Municipal Hospital, Namsos, Norway.
FAU - Podhorska-Okolow, Marzenna
AU  - Podhorska-Okolow M
AD  - Division of Ultrastructure Research, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
AD  - Department of Physiotherapy, Wroclaw University School of Physical Education, 
      Wroclaw, Poland.
FAU - Nowinska, Katarzyna
AU  - Nowinska K
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Biomarkers, Tumor
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/genetics
MH  - *Metallothionein
MH  - Squamous Cell Carcinoma of Head and Neck
PMC - PMC7811677
OTO - NOTNLM
OT  - 5
OT  - 7
OT  - Ki-67
OT  - MCM3
OT  - MTI/II
OT  - TMA
OT  - Tissue microarray
OT  - laryngeal cancer
COIS- The Authors declare that there are no conflicts of interest regarding this study.
EDAT- 2020/11/05 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/11/03
CRDT- 2020/11/04 05:36
PHST- 2020/07/17 00:00 [received]
PHST- 2020/08/03 00:00 [revised]
PHST- 2020/08/05 00:00 [accepted]
PHST- 2020/11/04 05:36 [entrez]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/11/03 00:00 [pmc-release]
AID - 34/6/3263 [pii]
AID - 10.21873/invivo.12163 [doi]
PST - ppublish
SO  - In Vivo. 2020 Nov-Dec;34(6):3263-3270. doi: 10.21873/invivo.12163.